ID B6JVF4_SCHJY Unreviewed; 2390 AA.
AC B6JVF4;
DT 16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT 16-DEC-2008, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN Name=rad3 {ECO:0000313|JaponicusDB:SJAG_00364};
GN ORFNames=SJAG_00364 {ECO:0000313|EMBL:EEB05355.1};
OS Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=402676 {ECO:0000313|EMBL:EEB05355.1, ECO:0000313|Proteomes:UP000001744};
RN [1] {ECO:0000313|EMBL:EEB05355.1, ECO:0000313|Proteomes:UP000001744}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=yFS275 / FY16936 {ECO:0000313|Proteomes:UP000001744};
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000256|ARBA:ARBA00010769}.
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DR EMBL; KE651166; EEB05355.1; -; Genomic_DNA.
DR RefSeq; XP_002171648.1; XM_002171612.2.
DR STRING; 402676.B6JVF4; -.
DR EnsemblFungi; EEB05355; EEB05355; SJAG_00364.
DR GeneID; 7049511; -.
DR JaponicusDB; SJAG_00364; rad3.
DR VEuPathDB; FungiDB:SJAG_00364; -.
DR eggNOG; KOG0890; Eukaryota.
DR HOGENOM; CLU_000178_4_0_1; -.
DR OMA; SMYIGWC; -.
DR OrthoDB; 8448at2759; -.
DR Proteomes; UP000001744; Unassembled WGS sequence.
DR GO; GO:0070310; C:ATR-ATRIP complex; IEA:EnsemblFungi.
DR GO; GO:0000785; C:chromatin; IEA:EnsemblFungi.
DR GO; GO:0140445; C:chromosome, telomeric repeat region; IEA:EnsemblFungi.
DR GO; GO:0000228; C:nuclear chromosome; IEA:EnsemblFungi.
DR GO; GO:0005730; C:nucleolus; IEA:EnsemblFungi.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0033315; P:meiotic G2/MI DNA replication checkpoint signaling; IEA:EnsemblFungi.
DR GO; GO:0051598; P:meiotic recombination checkpoint signaling; IEA:EnsemblFungi.
DR GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IEA:EnsemblFungi.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IEA:EnsemblFungi.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IEA:EnsemblFungi.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:1904514; P:positive regulation of initiation of premeiotic DNA replication; IEA:EnsemblFungi.
DR GO; GO:0070198; P:protein localization to chromosome, telomeric region; IEA:EnsemblFungi.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00892; PIKKc_ATR; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR012993; UME.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF124; SERINE_THREONINE-PROTEIN KINASE MEC1; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF08064; UME; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM00802; UME; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00023204};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:EEB05355.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000001744};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1373..1944
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 2053..2374
