ID B6JSA3_9INFA Unreviewed; 752 AA.
AC B6JSA3;
DT 16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT 16-DEC-2008, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ACI89870.1};
OS Influenza A virus (A/chicken/New York/Sg-00293/1996(H2N2)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=569215 {ECO:0000313|EMBL:ACI89870.1};
RN [1] {ECO:0000313|EMBL:ACI89870.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/chicken/New York/Sg-00293/1996 {ECO:0000313|EMBL:ACI89870.1};
RA Sreevatsan S., Wang P., Ramakrishnan M.A., Anderson T.L., Goyal S.M.,
RA Osterholm M.T.;
RT "unpublished.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; CY035571; ACI89870.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 281..478
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 47..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACI89870.1"
SQ SEQUENCE 752 AA; 85898 MW; 9B950CF4B04995B0 CRC64;
TLLFLKVPAQ NAISTTFPYT GDPPYSHGTG TGYTMDTVNR THQYSEKGKW TTNTETGAPQ
LNPIDGPLPE DNEPSGYAQT DCVLEAMAFL EESHPGIFEN SCLETMEIVQ QTRVDKLTQG
RQTYDWTLNR NQPAATALAN TIEVFRSNGL TANESGRLID FLKDVMESMD KEEMEITTHF
QRKRRVRDNM TKKMVTQRTI GKKKQRLNKR SYLIRALTLN TMTKDAERGK LKRRAIATPG
MQIRGFVYFV ETLARSICEK LEQSGLPVGG NEKKAKLANV VRKMMTNSQD TELSFTITGD
NTKWNENQNP RMFLAMITYI TRNQPEWFRN VLSIAPIMFS NKMARLGKGY MFESKSMKLR
TQIPAEMLXS IDLKYFNEST KKKIEKIRPL LIDGTASLSP GMMMGMFNML STVLGVSILN
LGQKRHTKTT YWWDGLQSSD DFALIVNAPN HEGIQAGVDR FYRTCKLVGI NMSKKKSYIN
RTGTFEFTSF FYRYGFVANF SMELPSFGVS GINESADMSI GVTVIKNNMI NNDLGPATAQ
MALQLFIKDY RYTYRCHRGD TQIQTRRSFE LKKLWEQTRS KAGLLVSDGG PNLYNIRNLH
IPEVCLKWEL MDEDYQGRLC NPLNPFVSHK EIESVNNAVV MPAHGPAKSM EYDAVATTHS
WIPKRNRSIL NTSQRGILED EQMYQKCCNL FEKFFPSSSY RRPVGISSMV EAMVSRARID
ARIDFESGRI KKEEFAEIMK ICSTIEELRR QR
//
