ID APT_FRATT Reviewed; 175 AA.
AC Q5NII9;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE RecName: Full=Adenine phosphoribosyltransferase {ECO:0000255|HAMAP-Rule:MF_00004};
DE Short=APRT {ECO:0000255|HAMAP-Rule:MF_00004};
DE EC=2.4.2.7 {ECO:0000255|HAMAP-Rule:MF_00004};
GN Name=apt {ECO:0000255|HAMAP-Rule:MF_00004}; OrderedLocusNames=FTT_0078;
OS Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=177416;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCHU S4 / Schu 4;
RX PubMed=15640799; DOI=10.1038/ng1499;
RA Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H.,
RA Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D.,
RA Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S.,
RA Sjoestedt A., Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W.,
RA Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W.;
RT "The complete genome sequence of Francisella tularensis, the causative
RT agent of tularemia.";
RL Nat. Genet. 37:153-159(2005).
CC -!- FUNCTION: Catalyzes a salvage reaction resulting in the formation of
CC AMP, that is energically less costly than de novo synthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00004}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate +
CC adenine; Xref=Rhea:RHEA:16609, ChEBI:CHEBI:16708, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58017, ChEBI:CHEBI:456215; EC=2.4.2.7;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00004};
CC -!- PATHWAY: Purine metabolism; AMP biosynthesis via salvage pathway; AMP
CC from adenine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00004}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00004}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00004}.
CC -!- SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase
CC family. {ECO:0000255|HAMAP-Rule:MF_00004}.
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DR EMBL; AJ749949; CAG44711.1; -; Genomic_DNA.
DR RefSeq; WP_003028608.1; NZ_CP010290.1.
DR RefSeq; YP_169153.1; NC_006570.2.
DR PDB; 5YW2; X-ray; 2.28 A; A/B/C/D=1-175.
DR PDB; 5YW5; X-ray; 1.90 A; A/B/C/D=1-175.
DR PDBsum; 5YW2; -.
DR PDBsum; 5YW5; -.
DR AlphaFoldDB; Q5NII9; -.
DR SMR; Q5NII9; -.
DR STRING; 177416.FTT_0078; -.
DR DNASU; 3191768; -.
DR EnsemblBacteria; CAG44711; CAG44711; FTT_0078.
DR KEGG; ftu:FTT_0078; -.
DR eggNOG; COG0503; Bacteria.
DR OrthoDB; 9803963at2; -.
DR BRENDA; 2.4.2.7; 14771.
DR UniPathway; UPA00588; UER00646.
DR Proteomes; UP000001174; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003999; F:adenine phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006168; P:adenine salvage; IEA:UniProtKB-UniRule.
DR GO; GO:0044209; P:AMP salvage; IEA:UniProtKB-UniPathway.
DR GO; GO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW.
DR CDD; cd06223; PRTases_typeI; 1.
DR Gene3D; 3.40.50.2020; -; 1.
DR HAMAP; MF_00004; Aden_phosphoribosyltr; 1.
DR InterPro; IPR005764; Ade_phspho_trans.
DR InterPro; IPR000836; PRibTrfase_dom.
DR InterPro; IPR029057; PRTase-like.
DR NCBIfam; TIGR01090; apt; 1.
DR PANTHER; PTHR32315; ADENINE PHOSPHORIBOSYLTRANSFERASE; 1.
DR PANTHER; PTHR32315:SF3; ADENINE PHOSPHORIBOSYLTRANSFERASE; 1.
DR Pfam; PF00156; Pribosyltran; 1.
DR SUPFAM; SSF53271; PRTase-like; 1.
DR PROSITE; PS00103; PUR_PYR_PR_TRANSFER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Glycosyltransferase; Purine salvage;
KW Reference proteome; Transferase.
FT CHAIN 1..175
FT /note="Adenine phosphoribosyltransferase"
FT /id="PRO_0000149384"
FT HELIX 1..7
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 23..25
FT /evidence="ECO:0007829|PDB:5YW5"
FT HELIX 27..31
FT /evidence="ECO:0007829|PDB:5YW5"
FT HELIX 35..49
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 55..60
FT /evidence="ECO:0007829|PDB:5YW5"
FT HELIX 63..75
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 92..98
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 100..109
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 118..128
FT /evidence="ECO:0007829|PDB:5YW5"
FT HELIX 130..141
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 145..155
FT /evidence="ECO:0007829|PDB:5YW5"
FT HELIX 160..163
FT /evidence="ECO:0007829|PDB:5YW5"
FT TURN 164..166
FT /evidence="ECO:0007829|PDB:5YW5"
FT STRAND 169..175
FT /evidence="ECO:0007829|PDB:5YW5"
SQ SEQUENCE 175 AA; 18795 MW; 253CEA24588DA900 CRC64;
MNLDFIKSKI AAVPDFPKPG IMFRDITPLL ADPQGLRKTA EAMAQELKNK GIQPTIVAGT
ESRGFIFGVA LAEVLGLGFV PVRKPGKLPR ATYSVKYDLE YGSDSLEIHQ DAFKVTDEVL
VVDDLLATGG TAKATVDLIE KTQAKVAGLI FVMELDGLGG REVLAGYNVS ALIKF
//
