ID A8JKF6_HELVI Unreviewed; 696 AA.
AC A8JKF6;
DT 04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT 04-DEC-2007, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=tyrosinase {ECO:0000256|ARBA:ARBA00011906};
DE EC=1.14.18.1 {ECO:0000256|ARBA:ARBA00011906};
GN ORFNames=B5V51_7771 {ECO:0000313|EMBL:PCG80455.1};
OS Heliothis virescens (Tobacco budworm moth).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Noctuoidea;
OC Noctuidae; Heliothinae; Heliothis.
OX NCBI_TaxID=7102 {ECO:0000313|EMBL:ABM65701.1};
RN [1] {ECO:0000313|EMBL:ABM65701.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=18839417; DOI=10.1002/arch.20274;
RA Shelby K.S., Popham H.J.;
RT "Cloning and characterization of the secreted hemocytic prophenoloxidases
RT of Heliothis virescens.";
RL Arch. Insect Biochem. Physiol. 69:127-142(2008).
RN [2] {ECO:0000313|EMBL:PCG80455.1, ECO:0000313|Proteomes:UP000218220}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HvINT- {ECO:0000313|EMBL:PCG80455.1};
RC TISSUE=Whole body {ECO:0000313|EMBL:PCG80455.1};
RA Fritz M.L., Deyonke A.M., Papanicolaou A., Micinski S., Westbrook J.,
RA Gould F.;
RT "Contemporary evolution of a Lepidopteran species, Heliothis virescens, in
RT response to modern agricultural practices.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504,
CC ChEBI:CHEBI:57924; EC=1.14.18.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000426};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924,
CC ChEBI:CHEBI:58315; EC=1.14.18.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001038};
CC -!- COFACTOR:
CC Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC Evidence={ECO:0000256|ARBA:ARBA00001973};
CC -!- SIMILARITY: Belongs to the tyrosinase family.
CC {ECO:0000256|ARBA:ARBA00009928}.
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DR EMBL; EF044308; ABM65701.1; -; mRNA.
DR EMBL; NWSH01000035; PCG80455.1; -; Genomic_DNA.
DR AlphaFoldDB; A8JKF6; -.
DR STRING; 7102.A8JKF6; -.
DR Proteomes; UP000218220; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004503; F:tyrosinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006582; P:melanin metabolic process; IEA:UniProt.
DR GO; GO:1901564; P:organonitrogen compound metabolic process; IEA:UniProt.
DR Gene3D; 1.10.1280.10; Di-copper center containing domain from catechol oxidase; 1.
DR Gene3D; 2.60.40.1520; Hemocyanin, C-terminal domain; 1.
DR Gene3D; 1.20.1370.10; Hemocyanin, N-terminal domain; 1.
DR InterPro; IPR008922; Di-copper_centre_dom_sf.
DR InterPro; IPR013788; Hemocyanin/hexamerin.
DR InterPro; IPR000896; Hemocyanin/hexamerin_mid_dom.
DR InterPro; IPR005203; Hemocyanin_C.
DR InterPro; IPR037020; Hemocyanin_C_sf.
DR InterPro; IPR005204; Hemocyanin_N.
DR InterPro; IPR036697; Hemocyanin_N_sf.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR002227; Tyrosinase_Cu-bd.
DR PANTHER; PTHR11511; LARVAL STORAGE PROTEIN/PHENOLOXIDASE; 1.
DR PANTHER; PTHR11511:SF4; PHENOLOXIDASE 2-RELATED; 1.
DR Pfam; PF03723; Hemocyanin_C; 1.
DR Pfam; PF00372; Hemocyanin_M; 1.
DR Pfam; PF03722; Hemocyanin_N; 1.
DR PRINTS; PR00187; HAEMOCYANIN.
DR SUPFAM; SSF48056; Di-copper centre-containing domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF48050; Hemocyanin, N-terminal domain; 1.
DR PROSITE; PS00209; HEMOCYANIN_1; 1.
DR PROSITE; PS00210; HEMOCYANIN_2; 1.
DR PROSITE; PS00498; TYROSINASE_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:ABM65701.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000218220};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 402..413
FT /note="Tyrosinase copper-binding"
FT /evidence="ECO:0000259|PROSITE:PS00498"
SQ SEQUENCE 696 AA; 79889 MW; 43AFB93AAA055818 CRC64;
MADEDRIVES FKLLFDRPNE PLITPKGDKK ALFQLTEKLV PPDYVNNGVE LNDRFGDDAT
ERIPLKTLDK YPEFRLASQL PADADFSLFL PKHQDMATEV IDALLGVPEN QLQDFLSTCV
FARGNLNPQL FNYCYSVALM HRRDTRNVPI QNFAETFPSK FMDSQVFQQA RESAAVIPQS
VPRTPIIIPR DYTATDLEEE HRLAYWREDI GVNLHHWHWH LVYPFTASQR SIVAKDRRGE
LFFYMHQQLI ARYNGERLNN SLKRVKKFSN WREPIPEAYF PKLDSLTSSR GWPPRQANMQ
WQDLNRPVDG LNVTISDMER WRRNVEEAIS TGTVTNADGT TSPLDIDILG NMLEASILSP
NRELYGSIHN NGHSFSAYIH DPTHRYLESF GVIADEATTM RDPFFFRWHA WIDDICQKHK
ESSYVRPYTR SELENPGVQV TSVSVETEGG APANTLNTFW MSSDVDLSRG LDFSDRGPVY
ARFTHLNNRP FRYVINVNNT GNARRTTVRI FIAPKFDERN LAWALSDHRK MFIEMDRFVQ
PLNAGQNTIV RLSTQSSLTI PFEQTFRDLS AQSNDPRRPN LAEFNFCGCG WPQHMLVPKG
TEAGAAYQLF VMLSNYDLDS VDQPDGSQLS CVEASSFCGL KDRKYPDRRA MGFPFDRPSS
TATNIEDFIL PNMGLQDITI RLSNTTEPNP RNPRTN
//
