ID   A9GYV5_SHEPW            Unreviewed;       526 AA.
AC   A9GYV5;
DT   05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   05-FEB-2008, sequence version 1.
DT   24-JAN-2024, entry version 59.
DE   RecName: Full=Isocitrate lyase {ECO:0000256|ARBA:ARBA00017446};
DE   AltName: Full=Isocitrase {ECO:0000256|ARBA:ARBA00031022};
DE   AltName: Full=Isocitratase {ECO:0000256|ARBA:ARBA00031921};
GN   Name=icl {ECO:0000313|EMBL:CAO78894.1};
OS   Shewanella piezotolerans (strain WP3 / JCM 13877).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=225849 {ECO:0000313|EMBL:CAO78894.1};
RN   [1] {ECO:0000313|EMBL:CAO78894.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Type strain: WP3 {ECO:0000313|EMBL:CAO78894.1};
RA   Li J., Xiao X., Wang F.;
RT   "Expression and characterization of isocitrate lyase of WP3, a
RT   psychrotolerant and piezotolerant Shewanella bacterium.";
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate = glyoxylate + succinate;
CC         Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031,
CC         ChEBI:CHEBI:36655; EC=4.1.3.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00023531};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC       Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AM773235; CAO78894.1; -; Genomic_DNA.
DR   AlphaFoldDB; A9GYV5; -.
DR   GO; GO:0004451; F:isocitrate lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   CDD; cd00377; ICL_PEPM; 1.
DR   Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR   InterPro; IPR039556; ICL/PEPM.
DR   InterPro; IPR006254; Isocitrate_lyase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR   PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR   PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR   Pfam; PF00463; ICL; 3.
DR   PIRSF; PIRSF001362; Isocit_lyase; 3.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   4: Predicted;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:CAO78894.1};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3}.
FT   ACT_SITE        218
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001362-1"
FT   BINDING         180
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
SQ   SEQUENCE   526 AA;  58419 MW;  930053CECAC6F295 CRC64;
     MTNYRTNIDE AATLINAEGS AWNAINPESV ARMRLQNRFK TGLDIAKYTA KIMREDMDNY
     DKDSSQYTQS LGCWHGFIGQ QKMISIKKHL LTTKRRYLYL SGWMVAALRS EFGPLPDQSM
     HEKTSVASLI KELYTFLRQA DARELGGLFR QLDGASDADK PAIQAKIDNF ETHVVPIIAD
     IDAGFGNEEA TYLMAKQMIE AGACCIQLEN QVSDVKQCGH QDGKVTVPHV EFLAKINAVR
     YAFLELGIDD GVIVARTDSL GAGLTQKIAV TNEAGDLGEQ YNSFLEVEAI DAANLANGDV
     VFQQNGQLVK PSRLPNGLFK FRAGTGEERC VLDCITSLQN GADLLWIETE KPHVSQIGGM
     VNEIRKTIPN AKLVYNNSPS FNWTLNFRQQ VFDAMKEAGQ DVSAYNRDQL MSVDYDSTEL
     AIQADEKIRT FQADAAREAG IFHHLITLPT YHTAALSTDN LAKEYFGDQG MLGYVANVQR
     KEIRQGIACV KHQNMAGSDM GDDHKEYFSG DQALKASGKD NTMNQF
//