ID B7PUM9_IXOSC Unreviewed; 702 AA.
AC B7PUM9;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=Ubiquitin-like modifier-activating enzyme ATG7 {ECO:0000256|ARBA:ARBA00017647, ECO:0000256|RuleBase:RU366022};
DE AltName: Full=Autophagy-related protein 7 {ECO:0000256|RuleBase:RU366022};
GN ORFNames=IscW_ISCW008151 {ECO:0000313|EMBL:EEC10301.1};
OS Ixodes scapularis (Black-legged tick) (Deer tick).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX NCBI_TaxID=6945;
RN [1] {ECO:0000313|EMBL:EEC10301.1, ECO:0000313|Proteomes:UP000001555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Wikel {ECO:0000313|Proteomes:UP000001555}, and Wikel colony
RC {ECO:0000313|EMBL:EEC10301.1};
RG Ixodes scapularis Genome Project Consortium;
RA Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P.,
RA Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M.,
RA Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A., Birren B.,
RA Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R.;
RT "Annotation of Ixodes scapularis.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:ISCW008151-PA}
RP IDENTIFICATION.
RC STRAIN=wikel {ECO:0000313|EnsemblMetazoa:ISCW008151-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- FUNCTION: E1-like activating enzyme involved in the 2 ubiquitin-like
CC systems required for autophagy. {ECO:0000256|RuleBase:RU366022}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|RuleBase:RU366022}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU366022}.
CC Preautophagosomal structure {ECO:0000256|RuleBase:RU366022}.
CC -!- SIMILARITY: Belongs to the ATG7 family. {ECO:0000256|ARBA:ARBA00010931,
CC ECO:0000256|RuleBase:RU366022}.
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DR EMBL; ABJB010027816; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010267751; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DS793718; EEC10301.1; -; Genomic_DNA.
DR RefSeq; XP_002406338.1; XM_002406294.1.
DR AlphaFoldDB; B7PUM9; -.
DR STRING; 6945.B7PUM9; -.
DR PaxDb; 6945-B7PUM9; -.
DR EnsemblMetazoa; ISCW008151-RA; ISCW008151-PA; ISCW008151.
DR KEGG; isc:IscW_ISCW008151; -.
DR VEuPathDB; VectorBase:ISCI008151; -.
DR VEuPathDB; VectorBase:ISCP_027870; -.
DR VEuPathDB; VectorBase:ISCW008151; -.
DR HOGENOM; CLU_012998_2_1_1; -.
DR InParanoid; B7PUM9; -.
DR OMA; RQIWDAI; -.
DR Proteomes; UP000001555; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000407; C:phagophore assembly site; IBA:GO_Central.
DR GO; GO:0019778; F:Atg12 activating enzyme activity; IBA:GO_Central.
DR GO; GO:0019779; F:Atg8 activating enzyme activity; IBA:GO_Central.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IBA:GO_Central.
DR GO; GO:0044804; P:nucleophagy; IBA:GO_Central.
DR GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IBA:GO_Central.
DR GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-UniRule.
DR CDD; cd01486; Apg7; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.40.140.100; Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain; 1.
DR Gene3D; 3.40.140.70; Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain; 1.
DR InterPro; IPR006285; Atg7.
DR InterPro; IPR032197; Atg7_N.
DR InterPro; IPR042522; Atg7_N_1.
DR InterPro; IPR042523; Atg7_N_2.
DR InterPro; IPR045886; ThiF/MoeB/HesA.
DR InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR InterPro; IPR035985; Ubiquitin-activating_enz.
DR NCBIfam; TIGR01381; E1_like_apg7; 1.
DR PANTHER; PTHR10953; UBIQUITIN-ACTIVATING ENZYME E1; 1.
DR PANTHER; PTHR10953:SF3; UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7; 1.
DR Pfam; PF16420; ATG7_N; 1.
DR Pfam; PF00899; ThiF; 1.
DR SUPFAM; SSF69572; Activating enzymes of the ubiquitin-like proteins; 1.
PE 1: Evidence at protein level;
KW Autophagy {ECO:0000256|ARBA:ARBA00023006, ECO:0000256|RuleBase:RU366022};
KW Cytoplasm {ECO:0000256|RuleBase:RU366022};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW ECO:0000256|RuleBase:RU366022};
KW Proteomics identification {ECO:0007829|PeptideAtlas:B7PUM9};
KW Reference proteome {ECO:0000313|Proteomes:UP000001555};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU366022};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366022}.
FT DOMAIN 12..326
FT /note="Ubiquitin-like modifier-activating enzyme Atg7 N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF16420"
FT DOMAIN 341..600
FT /note="THIF-type NAD/FAD binding fold"
FT /evidence="ECO:0000259|Pfam:PF00899"
FT REGION 515..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 573
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606285-1"
SQ SEQUENCE 702 AA; 76444 MW; CBCFCC9EF089B291 CRC64;
MSEATKDSAY PVQFVPFSSA LDGTFWSEIS RRKLEVVRLE EGPVPAQAWY CCDSAVGLPA
LANLDHTAFD MSSHSTGANI PGSCQLRGSI WLPNSLEAMK KLDKTALLEA AGQNIWDAIE
SESALSDPGT LNAFIGIVYV DIKSYKFWYW FAFPAVCLPD AVVFAGPPTK LPDVMKDDLV
SQLDAAYGKL KLADRAAFLV DADSSGALKI LPLSKLNEVY ESGRRFYVGY TDPSTSEQHP
GWPLRNLLGM LSRCWLGSKE RKVLPLLAYR RQGREGQLWS SRSLVFSVRF GGDALEQTFT
TPRFVGWERN AAGQLGPRMV NLSGGMDPKK VAETAVGLNL QLMRWRLAPT LNLEVIANTR
CLLLGAGTLG CNVARSLMGW GVRTITFVDA GRVSYSNPVR QSLYILSDCE NGGRHKADAA
ADALKRVFPA MDARGVVLSI PMPGHGVPSG SEDTIRKQVE QLEQLVAEHD AIFLLLDTRE
ARWLPTLMAC ARGKLVINAA LGFDTFLVMR HGVGERSEVD SDKPSRCGEG PSTSGDGQFV
HEQLSADQLG CYFCNDVVGP TDSTRDRTLD QQCTVTRPGV SMMAGALAVE LLVGLLQHPE
KGRAPAGAGS WDAPCDNPLG MVPHQIRGFI GRHHYMTLAC TAFSMCTACS PAVVSQYNAD
GWEFVAHVLR DASYLEELTG LSRLHAETDL DQVWALSDSE GD
//
