ID B2J4H2_NOSP7 Unreviewed; 684 AA.
AC B2J4H2;
DT 10-JUN-2008, integrated into UniProtKB/TrEMBL.
DT 10-JUN-2008, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Npun_F5347 {ECO:0000313|EMBL:ACC83662.1};
OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=63737 {ECO:0000313|EMBL:ACC83662.1, ECO:0000313|Proteomes:UP000001191};
RN [1] {ECO:0000313|Proteomes:UP000001191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29133 / PCC 73102 {ECO:0000313|Proteomes:UP000001191};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACC83662.1, ECO:0000313|Proteomes:UP000001191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29133 / PCC 73102 {ECO:0000313|Proteomes:UP000001191};
RX PubMed=23463784; DOI=10.1104/pp.112.213116;
RA Ekman M., Picossi S., Campbell E.L., Meeks J.C., Flores E.;
RT "A Nostoc punctiforme Sugar Transporter Necessary to Establish a
RT Cyanobacterium-Plant Symbiosis.";
RL Plant Physiol. 161:1984-1992(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP001037; ACC83662.1; -; Genomic_DNA.
DR RefSeq; WP_012411613.1; NC_010628.1.
DR AlphaFoldDB; B2J4H2; -.
DR STRING; 63737.Npun_F5347; -.
DR EnsemblBacteria; ACC83662; ACC83662; Npun_F5347.
DR KEGG; npu:Npun_F5347; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_012122_0_0_3; -.
DR OrthoDB; 467945at2; -.
DR PhylomeDB; B2J4H2; -.
DR Proteomes; UP000001191; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ACC83662.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001191};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 173..447
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 684 AA; 78094 MW; 849320ACF8A6CEEE CRC64;
MTVNNILQYE SNIWATADLL RGCGIKESEW PSYMMPFFAL VMIESRLVRM FDELKAEIGE
AALAEIAPED LTGLIEDKGQ GYNVYIFEKN QTLKDICKND KSFDVDFEAY LRGFDGETKD
LLGVEATEGE KFLDIKGVIT KLKAKKVLLG YTKEWSSIDL KPFDNSAITT LEEHIKRRWA
DISADTAGEQ YTPDDVIGLI AEIIASKIEE SDKLLKIYDC TCGGGNLLFG VEDRIHQRFK
RLTQTFGQDW NDALYALAKI ESRFRVDSKI EHGNTLTDDK FYNDEFDVVI ANPPYGVKWN
GYQKDIENDK TQRFKYLPSI SDGQLLFMQH LISKLNANGM GVVVHNGSTL FSGDAGSAES
NIRKWMLDSD FVEAVIQLPT DEFFNTGIYT YLWVLNKHKL PQCRDKVMLI NASEKFKPLK
KSKGSKRKEV DEVSRLEIVE TLTRFVDNDY ARVFDKEFFY FNKQAIMLTN VDEQGKSFAS
RLKEGKISLK LSPLKLDNGE RTLTEFTITN CDSQRFGSLV EAFEQDIKPF VSSLDYKEQP
LTVTTEKALY RFDADRETLI KEVLGKQEEA LGCGKIVVKA AFKKGTKTQP EKIEITVELT
PDYQKDYEII PFHRDEVANQ EAIEAFMAKY ITKPFEYLEN VVGVEINFNK VFYKPEKLRS
VQKILGEITA IEKELKGLEE GLGL
//
