ID B3G164_PSEAI Unreviewed; 324 AA.
AC B3G164;
DT 22-JUL-2008, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2008, sequence version 1.
DT 13-SEP-2023, entry version 61.
DE RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000256|HAMAP-Rule:MF_01470};
DE EC=3.1.-.- {ECO:0000256|HAMAP-Rule:MF_01470};
GN Name=cas1 {ECO:0000256|HAMAP-Rule:MF_01470};
GN ORFNames=PACL_0131 {ECO:0000313|EMBL:ACD38776.1};
OS Pseudomonas aeruginosa.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=287 {ECO:0000313|EMBL:ACD38776.1};
RN [1] {ECO:0000313|EMBL:ACD38776.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PACS458 {ECO:0000313|EMBL:ACD38776.1};
RX PubMed=18445516; DOI=10.1016/j.ygeno.2008.02.005;
RA Hayden H.S., Gillett W., Saenphimmachak C., Lim R., Zhou Y., Jacobs M.A.,
RA Chang J., Rohmer L., D'Argenio D.A., Palmieri A., Levy R., Haugen E.,
RA Wong G.K., Brittnacher M.J., Burns J.L., Miller S.I., Olson M.V., Kaul R.;
RT "Large-insert genome analysis technology detects structural variation in
RT Pseudomonas aeruginosa clinical strains from cystic fibrosis patients.";
RL Genomics 91:530-537(2008).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC the CRISPR cassette. {ECO:0000256|HAMAP-Rule:MF_01470}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01470};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01470};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC {ECO:0000256|HAMAP-Rule:MF_01470}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC {ECO:0000256|HAMAP-Rule:MF_01470}.
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DR EMBL; EU595739; ACD38776.1; -; Genomic_DNA.
DR RefSeq; WP_004349917.1; NZ_WTLJ01000083.1.
DR AlphaFoldDB; B3G164; -.
DR PATRIC; fig|287.1479.peg.6051; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004520; F:DNA endonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR CDD; cd09718; Cas1_I-F; 1.
DR Gene3D; 1.20.120.920; CRISPR-associated endonuclease Cas1, C-terminal domain; 1.
DR Gene3D; 3.100.10.20; CRISPR-associated endonuclease Cas1, N-terminal domain; 1.
DR HAMAP; MF_01470; Cas1; 1.
DR InterPro; IPR002729; CRISPR-assoc_Cas1.
DR InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR InterPro; IPR019857; CRISPR-assoc_Cas1_YPEST-subtyp.
DR NCBIfam; TIGR00287; cas1; 1.
DR NCBIfam; TIGR03637; cas1_YPEST; 1.
DR PANTHER; PTHR34353:SF3; CRISPR-ASSOCIATED ENDONUCLEASE CAS1; 1.
DR PANTHER; PTHR34353; CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1; 1.
DR Pfam; PF01867; Cas_Cas1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|HAMAP-Rule:MF_01470};
KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_01470};
KW Endonuclease {ECO:0000256|HAMAP-Rule:MF_01470};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_01470};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_01470};
KW Manganese {ECO:0000256|HAMAP-Rule:MF_01470};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_01470};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_01470}.
FT BINDING 190
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01470"
FT BINDING 254
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01470"
FT BINDING 268
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01470"
SQ SEQUENCE 324 AA; 36107 MW; 24D3902F830E972E CRC64;
MDDISPSELK TILHSKRANL YYLQHCRVLV NGGRVEYVTD EGRHSHYWNI PIANTTSLLL
GTGTSITQAA MRELARAGVL VGFCGGGGTP LFSANEVDVE VSWLTPQSEY RPTEYLQLWV
GFWFDEEKRL EAARHFQRVR LERIRHSWLE DRVLRDAGFA VDATALAVAV EDSARALEQA
PNHEHLLTEE ARLSKRLFKL AAQATRYGEF VRAKRGSGGD PANRFLDHGN YLAYGLAATA
TWVLGIPHGL AVLHGKTRRG GLVFDVADLV KDSLILPQAF LSAMRGDEEQ DFRQACLDNL
SRAQALDFMI DTLKDVAQRS TVSA
//
