ID WUHO_DROGR Reviewed; 385 AA.
AC B4JWS7;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GH17702;
OS Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Hawaiian Drosophila.
OX NCBI_TaxID=7222;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 15287-2541.00;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC induce conformational changes of the catalytic subunit. Required during
CC gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC at early stages, from germarium to stage 4 egg chambers. Also present
CC in the nuclei of spermatocytes and in the apical cells of the testes.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
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DR EMBL; CH916376; EDV95203.1; -; Genomic_DNA.
DR RefSeq; XP_001995551.1; XM_001995515.1.
DR AlphaFoldDB; B4JWS7; -.
DR SMR; B4JWS7; -.
DR STRING; 7222.B4JWS7; -.
DR EnsemblMetazoa; FBtr0153116; FBpp0151608; FBgn0125172.
DR GeneID; 6569215; -.
DR KEGG; dgr:6569215; -.
DR eggNOG; KOG3914; Eukaryota.
DR HOGENOM; CLU_054270_0_0_1; -.
DR InParanoid; B4JWS7; -.
DR OMA; SVWFKKR; -.
DR OrthoDB; 5480021at2759; -.
DR PhylomeDB; B4JWS7; -.
DR UniPathway; UPA00989; -.
DR Proteomes; UP000001070; Unassembled WGS sequence.
DR GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR16288:SF0; TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4; 1.
DR PANTHER; PTHR16288; WD40 REPEAT PROTEIN 4; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50960; TolB, C-terminal domain; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; Nucleus; Oogenesis;
KW Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT CHAIN 1..385
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit wuho"
FT /id="PRO_0000370544"
FT REPEAT 68..108
FT /note="WD 1"
FT REPEAT 155..194
FT /note="WD 2"
FT REPEAT 198..236
FT /note="WD 3"
SQ SEQUENCE 385 AA; 43183 MW; 2DE4681B59FC6032 CRC64;
MTTIFYAEPE LVIAYGRKVL FLNPNDLQIF KDIELPADLT SCGLKITAPE ISEEQQSTSE
GKGTDGGKVE VAILNVCYSP DRQLIALTTA GQKALLLYKS RPEHAKLLSV RALARASSAM
TFAADSSSVL VTDKTGDCYQ YDCVEVNALP RLLLGHLSIV YDILWTPDQK HIITSDRDDK
IRVTNYPATF DIHSYCLGHK EFVSGLALLT EEHLLSVSGD KTLRLWNYLS GMELLHHDLP
APAVRLHMRE LLPAKRYQAA VQFYDHIEAI GLYELEQTNN IWTVACEQLV RAEAGSWNIS
NFALTSDMIY VAGAVNEHLA LRVYNASDGE QAKTVPAGWL DMVTGSFSDQ TCVPEDLSVW
FKKRYDNVSD YMERKKRRIE GQQQK
//
