ID ARHGA_MOUSE Reviewed; 1345 AA.
AC Q8C033; Q5DU38; Q80VH8; Q8BW76; Q922S7;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 24-JAN-2024, entry version 151.
DE RecName: Full=Rho guanine nucleotide exchange factor 10;
GN Name=Arhgef10; Synonyms=Kiaa0294;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb, and Oviduct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Fetal brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND ALTERNATIVE
RP SPLICING.
RX PubMed=14508709; DOI=10.1086/378159;
RA Verhoeven K., De Jonghe P., Van de Putte T., Nelis E., Zwijsen A.,
RA Verpoorten N., De Vriendt E., Jacobs A., Van Gerwen V., Francis A.,
RA Ceuterick C., Huylebroeck D., Timmerman V.;
RT "Slowed conduction and thin myelination of peripheral nerves associated
RT with mutant rho Guanine-nucleotide exchange factor 10.";
RL Am. J. Hum. Genet. 73:926-932(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157 AND SER-1262, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in developmental myelination of peripheral
CC nerves. {ECO:0000269|PubMed:14508709}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=Q8C033-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C033-2; Sequence=VSP_010707;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:14508709}.
CC -!- DEVELOPMENTAL STAGE: Found in the neuroepithelium of the meninges at
CC 8.5 dpc, and in the roof plate of the rhombencephalon at 9.5 dpc. In
CC 12.5 dpc embryos, it is ubiquitously expressed, with a pronounced
CC expression in the neuroepithelium of brain vesicles, the neural tube,
CC the ganglia and the neural layer of the retina.
CC {ECO:0000269|PubMed:14508709}.
CC -!- PTM: Methylated at Gln-1314 by N6AMT1. {ECO:0000250|UniProtKB:O15013}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH59212.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH66074.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC27875.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD90400.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK032452; BAC27875.1; ALT_INIT; mRNA.
DR EMBL; AK054038; BAC35629.2; -; mRNA.
DR EMBL; AK220332; BAD90400.1; ALT_INIT; mRNA.
DR EMBL; BC006842; AAH06842.1; -; mRNA.
DR EMBL; BC047430; AAH47430.1; -; mRNA.
DR EMBL; BC059212; AAH59212.1; ALT_INIT; mRNA.
DR EMBL; BC066074; AAH66074.1; ALT_INIT; mRNA.
DR CCDS; CCDS40240.1; -. [Q8C033-1]
DR CCDS; CCDS40241.1; -. [Q8C033-2]
DR RefSeq; NP_001032825.1; NM_001037736.2. [Q8C033-2]
DR RefSeq; NP_766339.2; NM_172751.3. [Q8C033-1]
DR AlphaFoldDB; Q8C033; -.
DR SMR; Q8C033; -.
DR BioGRID; 231493; 10.
DR STRING; 10090.ENSMUSP00000081225; -.
DR GlyGen; Q8C033; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8C033; -.
DR PhosphoSitePlus; Q8C033; -.
DR jPOST; Q8C033; -.
DR MaxQB; Q8C033; -.
DR PaxDb; 10090-ENSMUSP00000081225; -.
DR ProteomicsDB; 277283; -. [Q8C033-1]
DR ProteomicsDB; 277284; -. [Q8C033-2]
DR Pumba; Q8C033; -.
DR Antibodypedia; 8026; 187 antibodies from 31 providers.
DR DNASU; 234094; -.
DR Ensembl; ENSMUST00000084207.12; ENSMUSP00000081225.6; ENSMUSG00000071176.11. [Q8C033-1]
DR Ensembl; ENSMUST00000110800.9; ENSMUSP00000106424.3; ENSMUSG00000071176.11. [Q8C033-2]
DR GeneID; 234094; -.
DR KEGG; mmu:234094; -.
DR UCSC; uc009kzg.2; mouse. [Q8C033-1]
DR UCSC; uc009kzh.2; mouse. [Q8C033-2]
DR AGR; MGI:2444453; -.
DR CTD; 9639; -.
DR MGI; MGI:2444453; Arhgef10.
DR VEuPathDB; HostDB:ENSMUSG00000071176; -.
DR eggNOG; KOG3522; Eukaryota.
DR GeneTree; ENSGT00940000153798; -.
DR InParanoid; Q8C033; -.
DR OMA; NSDSEPX; -.
DR OrthoDB; 2916834at2759; -.
DR PhylomeDB; Q8C033; -.
DR TreeFam; TF331430; -.
DR Reactome; R-MMU-193648; NRAGE signals death through JNK.
DR Reactome; R-MMU-416482; G alpha (12/13) signalling events.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR Reactome; R-MMU-9013026; RHOB GTPase cycle.
DR Reactome; R-MMU-9013106; RHOC GTPase cycle.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR Reactome; R-MMU-9013149; RAC1 GTPase cycle.
DR BioGRID-ORCS; 234094; 2 hits in 77 CRISPR screens.
DR ChiTaRS; Arhgef10; mouse.
DR PRO; PR:Q8C033; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8C033; Protein.
DR Bgee; ENSMUSG00000071176; Expressed in sciatic nerve and 229 other cell types or tissues.
DR ExpressionAtlas; Q8C033; baseline and differential.
DR Genevisible; Q8C033; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISO:MGI.
DR GO; GO:0019894; F:kinesin binding; ISO:MGI.
