ID AEE21_ARATH Reviewed; 546 AA.
AC Q9SFW5; Q9SGQ5;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 27-MAR-2024, entry version 123.
DE RecName: Full=Probable acyl-activating enzyme 21;
DE EC=6.2.1.-;
GN Name=AEE21; OrderedLocusNames=At1g76290; ORFNames=F15M4.21, T23E18.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12805634; DOI=10.1104/pp.103.020552;
RA Shockey J.M., Fulda M.S., Browse J.;
RT "Arabidopsis contains a large superfamily of acyl-activating enzymes.
RT Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme
RT a synthetases.";
RL Plant Physiol. 132:1065-1076(2003).
CC -!- FUNCTION: May act as an acid--thiol ligase that activates carboxylic
CC acids by forming acyl-CoAs. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF17636.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009978; AAF17636.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC012394; AAF16671.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35821.1; -; Genomic_DNA.
DR PIR; D96790; D96790.
DR RefSeq; NP_177756.1; NM_106279.2.
DR AlphaFoldDB; Q9SFW5; -.
DR SMR; Q9SFW5; -.
DR STRING; 3702.Q9SFW5; -.
DR iPTMnet; Q9SFW5; -.
DR PaxDb; 3702-AT1G76290-1; -.
DR ProteomicsDB; 244744; -.
DR EnsemblPlants; AT1G76290.1; AT1G76290.1; AT1G76290.
DR GeneID; 843962; -.
DR Gramene; AT1G76290.1; AT1G76290.1; AT1G76290.
DR KEGG; ath:AT1G76290; -.
DR Araport; AT1G76290; -.
DR TAIR; AT1G76290; -.
DR eggNOG; KOG1176; Eukaryota.
DR HOGENOM; CLU_000022_59_5_1; -.
DR InParanoid; Q9SFW5; -.
DR OMA; VCLREVN; -.
DR OrthoDB; 674625at2759; -.
DR PhylomeDB; Q9SFW5; -.
DR BioCyc; ARA:AT1G76290-MONOMER; -.
DR PRO; PR:Q9SFW5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SFW5; baseline and differential.
DR Genevisible; Q9SFW5; AT.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR CDD; cd05915; ttLC_FACS_like; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR PANTHER; PTHR43859; ACYL-ACTIVATING ENZYME; 1.
DR PANTHER; PTHR43859:SF5; ISOVALERATE--COA LIGASE AAE2-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism; Ligase; Lipid metabolism; Reference proteome.
FT CHAIN 1..546
FT /note="Probable acyl-activating enzyme 21"
FT /id="PRO_0000415731"
SQ SEQUENCE 546 AA; 60494 MW; 3CBA5944955342AB CRC64;
MEGTMRCSAN YVPLSPISFL ERAAVVFGSR TSVVYGDIQY TWHQTRDRCV RLASALSDLG
LSRHDVVAAL APNVPALCEL YFGAPMAGAV LCVLNTTFDS QMLAMALEKT KPKVFFVDSE
FLSVAEESLS LLSNIEEKPL IITITENPTE QSKYEQYEDF LSTGNPNFKP IRPVDECDPI
ALNFTSGTTS TPKCVVYSHR GAYLNATAVG VMNEMKPMPV YLCTVPMYHC SGWCYIWTVT
AFGGVIVCLR EVNDEVIFDS IVKHKVTNFG GSPPVLNMIA NARDSVKKSF PWTVQVMSGG
SSPPEVMLKL KKLGFKVMMA YGCSEVYGLG TACLWMPEWE TLPEEESLRL KARDGLNHFA
KEAVDVLDPT TMKSVPHDGK TIRVIALRGN TVMSGYFKDK EATEAAFRGG WYWSRDMGVI
DPDGYIQFKD RSQDVITCGG EIVGSKEIEG ILYSHPAVYD AGVVGRPDET LGESMCAFVK
LKEGAEAREE EIIEFCKRKL GNKNMKMIPK TVVFSDVPKT PTGKIRKNVL RKMAKDMGYV
QLRAVE
//
