ID WUHO_DROAN Reviewed; 407 AA.
AC B3MRC6;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GF21256;
OS Drosophila ananassae (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7217;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 14024-0371.13;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC induce conformational changes of the catalytic subunit. Required during
CC gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC at early stages, from germarium to stage 4 egg chambers. Also present
CC in the nuclei of spermatocytes and in the apical cells of the testes.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
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DR EMBL; CH902622; EDV34331.1; -; Genomic_DNA.
DR RefSeq; XP_001963882.1; XM_001963846.2.
DR AlphaFoldDB; B3MRC6; -.
DR SMR; B3MRC6; -.
DR STRING; 7217.B3MRC6; -.
DR EnsemblMetazoa; FBtr0125956; FBpp0124448; FBgn0098258.
DR GeneID; 6503940; -.
DR KEGG; dan:6503940; -.
DR eggNOG; KOG3914; Eukaryota.
DR HOGENOM; CLU_054270_0_0_1; -.
DR InParanoid; B3MRC6; -.
DR OMA; SVWFKKR; -.
DR OrthoDB; 5480021at2759; -.
DR PhylomeDB; B3MRC6; -.
DR UniPathway; UPA00989; -.
DR Proteomes; UP000007801; Unassembled WGS sequence.
DR GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR011044; Quino_amine_DH_bsu.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR16288:SF0; TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4; 1.
DR PANTHER; PTHR16288; WD40 REPEAT PROTEIN 4; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50969; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; Nucleus; Oogenesis;
KW Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT CHAIN 1..407
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit wuho"
FT /id="PRO_0000370542"
FT REPEAT 83..124
FT /note="WD 1"
FT REPEAT 171..210
FT /note="WD 2"
FT REPEAT 214..252
FT /note="WD 3"
SQ SEQUENCE 407 AA; 45296 MW; 4A14E92870E95966 CRC64;
MATIFFADPE LVIGHGRRVL FVNPDDLQLF KEIELPPDLA TCGLKVADSK EQHCQHEQQD
VAAAGSAKQS SNTASASASK AKAIEVSIQN VSYSPDRQLL AITTTGGQKA LLLYRSRMEH
ARLLSVRPLA RASSALRFCS DGSSVLVTDK TGDCYQYDCV EVEAAPRLLL GHLSVVFDIL
WTDDQQHIIT CDRDDKIRVT NYPATFDIHS YCLGHKEFVS GLALLTEQHI VSSSGDKTLR
VWNFIEGKEL LIHQLPAPAV RLQVQQLEPE KVYQVAVLFY DHVDGVAIYR LERSSGDNWA
ITATTVVRAD AGLWNICNFT LTGNRVYVTG AENECLAIKA YDIGSGETST SVPDGWVKMV
LRGLEAEGVA YLPEDLSVWF KKRFDNVSDY LERKKRRIEE QQQQKCG
//
