ID B7HR47_BACC7 Unreviewed; 295 AA.
AC B7HR47;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=isochorismatase {ECO:0000256|ARBA:ARBA00012100};
DE EC=3.3.2.1 {ECO:0000256|ARBA:ARBA00012100};
GN Name=bacB {ECO:0000313|EMBL:ACJ78132.1};
GN OrderedLocusNames=BCAH187_A2471 {ECO:0000313|EMBL:ACJ78132.1};
OS Bacillus cereus (strain AH187).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=405534 {ECO:0000313|EMBL:ACJ78132.1, ECO:0000313|Proteomes:UP000002214};
RN [1] {ECO:0000313|EMBL:ACJ78132.1, ECO:0000313|Proteomes:UP000002214}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AH187 {ECO:0000313|EMBL:ACJ78132.1,
RC ECO:0000313|Proteomes:UP000002214};
RA Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B.,
RA Okstad O.A., Ravel J., Sutton G.;
RT "Genome sequence of Bacillus cereus AH187.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000128};
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|PIRSR:PIRSR001111-50};
CC Note=Binds 1 phosphopantetheine covalently.
CC {ECO:0000256|PIRSR:PIRSR001111-50};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- SIMILARITY: Belongs to the isochorismatase family.
CC {ECO:0000256|ARBA:ARBA00006336}.
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DR EMBL; CP001177; ACJ78132.1; -; Genomic_DNA.
DR AlphaFoldDB; B7HR47; -.
DR KEGG; bcr:BCAH187_A2471; -.
DR HOGENOM; CLU_068979_2_0_9; -.
DR Proteomes; UP000002214; Chromosome.
DR GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR CDD; cd01013; isochorismatase; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.50.850; Isochorismatase-like; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR016291; Isochorismatase.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR PANTHER; PTHR43540:SF3; ENTEROBACTIN SYNTHASE COMPONENT B; 1.
DR PANTHER; PTHR43540; PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED; 1.
DR Pfam; PF00857; Isochorismatase; 1.
DR Pfam; PF00550; PP-binding; 1.
DR PIRSF; PIRSF001111; Isochorismatase; 1.
DR PRINTS; PR01398; ISCHRISMTASE.
DR SUPFAM; SSF47336; ACP-like; 1.
DR SUPFAM; SSF52499; Isochorismatase-like hydrolases; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ACJ78132.1};
KW Phosphopantetheine {ECO:0000256|PIRSR:PIRSR001111-50};
KW Phosphoprotein {ECO:0000256|PIRSR:PIRSR001111-50}.
FT DOMAIN 215..291
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT MOD_RES 252
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000256|PIRSR:PIRSR001111-50"
SQ SEQUENCE 295 AA; 33467 MW; 93B6B73F72C6564E CRC64;
MAIPSISVYK MPIESELPKN KVDWKPDPKR AVLLIHDMQE YFLDAYSDKE SPKVELISNI
KVIREKCKEL GIPVVYTAQP GGQTLEQRGL LQDFWGDGIP AGPDKKKIVD ELTPDEDDIF
LTKWRYSAFK KTNLLEILNE QGRDQLIICG IYAHIGCLLT ACEAFMDGIQ PFFVADAVAD
FSLEHHKQAL EYASNRCAVT TSTNSLLTEL QGLKDDDEIT LQKVHELVAQ LLREPVESVG
TDEDLLNRGL DSVRIMSLVE KWRREGKEIT FADLAENPTV VDWHRLLSLQ TERVL
//
