ID ANP7_ZOAAM Reviewed; 64 AA.
AC P19611;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 22-FEB-2023, entry version 82.
DE RecName: Full=Ice-structuring protein SP4(HPLC 7);
DE Short=ISP SP4(HPLC 7);
DE AltName: Full=Antifreeze protein SP4(HPLC 7);
OS Zoarces americanus (Ocean pout) (Macrozoarces americanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Zoarcinae;
OC Zoarces.
OX NCBI_TaxID=8199;
RN [1]
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=3403560; DOI=10.1016/s0021-9258(18)37891-8;
RA Hew C.-L., Wang N.-C., Joshi S., Fletcher G.L., Scott G.K., Hayes P.H.,
RA Buettner B., Davies P.L.;
RT "Multiple genes provide the basis for antifreeze protein diversity and
RT dosage in the ocean pout, Macrozoarces americanus.";
RL J. Biol. Chem. 263:12049-12055(1988).
CC -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC sea water temperatures. Lowers the blood freezing point. Binds to
CC nascent ice crystals and prevents further growth (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3403560}.
CC -!- TISSUE SPECIFICITY: Detected in blood serum (at protein level).
CC {ECO:0000269|PubMed:3403560}.
CC -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR PIR; G31075; G31075.
DR AlphaFoldDB; P19611; -.
DR SMR; P19611; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR CDD; cd11617; Antifreeze_III; 1.
DR Gene3D; 3.90.1210.10; Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain; 1.
DR InterPro; IPR006190; AFP_Neu5c_C.
DR InterPro; IPR036732; AFP_Neu5c_C_sf.
DR InterPro; IPR006013; Antifreeze_III.
DR InterPro; IPR013974; SAF.
DR Pfam; PF08666; SAF; 1.
DR PRINTS; PR00357; ANTIFREEZIII.
DR SMART; SM00858; SAF; 1.
DR SUPFAM; SSF51269; AFP III-like domain; 1.
DR PROSITE; PS50844; AFP_LIKE; 1.
PE 1: Evidence at protein level;
KW Antifreeze protein; Direct protein sequencing; Secreted.
FT CHAIN 1..64
FT /note="Ice-structuring protein SP4(HPLC 7)"
FT /id="PRO_0000155158"
FT DOMAIN 3..62
FT /note="AFP-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT SITE 8
FT /note="Important for ice-binding"
FT /evidence="ECO:0000250"
FT SITE 13
FT /note="Important for ice-binding"
FT /evidence="ECO:0000250"
FT SITE 17
FT /note="Important for ice-binding"
FT /evidence="ECO:0000250"
FT SITE 43
FT /note="Important for ice-binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 64 AA; 6846 MW; FD2E27DB328C423A CRC64;
SQSVVATRLI PMNTALTPAM MEGKVTNPIG IPFAEMSQIV GKQVNRIVAK GQTLMPNMVK
TYAA
//
