ID B4CWW4_9BACT Unreviewed; 380 AA.
AC B4CWW4;
DT 23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 23-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=Thiazole biosynthesis protein ThiH {ECO:0000313|EMBL:EDY21284.1};
GN ORFNames=CfE428DRAFT_1577 {ECO:0000313|EMBL:EDY21284.1};
OS Chthoniobacter flavus Ellin428.
OC Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales;
OC Chthoniobacteraceae; Chthoniobacter.
OX NCBI_TaxID=497964 {ECO:0000313|EMBL:EDY21284.1, ECO:0000313|Proteomes:UP000005824};
RN [1] {ECO:0000313|EMBL:EDY21284.1, ECO:0000313|Proteomes:UP000005824}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ellin428 {ECO:0000313|EMBL:EDY21284.1,
RC ECO:0000313|Proteomes:UP000005824};
RX PubMed=21460085; DOI=10.1128/JB.00295-11;
RA Kant R., van Passel M.W., Palva A., Lucas S., Lapidus A.,
RA Glavina Del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S.,
RA Larimer F.W., Land M.L., Hauser L., Sangwan P., de Vos W.M., Janssen P.H.,
RA Smidt H.;
RT "Genome sequence of Chthoniobacter flavus Ellin428, an aerobic
RT heterotrophic soil bacterium.";
RL J. Bacteriol. 193:2902-2903(2011).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDY21284.1}.
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DR EMBL; ABVL01000003; EDY21284.1; -; Genomic_DNA.
DR RefSeq; WP_006978903.1; NZ_ABVL01000003.1.
DR AlphaFoldDB; B4CWW4; -.
DR STRING; 497964.CfE428DRAFT_1577; -.
DR GeneID; 78186796; -.
DR eggNOG; COG0502; Bacteria.
DR InParanoid; B4CWW4; -.
DR Proteomes; UP000005824; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR012726; ThiH.
DR InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR NCBIfam; TIGR02351; thiH; 1.
DR PANTHER; PTHR43583; 2-IMINOACETATE SYNTHASE; 1.
DR PANTHER; PTHR43583:SF1; 2-IMINOACETATE SYNTHASE; 1.
DR Pfam; PF06968; BATS; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR SFLD; SFLDG01081; cleavage_of_the_Ca-Cb_bond_in; 1.
DR SMART; SM00876; BATS; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000005824};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 47..276
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 380 AA; 41578 MW; D12A3AD0C68995D8 CRC64;
MTPAAPHSAP ASPVLRRFAA LLEPKTDAQL ETLARESAAL TRQHFGKTIR LFAPLYLSNE
CINNCAYCGF SRDNAILRVT LDVQSVVKEA RHLAKEGFRN ILLVAGEHPK FVSNGYLEHC
VRALVAEIPS ISLEVAPMET PEYAALAGAG AEGLVVYQET YDREVYAAMH TAGPKKDYDW
RLACPERAYA GGFRRIGVGA LLGLAEWRTE ARALAAHLEH LLRTCWKAQF TVSLPRLRPC
AGEFTPTHPV ADREFIQLIC ALRLCFPQVG IVLSTREPAP LRDALLPLGI TMMSAGSHTE
PGGYTGQGRN DLHLTVRGKI MAPVDSEAEG ATGQFEISDT RSPREVADFI RARGFEPVWK
DWDRAILDAA AHSAEVVAVA
//
