ID B1M9K4_METRJ Unreviewed; 442 AA.
AC B1M9K4;
DT 29-APR-2008, integrated into UniProtKB/TrEMBL.
DT 29-APR-2008, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN OrderedLocusNames=Mrad2831_6255 {ECO:0000313|EMBL:ACB28179.1};
OS Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 /
OS NBRC 15690 / NCIMB 10815 / 0-1).
OG Plasmid pMRAD01 {ECO:0000313|EMBL:ACB28179.1,
OG ECO:0000313|Proteomes:UP000006589}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=426355 {ECO:0000313|EMBL:ACB28179.1, ECO:0000313|Proteomes:UP000006589};
RN [1] {ECO:0000313|EMBL:ACB28179.1, ECO:0000313|Proteomes:UP000006589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 /
RC 0-1 {ECO:0000313|Proteomes:UP000006589};
RC PLASMID=Plasmid pMRAD01 {ECO:0000313|Proteomes:UP000006589};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Marx C.J., Richardson P.;
RT "Complete sequence of plasmid1 of Methylobacterium radiotolerans JCM
RT 2831.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC {ECO:0000256|ARBA:ARBA00010945}.
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DR EMBL; CP001002; ACB28179.1; -; Genomic_DNA.
DR RefSeq; WP_012329965.1; NC_010510.1.
DR AlphaFoldDB; B1M9K4; -.
DR KEGG; mrd:Mrad2831_6255; -.
DR PATRIC; fig|426355.14.peg.6333; -.
DR eggNOG; COG0389; Bacteria.
DR HOGENOM; CLU_012348_3_0_5; -.
DR OrthoDB; 9808813at2; -.
DR Proteomes; UP000006589; Plasmid pMRAD01.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR CDD; cd01700; PolY_Pol_V_umuC; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.1490.100; DNA polymerase, Y-family, little finger domain; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR036775; DNA_pol_Y-fam_lit_finger_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR025188; DUF4113.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR11076; DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER; 1.
DR PANTHER; PTHR11076:SF34; PROTEIN UMUC; 1.
DR Pfam; PF13438; DUF4113; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS50173; UMUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00023199};
KW DNA-directed DNA polymerase {ECO:0000313|EMBL:ACB28179.1};
KW Nucleotidyltransferase {ECO:0000313|EMBL:ACB28179.1};
KW Plasmid {ECO:0000313|EMBL:ACB28179.1};
KW SOS mutagenesis {ECO:0000256|ARBA:ARBA00023199};
KW SOS response {ECO:0000256|ARBA:ARBA00023236};
KW Transferase {ECO:0000313|EMBL:ACB28179.1}.
FT DOMAIN 18..202
FT /note="UmuC"
FT /evidence="ECO:0000259|PROSITE:PS50173"
SQ SEQUENCE 442 AA; 48574 MW; BBCC6A56AF266DD3 CRC64;
MSTPEARRRD RIGGGRAVAL IDGNSFYCSC ERVFDPKLAT VPVIVLSNND GCAIARTSEA
KALGIKMGDP YFKIREMCRA QGVRVFSSNY TLYGDMSGRT NAVYRQFSPQ VEIYSIDESF
LNLSDVAPAL RVELARDLRA TVRAWTGIPT CVGIGPTKTL AKLANHIAKS VPVLGGVCDL
TDPAAYAHWL CRIDVAEVWG IGRASLAKLQ AMGVESVADL RDLDPRPVRK GLTVVGERII
YELRGAACLP LELVPARRKG CAVTRSFSSR ITERAVLEEA VAAHATRLGE KLRREGLGTN
HVTLFYHTSE HDRDEPMRSV STTVQLPEHS SDTLALIKAA RHGVARTWRE PGERPWRYSK
AGIVTNDLVP LSHSPRALIG ALDRERSGPL MVAIDACNAR WGRGAVVPAR AGLVNRRDWT
TRFEMRTPRY TTRVGELPVA HA
//
