ID APF2_GIBF5 Reviewed; 389 AA.
AC S0DPL8;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Apicidin F cluster transcription factor apf2 {ECO:0000303|PubMed:25058475};
DE AltName: Full=Apicidin F synthesis protein 2 {ECO:0000303|PubMed:25058475};
GN Name=apf2 {ECO:0000303|PubMed:25058475}; ORFNames=FFUJ_00012;
OS Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS and foot rot disease fungus) (Fusarium fujikuroi).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=1279085;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND FUNCTION.
RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA Tudzynski B.;
RT "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT novel metabolites.";
RL PLoS Pathog. 9:E1003475-E1003475(2013).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=24195442; DOI=10.1021/np4006053;
RA von Bargen K.W., Niehaus E.M., Bergander K., Brun R., Tudzynski B.,
RA Humpf H.U.;
RT "Structure elucidation and antimalarial activity of apicidin F: an
RT apicidin-like compound produced by Fusarium fujikuroi.";
RL J. Nat. Prod. 76:2136-2140(2013).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, DOMAIN, AND SUBCELLULAR LOCATION.
RX PubMed=25058475; DOI=10.1371/journal.pone.0103336;
RA Niehaus E.M., Janevska S., von Bargen K.W., Sieber C.M., Harrer H.,
RA Humpf H.U., Tudzynski B.;
RT "Apicidin F: characterization and genetic manipulation of a new secondary
RT metabolite gene cluster in the rice pathogen Fusarium fujikuroi.";
RL PLoS ONE 9:E103336-E103336(2014).
CC -!- FUNCTION: Transcription factor that regulates the expression of the
CC gene cluster that mediates the biosynthesis of apicidin F
CC (PubMed:23825955, PubMed:25058475). Binds to the eight-base-pair motif
CC 5'-TGACGTGA-3' called the 'Api-box' that is found in all promoters of
CC the apicidin F cluster except in the promoter region of apf2 itself
CC (PubMed:25058475). {ECO:0000269|PubMed:23825955,
CC ECO:0000269|PubMed:25058475}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25058475}.
CC -!- DOMAIN: Contains a basic DNA-binding region at the N-terminus which is
CC usually found in bZIP transcription factors, but does not contain the
CC characteristic leucine zipper domain (PubMed:25058475). Instead, four
CC C-terminal ankyrin repeats were identified that were shown to confer
CC protein-protein interaction of regulatory proteins (PubMed:25058475).
CC {ECO:0000305|PubMed:25058475}.
CC -!- DISRUPTION PHENOTYPE: Leads to the loss of apicidin F production
CC (PubMed:25058475). {ECO:0000269|PubMed:25058475}.
CC -!- BIOTECHNOLOGY: Apicidin F, like the other known apicidins, is a cyclic
CC tetrapeptides with anti-malarial properties via histone deacetylase
CC inhibitory activity (PubMed:24195442). {ECO:0000269|PubMed:24195442}.
CC -!- SIMILARITY: Belongs to the bZIP family. Highly divergent.
CC {ECO:0000305}.
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DR EMBL; HF679023; CCT63352.1; -; Genomic_DNA.
DR AlphaFoldDB; S0DPL8; -.
DR SMR; S0DPL8; -.
DR STRING; 1279085.S0DPL8; -.
DR EnsemblFungi; CCT63352; CCT63352; FFUJ_00012.
DR VEuPathDB; FungiDB:FFUJ_00012; -.
DR HOGENOM; CLU_709899_0_0_1; -.
DR OrthoDB; 1880983at2759; -.
DR Proteomes; UP000016800; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR CDD; cd14688; bZIP_YAP; 1.
DR Gene3D; 1.20.5.170; -; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR046347; bZIP_sf.
DR PANTHER; PTHR24178:SF9; ANK_REP_REGION DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR24178; MOLTING PROTEIN MLT-4; 1.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 1.
DR SMART; SM00248; ANK; 4.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF57959; Leucine zipper domain; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 4.
PE 1: Evidence at protein level;
KW ANK repeat; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..389
FT /note="Apicidin F cluster transcription factor apf2"
FT /id="PRO_0000437168"
FT REPEAT 241..270
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 274..303
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 307..336
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REPEAT 357..386
FT /note="ANK 4"
FT /evidence="ECO:0000255"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 12..38
FT /note="Basic DNA-binding region"
FT /evidence="ECO:0000305|PubMed:25058475"
FT REGION 65..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 219..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 389 AA; 42375 MW; C1A6BA2C2EE4CD1B CRC64;
MSPPSQDWST ITDANERRKA QNRVAQRNYR SRQKLRVELA EAILYDMPSW RTAVGLKRKR
WLETPQSVED HDTNPDSATP KPSLSSQVIQ YHEEGQLVQN PTDFSMSADA DVTADVSSGG
ESSDLSLGVF TPGDDWMNAE IDWTAAMTLA GDTSASEPDL RHRAVGPGND IHAQPPVSIP
VQAASGQHRT LTAPTPAESM SLRTEPPETA VITVTSNREV PNLGDSNEGS RSISTTKQPS
EFKTPVLTAI AKGKLDIARL LISSGANIDT RDMHGRTNLH YAVSRSDAKT VRSLLELGAD
LLMSDASGVT ALHMAVGADD QDMVKLLLGW CEQQDRTASS TDHKTTLKQC INSRDGQNMT
PLHLCVVMER MDMLKILLDY GADVNIGCD
//
