ID B0WN53_CULQU Unreviewed; 375 AA.
AC B0WN53;
DT 08-APR-2008, integrated into UniProtKB/TrEMBL.
DT 08-APR-2008, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE SubName: Full=Heparan sulfate glucosamine 3-O-sulfotransferase 3B1 {ECO:0000313|EMBL:EDS31484.1, ECO:0000313|EnsemblMetazoa:CPIJ008491-PA};
GN Name=6040796 {ECO:0000313|EnsemblMetazoa:CPIJ008491-PA};
GN ORFNames=CpipJ_CPIJ008491 {ECO:0000313|EMBL:EDS31484.1};
OS Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Culicini; Culex; Culex.
OX NCBI_TaxID=7176;
RN [1] {ECO:0000313|EMBL:EDS31484.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JHB {ECO:0000313|EMBL:EDS31484.1};
RG The Broad Institute Genome Sequencing Platform;
RA Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.,
RA Hannick L., Megy K., O'Leary S., Pearson M., Haas B.J., Mauceli E.,
RA Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA Fraser-Liggett C., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT "Annotation of Culex pipiens quinquefasciatus.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:CPIJ008491-PA}
RP IDENTIFICATION.
RC STRAIN=JHB {ECO:0000313|EnsemblMetazoa:CPIJ008491-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DS232007; EDS31484.1; -; Genomic_DNA.
DR RefSeq; XP_001850137.1; XM_001850085.1.
DR AlphaFoldDB; B0WN53; -.
DR STRING; 7176.B0WN53; -.
DR EnsemblMetazoa; CPIJ008491-RA; CPIJ008491-PA; CPIJ008491.
DR KEGG; cqu:CpipJ_CPIJ008491; -.
DR VEuPathDB; VectorBase:CPIJ008491; -.
DR VEuPathDB; VectorBase:CQUJHB013954; -.
DR eggNOG; KOG3704; Eukaryota.
DR HOGENOM; CLU_017703_0_1_1; -.
DR InParanoid; B0WN53; -.
DR OMA; RSHPYID; -.
DR Proteomes; UP000002320; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008146; F:sulfotransferase activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR037359; NST/OST.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR PANTHER; PTHR10605:SF73; HEPARAN SULFATE 3-O SULFOTRANSFERASE-B, ISOFORM A; 1.
DR PANTHER; PTHR10605; HEPARAN SULFATE SULFOTRANSFERASE; 1.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 4: Predicted;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR637359-3};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002320};
KW Transferase {ECO:0000313|EMBL:EDS31484.1};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..31
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 122..358
FT /note="Sulfotransferase"
FT /evidence="ECO:0000259|Pfam:PF00685"
FT ACT_SITE 131
FT /note="For sulfotransferase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-1"
FT BINDING 131..135
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 212
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 220
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 338..342
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT DISULFID 321..333
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-3"
SQ SEQUENCE 375 AA; 42832 MW; D3D971CEF58D5AEB CRC64;
MVYKWYANLS NRTIAITIVV CICMLYVSYT FNTCLISSIN KTWKKVCGGK APQAHTSITT
MTTPTEETEA PQIKILGSIV RIVPLTDDSS GFEAKGMLNA SDGSPKYRFL RQQGLRPSRH
LPDALIIGVK KSGTRALLEF IRLHPDVRAA GCEVHFFDRH YAKGLHWYRH HMPPTIEGQI
TMEKTPSYFI TKEAPKRVYH MNPSTKLLVV VRDPVTRAIS DYTQARSKKK DMKRFEELAF
LNGTSGGVVD TNWGPVKIGV YAKHLERWLE YFPLSQLIFV SGERLIADPA VEIGRVQDFL
GLKRVVNEKH FYFNSTKGFP CLLKSEERSS PHCLGKTKGR NHPKIESQAI ERLREFYRPF
NLKFYQLTGI NFGWP
//
