ID B5ZPL2_RHILW Unreviewed; 350 AA.
AC B5ZPL2;
DT 25-NOV-2008, integrated into UniProtKB/TrEMBL.
DT 25-NOV-2008, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=3-hydroxyisobutyryl-CoA hydrolase {ECO:0000256|ARBA:ARBA00011915};
DE EC=3.1.2.4 {ECO:0000256|ARBA:ARBA00011915};
GN OrderedLocusNames=Rleg2_0229 {ECO:0000313|EMBL:ACI53529.1};
OS Rhizobium leguminosarum bv. trifolii (strain WSM2304).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=395492 {ECO:0000313|EMBL:ACI53529.1, ECO:0000313|Proteomes:UP000008330};
RN [1] {ECO:0000313|EMBL:ACI53529.1, ECO:0000313|Proteomes:UP000008330}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSM2304 {ECO:0000313|EMBL:ACI53529.1,
RC ECO:0000313|Proteomes:UP000008330};
RX PubMed=21304679; DOI=10.4056/sigs.44642;
RA Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K., Tiwari R.,
RA Malfatti S., Kiss H., Lapidus A., Copeland A., Nolan M., Land M.,
RA Ivanova N., Mavromatis K., Markowitz V., Kyrpides N., Melino V., Denton M.,
RA Yates R., Howieson J.;
RT "Complete genome sequence of Rhizobium leguminosarum bv trifolii strain
RT WSM2304, an effective microsymbiont of the South American clover Trifolium
RT polymorphum.";
RL Stand. Genomic Sci. 2:66-76(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-
CC methylpropanoate + CoA + H(+); Xref=Rhea:RHEA:20888,
CC ChEBI:CHEBI:11805, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57340; EC=3.1.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001709};
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DR EMBL; CP001191; ACI53529.1; -; Genomic_DNA.
DR RefSeq; WP_012556547.1; NC_011369.1.
DR AlphaFoldDB; B5ZPL2; -.
DR STRING; 395492.Rleg2_0229; -.
DR KEGG; rlt:Rleg2_0229; -.
DR eggNOG; COG1024; Bacteria.
DR HOGENOM; CLU_009834_22_1_5; -.
DR Proteomes; UP000008330; Chromosome.
DR GO; GO:0003860; F:3-hydroxyisobutyryl-CoA hydrolase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd06558; crotonase-like; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR045004; ECH_dom.
DR InterPro; IPR032259; HIBYL-CoA-H.
DR PANTHER; PTHR43176:SF3; 3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43176; 3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED; 1.
DR Pfam; PF16113; ECH_2; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Isomerase {ECO:0000313|EMBL:ACI53529.1}.
FT DOMAIN 15..341
FT /note="Enoyl-CoA hydratase/isomerase"
FT /evidence="ECO:0000259|Pfam:PF16113"
SQ SEQUENCE 350 AA; 36943 MW; E3329079C37E143A CRC64;
MQDGEVIIEQ RGTAGIIRLN RPRALNSLTL PMIRAITEAL DGFAADAAVA SVVVAGEGER
GFCAGGDIRA LHESAHAGDG LASAFWREEF RLNHQIAVYP KPYVALMDGI TMGGGVGLSS
HGRHRIVTER TRLAMPETGI GYVPDVGATW LLPKAPGEAG TWLGLTGLDI GAADAIHAGL
ADRQIAAARL GSVIEALSAL PRGSASSDVD ALLQGLAEPA GESRLKQNAA MIDRAFRFDS
VEEILAALAG EEGDFAAETR RVLSTRSPTS LKLALALLRA GRRSASLAEC LGRELGACLQ
MLDNPDFFEG IRAAVIDKDR NPKWSPASVE AVEPAMVERF LKPAEPPLSL
//
