ID ACSA_CRYPV Reviewed; 694 AA.
AC Q27549;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 13-SEP-2023, entry version 77.
DE RecName: Full=Acetyl-coenzyme A synthetase;
DE EC=6.2.1.1;
DE AltName: Full=Acetate--CoA ligase;
DE AltName: Full=Acyl-activating enzyme;
GN Name=ACS;
OS Cryptosporidium parvum.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Cryptosporidiidae; Cryptosporidium.
OX NCBI_TaxID=5807;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KSU-1;
RX PubMed=8636846; DOI=10.2307/3284079;
RA Khramtsov N.V., Blunt D.S., Montelone B.A., Upton S.J.;
RT "The putative acetyl-CoA synthetase gene of Cryptosporidium parvum and a
RT new conserved protein motif in acetyl-CoA synthetases.";
RL J. Parasitol. 82:423-427(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate;
CC Xref=Rhea:RHEA:23176, ChEBI:CHEBI:30089, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:456215; EC=6.2.1.1;
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; U24082; AAC47128.1; -; Genomic_DNA.
DR AlphaFoldDB; Q27549; -.
DR SMR; Q27549; -.
DR VEuPathDB; CryptoDB:cgd1_3710; -.
DR VEuPathDB; CryptoDB:CPATCC_0035560; -.
DR GO; GO:0003987; F:acetate-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0016208; F:AMP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0019427; P:acetyl-CoA biosynthetic process from acetate; IEA:InterPro.
DR CDD; cd05966; ACS; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR011904; Ac_CoA_lig.
DR InterPro; IPR032387; ACAS_N.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR NCBIfam; TIGR02188; Ac_CoA_lig_AcsA; 1.
DR PANTHER; PTHR24095; ACETYL-COENZYME A SYNTHETASE; 1.
DR PANTHER; PTHR24095:SF14; ACETYL-COENZYME A SYNTHETASE 1; 1.
DR Pfam; PF16177; ACAS_N; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding.
FT CHAIN 1..694
FT /note="Acetyl-coenzyme A synthetase"
FT /id="PRO_0000208410"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 229..232
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 347
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 423..425
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 447..452
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 536
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 551
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 559
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 562
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 628
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
SQ SEQUENCE 694 AA; 77971 MW; 5BA42E16E9AEE63C CRC64;
MSDKRPRSPC SNNNDELNDS SVLTENMDTV ATRSKNLPFH ETYKSKPTPA DKPYRVDGID
KYKELYEQSI RDPEGFWSQM ARKELRWLRD FTKVRSSGTC LQDRLAWFLN GKLNVCDNCV
DRWAEIQPNT TALIWEGDDP SSIRHISYIE LFRNVCKMAN VLKRFGIKKG DSVGIYMPMI
PETIYTMLAC ARIGAVHMVV FAGFAAQNLL ERLVNARCKI VVTADQGSRG KKIINLKDVV
DKALEKIPEI KTCIVFRHLN GPIEFVKGRD FDGETLMRSE KPYCPLEDMD SEDPLFYLYT
SGSTGTPKGV QHSTAGYLLY AAVTQKYLFN IHPGDIFGCA GDIGWITGHS YLVYAPLCNG
ITTLIFEGVP TYPNAGRYWE MVERHRITHF YAAPTAIRTL KRLGDDFVKK HDRSSLRVLG
SVGEPINPSA WRWYHSVVGE ERCSIVDTYW QTETGGIVIA PIPGCFDTKP GSATFPFFGI
EPAILDPDTG KEIDGPGSGV LCIKNSWPGM FRGIFGAHYL HEIYTKPFPK YYFTGDGVLR
DQDGYLWITG RIDDTINVSG HRLSSKEIED ALTNHFGIAE AAAVAIDHDV KGNALVCFVV
LKDSGNRTFD LNNSSPHPFE YELRMCVRTQ IGPVATPDHI IVVENIPKTR SGKVVRRLLR
KIATGCNDYG DISTVANPEC IKSIESSWAQ YLKR
//