DR GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0051298; P:centrosome duplication; ISO:MGI.
DR GO; GO:0090307; P:mitotic spindle assembly; ISO:MGI.
DR GO; GO:0022011; P:myelination in peripheral nervous system; ISO:MGI.
DR GO; GO:0051496; P:positive regulation of stress fiber assembly; ISO:MGI.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR039919; ARHGEF10/ARHGEF17.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR12877; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR12877:SF14; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 10; 1.
DR Pfam; PF19057; PH_19; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF19056; WD40_2; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50010; DH_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Guanine-nucleotide releasing factor;
KW Methylation; Phosphoprotein; Reference proteome.
FT CHAIN 1..1345
FT /note="Rho guanine nucleotide exchange factor 10"
FT /id="PRO_0000080927"
FT DOMAIN 397..584
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT REGION 1..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 99..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 207..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1202..1237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1253..1306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 307..335
FT /evidence="ECO:0000255"
FT COMPBIAS 36..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1202..1224
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1253..1277
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 157
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 355
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O15013"
FT MOD_RES 1262
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1314
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250|UniProtKB:O15013"
FT VAR_SEQ 283..321
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.2"
FT /id="VSP_010707"
FT CONFLICT 184
FT /note="Missing (in Ref. 1; BAC35629)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="R -> Q (in Ref. 1; BAC27875)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="M -> I (in Ref. 2; BAD90400)"
FT /evidence="ECO:0000305"
FT CONFLICT 348
FT /note="G -> R (in Ref. 2; BAD90400)"
FT /evidence="ECO:0000305"
FT CONFLICT 528
FT /note="D -> Y (in Ref. 1; BAC27875)"
FT /evidence="ECO:0000305"
FT CONFLICT 581
FT /note="L -> R (in Ref. 1; BAC35629)"
FT /evidence="ECO:0000305"
FT CONFLICT 1332
FT /note="V -> F (in Ref. 3; AAH06842)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1345 AA; 147946 MW; 8583299B5A4C5835 CRC64;
MEQGEASPPV PAEHEAKCDT SNNEEEGELF DFDSGDEVPE ADRQVPSADD RTRGAEAGGA
DENTCPAGNG TGAEPAPEAE PAKLVVPTKV NPYSVIDITP LQEDQPSSPD ANTEEEGVGL
RVPSGYSVPV PCGYAVPSNL PLLLPAYSSP VIIRAESVEE EEAAETVGDG QCNSLSSEDL
PHSSEQGSQE GSALARWAAD PANTAWMENP EEAIYDDVPR ENSDSEPDEM IYDDVENGEE
GGNSSPEYGW SSSEFESYEE PSDSEGKNGI PRSFLRSSHK KQLSHDLTRF KAHCEEKMRG
LVASTVGAME IQQAKQRQER KMQKLMKAAK EGTKDGLEKT KAAVKRGGSF IRTRSLVSQD
HRCYFEEEQN LFIDVDCKHP EAVLTPMPEG LSQQQVVRRY ILGSIVESEK NYVDALRRIL
EQYEKPLSEM EPRLLSDRKL RMVFYRVKEI LQCHSMFQIA LASRVSEWDV VETIGDVFVA
SFSKSMVLDA YSEYVNNFST AVAVLKKTCA TKPAFLEFLK LSQDSSPDRV TLHSLMMRPI
QRFPQFILLL QDMLKNTAKG HPDRLPLQMA LTELETLAEK LNERKRDADQ RCEIKQIAKA
INERYLNKLL SSGNRYLVRS DDVIETVYND RGEIVKTKQR RIFMLNDVLM CATASSRNSH
ESHAVMSQRY LLKWSVPLGH VDVIQYNGGS GAGEHCRHHA AHSPESLAVV ANAKPHKVYM
GPGQLYQDLQ NLLHDLNVVG QISQLIGNLR GSYQNLNQSV AHDWTSGLQR LILRKEDAIR
AADRCRIQLQ LPGKQDKSGR PTFFTAVFNT LTPAIKESWV SSLQMAKLAL EEENHMGWFC
VDDDGNLAKK ETHPLLVGHM PVMVAKQPEF KIECAAYNPE PYLSNESQPA SPSTARGFLW
IGSCSNQMGQ VAIVSFQGSN PKVIECFNVE SRILCMVYIP AEESKPQETT ETKDPEATAS
RAPHVPTICL GTEEGSISIY KSSQGCKKVR LQHFYAPDKS TVMSLACSPQ GLYAGLVNGS
VASYTKAPDG SWNSEPQQVI KLGVLPVRSL LLVEGALWAA SGGQVFMASV ETHTIENQLE
AHQDEGMVIS HMAVAGVGIW IAFTSGSTLR LFHTETLKHL QDVNIDAPVH SMLPGHQRLS
VTSLLVCHGL LMVGTSLGVV VALPVPRLQG IPKVTGRGMV SYHAHNGPVK FIVSATAFQN
KDRARDSPRS GSELQDEDPK DLLCSEEGPS CPGQPDTYTS VWLGDSLGLP TQKNDLSSSS
GSLNLSHGSS SLEHRSVDSS LCDLLRDPSA SPRSRPQGSR RARASSALVV CGGQGHRRVH
RKARQPSQED LVSSVMVWQI PLLGM
//
