ID B5U173_9SOLN Unreviewed; 270 AA.
AC B5U173;
DT 04-NOV-2008, integrated into UniProtKB/TrEMBL.
DT 04-NOV-2008, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE SubName: Full=Nitrate reductase {ECO:0000313|EMBL:ACI01559.1};
DE EC=1.7.1.1 {ECO:0000313|EMBL:ACI01559.1};
DE Flags: Fragment;
GN Name=NIA {ECO:0000313|EMBL:ACI01559.1};
OS Solanum berthaultii.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=47970 {ECO:0000313|EMBL:ACI01559.1};
RN [1] {ECO:0000313|EMBL:ACI01559.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:ACI01559.1};
RA Rodriguez F., Spooner D.M.;
RT "Nitrate Reductase Phylogeny of Potato (Solatium sect. Petota) Genomes with
RT Emphasis on the Origins of the Polyploid Species.";
RL Syst. Bot. 34:207-219(2009).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=Mo-molybdopterin; Xref=ChEBI:CHEBI:71302;
CC Evidence={ECO:0000256|ARBA:ARBA00001924};
CC -!- SIMILARITY: Belongs to the cytochrome b5 family.
CC {ECO:0000256|RuleBase:RU362121}.
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DR EMBL; FJ155394; ACI01559.1; -; Genomic_DNA.
DR AlphaFoldDB; B5U173; -.
DR GO; GO:0020037; F:heme binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030151; F:molybdenum ion binding; IEA:InterPro.
DR GO; GO:0009703; F:nitrate reductase (NADH) activity; IEA:UniProtKB-EC.
DR Gene3D; 2.60.40.650; -; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR001834; CBR-like.
DR InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR018506; Cyt_B5_heme-BS.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR005066; MoCF_OxRdtse_dimer.
DR InterPro; IPR008335; Mopterin_OxRdtase_euk.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR19370; NADH-CYTOCHROME B5 REDUCTASE; 1.
DR PANTHER; PTHR19370:SF185; NADH-CYTOCHROME B5 REDUCTASE 2; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00970; FAD_binding_6; 1.
DR Pfam; PF03404; Mo-co_dimer; 1.
DR PRINTS; PR00363; CYTOCHROMEB5.
DR PRINTS; PR00407; EUMOPTERIN.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 3: Inferred from homology;
KW Heme {ECO:0000256|ARBA:ARBA00022617, ECO:0000256|RuleBase:RU362121};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU362121};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU362121}; Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:ACI01559.1}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..270
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002838402"
FT DOMAIN 113..188
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACI01559.1"
FT NON_TER 270
FT /evidence="ECO:0000313|EMBL:ACI01559.1"
SQ SEQUENCE 270 AA; 30230 MW; D2A5D61A3A26F7A9 CRC64;
KYWCWCFWSL EVEVLDLLSA KEIAVRAIDE THNTQPEKLI WNVMGMMNNC WFRVKMNVCK
PHKGEIGIVF EHPTQPGNQS GGWMAKERHL EISAVAPPTL KKSISTPFMN TASKMYSMSE
VRKHNSSDSA WIIVHGHIYD ASRFLKDHPG GVDSILINAG TDCTEEFDAI HSDKAKKLLE
DFRIGELITT GYTSDSSPNT SVHGSSSISS FLAPIKELVQ TPTRSVALIP REKIPCKLVD
KQSISHDVRK FKFALPSEDL VLGLPVGKHI
//