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 2358..2390
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
SQ SEQUENCE 2390 AA; 273464 MW; 38C9FDA76BD98724 CRC64;
MNSNINTKAE SLTLSQQSFD DRNAFQQLLD EVLDVKKCSN SFADFSNAEV SLQLIQVLVE
AGLLPFLDKK KAENVRNSLF LRQAEQSLDA LLLVITAKPA LLNSTKSSIV FLEWLFPRTI
FLYSLESLIP IYDKLCGFHL QLLYLVNEHA QLSMHAVWLE EEWKRYIEYS FGALVSLSKS
QSVVPLYSAA PNFLMSNRTK KDDFLIALDT RSAATCFFLQ ASSVLAKYLP HSQLKPTDML
YIQHWDWLKQ HVFHLLLMQK LNEFEPAVQL SMYFRVLDVL VCFSLKYMLL KAPLCIRSRN
YILSISKAAF QLRESVKVDA EFTVSIMFVY FCAFQTLYPE HLTSAASEIM SLAVHYKEMT
EDAQTDARKC ANILMRCLKH KDSNFFHALQ AETDVSLENP FCKYFNNVIL LRFVRYFYST
LSDSPLGEFT NLKANQDVSS LYSIGKWVSN HNQAFEKRFE DELKSKVTSF LENTTELLVE
DLQLFATIHC SEKLNTSSKY DTKVHTHYCW LCDSPQRIKF STSAETEVLR KIPTEQKILD
AYWRWTNHSN TFIALADSDS GKRCLQMLFS SVRMLRMSAG RILPRFFSSF YTHEDIVSPT
ENRIMILETL KNLASGSHSK FLETTIMAWA HIAEVAEGEE LHLILLQLIE FIVEGDTFHQ
GVAVSCLHNV ARRRDVTIWQ LFSPYWRTVC VMIIKSVTHK PGIVKLFAET MGITDNDFLT
RTQAYTVPYL VLTKNRAVIQ KIAEASHNTV SSLCLSNMPK ILATFLTSES ANVEESVMLL
LTLASSDFEK IDLSRLLRSD PISVTVELLQ LCHKEAQVSQ VINAITKVAI LVSPSLNEKN
VNEETLLLDF FNNHILGILA EFSNIINNLR GKASINEKTR TIIGMKEMIQ FAGSATKLGL
PQITSCLQSA FQDNTLRSYA LEAWLALLLS IDGPEYLSLA SLSLVLLPSM YPKISKKEKI
IVVKVIDYIA KDPMDNIEQL RPVLPVSFAS ECFSETSRDS FSKIQVDDFH LNIEDIMQCC
RNEHESVCLL GLQKLASYLQ TKEAFLQSLI TVDVIDPLIN RLLRCLLDCC LKFAHSNDEI
SFLAAKNLGI LGAIDPNRAD AQKRIGGFLM LDNFENGEEC LKFITRFIQD QLIPAFITTT
DTKAQSFLAY ALQELLHLGG FRSAVIGSQK RSTKSVEAQY WNSLPDSSKR VLIPFLNSKY
HLTSSLKPRI SFPIYSETTT FTYWIQSFCL KLMQYYHSKN ADRIFSICAK VIKDQETSIS
RFLFPFLLLN AVLTESADEL KLISTELKMV VFQKRPERTN RESMQRYTTF LKCFFEVVDY
FNNWLRARRR RSWEERSAKA RRENRYMSVD DAVSEEATVV RVDSFLSQFP VEKLGLVSLF
CGFHARALFY WEQHLRMSKE KTPSSVTEND YRILQEIYSG IDDPDGIEAI SFNFHNYSLD
QQIILHENSE NWDSALTCYE LSYEKQPKND ILKVGMLNCL LQAGHYESVI NNAKHISSLV
EEPSRSTVLN LAIEAAWSAL LKDKLQELVT SSSSVSFDTK VGRLFLNYLS NMHDQAEFDQ
LIRTLYTDAT IAIANTGANS SYDCYDILSK LHAIHDFSVI SSYTLSKTPI PEDVASVFKT
RLECVVPYGK FKQRILSAQL VGFSRGSKNS VYQAPTHLEI AKLARKNQQM QRAYNSILRA
MSLNKAMAAV EHSRWWWHQG QRRKAIAELQ SSFDASLFES ADVLSMHSIS ALSSTSSNES
KGSVVKGKAL LTLTKWLGEA GQLGLKDLEV YYYKAVNIYP EWEKSHYYLA HHRVSMLEEE
KKLPPGKKSE KFLTGELVTR IINEYGRSLY YGTKHIYESM PKLLTLWLDF GAEELQLSEE
EGDSYFREHI IASRKKSLEL INSNIVRLGL KIPQYLFLTA LSQMISRICH PNQKVYRVLE
HLISLVISAY PKETLWQLMA ATKSTSHKRS VRGKSILNLL YSKKKNQPEN LEMKKLCQSA
VCLTDKLLDL CNVRLDTRAV KLSLKGHFRI SLNSPVDLVI PAQSFMNITL PSKGIVYHQY
HPFPNNQPTI EGFEDEVDIM NSLQKPRKVT IRGTNGKMYH FLCKPKDDLR KDARLMELNN
VINKILRKDQ AASRRKLNIR TYVVIPLNEE CGFIEWVNNT RPFRDILLKI YKQKNVPIPY
QELRANLDMA LQTPNPAKIF EEKILPRFPS VFHEWFMEAF PEPSSWVKSR QNYSRTLAVM
SMVGYVLGLG DRHGENILFD ELNGDAIHVD FNCLFEKGLT FAKPEKVPFR LTHNMVDAMG
PTGYEGSFRK SSEITMRLLR SNQNTLMSVL ESFLYDPLVE WNRRGQNSLS GGLEQNEATQ
VLAKIRRKLQ GYIGEEPLPL SVEGQVQELI KEAVSSDNLV QMYIGWAAYF
//
