ID B7H6C2_BACC4 Unreviewed; 565 AA.
AC B7H6C2;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN OrderedLocusNames=BCB4264_A3392 {ECO:0000313|EMBL:ACK59004.1};
OS Bacillus cereus (strain B4264).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=405532 {ECO:0000313|EMBL:ACK59004.1, ECO:0000313|Proteomes:UP000007096};
RN [1] {ECO:0000313|EMBL:ACK59004.1, ECO:0000313|Proteomes:UP000007096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B4264 {ECO:0000313|EMBL:ACK59004.1,
RC ECO:0000313|Proteomes:UP000007096};
RA Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A.,
RA Ravel J., Sutton G.;
RT "Genome sequence of Bacillus cereus B4264.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947,
CC ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613,
CC ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; CP001176; ACK59004.1; -; Genomic_DNA.
DR RefSeq; WP_001058955.1; NZ_VEHB01000007.1.
DR AlphaFoldDB; B7H6C2; -.
DR KEGG; bcb:BCB4264_A3392; -.
DR HOGENOM; CLU_008590_2_2_9; -.
DR Proteomes; UP000007096; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF3; NEUTRAL PROTEASE B; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Secreted {ECO:0000256|ARBA:ARBA00022525, ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT DOMAIN 81..129
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 245..398
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 402..563
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 392
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 481
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 565 AA; 62483 MW; C4F89259F0701B1B CRC64;
MNNFVKVGLT TGVVLSAIMP YGGVHAETED LKVETKEDTF RTGNLTAPSQ NSAENVAKDA
LKGKTEQALS SKQVNTESKV NYNVTQSRKS YDGTTLVRLQ QTYEGRDVYG YQLTAHINDD
GVLTSVSGDS AQNLQQQEDL KQPITLSEED AKKQLFNIYG NDLTFIEEPE IKQVVYVDEN
TNKATNAYQI TFSASTPEYV SGTVLIDAFG GNLLKELVQK LGIQVESSIV QSATSNKSTD
PSKLTGTGKD DLGINRTFGI TQRSDGTYML ADYSRGKGIE TYTANYKDYN NYRRNVWGYL
DDLVTSNSTN FTDPKAVSAH YLATKVYDFY QEKYGRNSFD NNGQKVISVV HGWNTNGTNK
GNPKQWFNAF SNGAMLVYGD PIVRAFDVAG HEFTHAVTRN ESGLEYAGEA GAINEALSDI
LGVAVEKYAN NGKFNWTMGE QSGRIFRDMK NPSSISSRYP EDYRHYNNLP IDADHDHGGV
HTNSSIINKV AYLIASGGSH NGVNVHGIGE DKMFDIFYYA NTDELNMTSD FKELKEACIR
VATNLYGKDS LEVQAVQQAF KAAYI
//
