ID B0K7P9_THEP3 Unreviewed; 358 AA.
AC B0K7P9;
DT 18-MAR-2008, integrated into UniProtKB/TrEMBL.
DT 18-MAR-2008, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=N(4)-bis(aminopropyl)spermidine synthase {ECO:0000256|HAMAP-Rule:MF_01947};
DE EC=2.5.1.128 {ECO:0000256|HAMAP-Rule:MF_01947};
DE AltName: Full=Branched-chain polyamine synthase A {ECO:0000256|HAMAP-Rule:MF_01947};
GN Name=bpsA {ECO:0000256|HAMAP-Rule:MF_01947};
GN OrderedLocusNames=Teth39_0634 {ECO:0000313|EMBL:ABY94298.1};
OS Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium
OS thermohydrosulfuricum).
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Thermoanaerobacter.
OX NCBI_TaxID=340099 {ECO:0000313|EMBL:ABY94298.1, ECO:0000313|Proteomes:UP000002156};
RN [1] {ECO:0000313|Proteomes:UP000002156}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33223 / DSM 2355 / 39E
RC {ECO:0000313|Proteomes:UP000002156};
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T.,
RA Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J.,
RA Richardson P.;
RT "Complete sequence of Thermoanaerobacter pseudethanolicus 39E.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of branched-chain polyamines,
CC which support the growth of thermophiles under high-temperature
CC conditions. Catalyzes the sequential condensation of spermidine with
CC the aminopropyl groups of decarboxylated S-adenosylmethionines to
CC produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine.
CC {ECO:0000256|HAMAP-Rule:MF_01947}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2
CC H(+) + N(4)-bis(aminopropyl)spermidine + 2 S-methyl-5'-thioadenosine;
CC Xref=Rhea:RHEA:44132, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509,
CC ChEBI:CHEBI:57443, ChEBI:CHEBI:57834, ChEBI:CHEBI:82771;
CC EC=2.5.1.128; Evidence={ECO:0000256|HAMAP-Rule:MF_01947};
CC -!- PATHWAY: Amine and polyamine biosynthesis. {ECO:0000256|HAMAP-
CC Rule:MF_01947}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01947}.
CC -!- SIMILARITY: Belongs to the branched-chain polyamine synthase family.
CC {ECO:0000256|HAMAP-Rule:MF_01947}.
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DR EMBL; CP000924; ABY94298.1; -; Genomic_DNA.
DR RefSeq; WP_004403525.1; NC_010321.1.
DR AlphaFoldDB; B0K7P9; -.
DR STRING; 340099.Teth39_0634; -.
DR KEGG; tpd:Teth39_0634; -.
DR eggNOG; COG1568; Bacteria.
DR HOGENOM; CLU_042160_0_0_9; -.
DR Proteomes; UP000002156; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IEA:UniProtKB-UniRule.
DR GO; GO:0006596; P:polyamine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR HAMAP; MF_01947; Aminopropyltransf_BpsA; 1.
DR InterPro; IPR014435; BpsA.
DR InterPro; IPR002723; BpsA_C.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23290:SF0; RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5; 1.
DR PANTHER; PTHR23290; UNCHARACTERIZED; 1.
DR Pfam; PF01861; BpsA_C; 1.
DR PIRSF; PIRSF005895; UCP005895_mtase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01947};
KW Polyamine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01947};
KW Reference proteome {ECO:0000313|Proteomes:UP000002156};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_01947}.
FT DOMAIN 110..350
FT /note="N(4)-bis(aminopropyl)spermidine synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01861"
SQ SEQUENCE 358 AA; 40689 MW; 6DD359DC14ABC40D CRC64;
MESLQDIAHQ IYQKTGVMTS SKDVEKILSA AAATPHFWEI ISLSQKPFSV VAEIIELLRQ
RNLLKVEKNG DIKFNSKGLE YLGSLKIAPK RTYTCHACEG RGINFSKLED LIKKFDQVTV
NRPKAISDYD QGFVTTQTVV GRIALLAERG DLEGKKLLVL GDDDLVSIAA GLSGMPTEIV
VMEIDDRLVE YINEVANKYN LPIKAMKYDF RDKLPEEYVG YFDTFITDPP ETIEALEICI
GRGISALSKE GCAGYFGLTL IEASLEKWNV FQQILASKFK VVVTDIIHNF NHYVNWDYLL
NTVGKQYEFV QVEPKLNWYR SSMYRIETLK DSKGIENEYK PCELYVDEEA LIYRGDKA
//
