ID B7U9E0_AERME Unreviewed; 263 AA.
AC B7U9E0;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=Aminoglycoside (3'') (9) adenylyltransferase {ECO:0000256|ARBA:ARBA00035252, ECO:0000256|PIRNR:PIRNR000819};
DE EC=2.7.7.47 {ECO:0000256|ARBA:ARBA00035126, ECO:0000256|PIRNR:PIRNR000819};
GN Name=aadA1 {ECO:0000313|EMBL:ACK43799.1};
GN ORFNames=E4185_18560 {ECO:0000313|EMBL:QJT28718.1};
OS Aeromonas media.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=651 {ECO:0000313|EMBL:ACK43799.1};
RN [1] {ECO:0000313|EMBL:ACK43799.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ER.1.5 {ECO:0000313|EMBL:ACK43799.1};
RX PubMed=22127350; DOI=10.1016/j.resmic.2011.10.010;
RA Moura A., Pereira C., Henriques I., Correia A.;
RT "Novel gene cassettes and integrons in antibiotic-resistant bacteria
RT isolated from urban wastewaters.";
RL Res. Microbiol. 163:92-100(2012).
RN [2] {ECO:0000313|EMBL:AUW54057.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A-309 {ECO:0000313|EMBL:AUW54057.1};
RX PubMed=29374269;
RA Amos G.C.A., Ploumakis S., Zhang L., Hawkey P.M., Gaze W.H.,
RA Wellington E.M.H.;
RT "The widespread dissemination of integrons throughout bacterial communities
RT in a riverine system.";
RL ISME J. 0:0-0(2018).
RN [3] {ECO:0000313|EMBL:QJT28718.1, ECO:0000313|Proteomes:UP000501901}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R1-18 {ECO:0000313|EMBL:QJT28718.1,
RC ECO:0000313|Proteomes:UP000501901};
RA Shi Y., Tian Z., Zhang Y., Zhang H., Yang M.;
RT "Novel transposon Tn6433 accelerates the dissemination of tet(E) in
RT Aeromonas from aerobic biofilm under oxytetracycline stress.";
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC Evidence={ECO:0000256|ARBA:ARBA00001672,
CC ECO:0000256|PIRNR:PIRNR000819};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate;
CC Xref=Rhea:RHEA:20245, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58007, ChEBI:CHEBI:58605; EC=2.7.7.47;
CC Evidence={ECO:0000256|ARBA:ARBA00035070,
CC ECO:0000256|PIRNR:PIRNR000819};
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DR EMBL; FJ460176; ACK43799.1; -; Genomic_DNA.
DR EMBL; MF686106; AUW54057.1; -; Genomic_DNA.
DR EMBL; CP038443; QJT28718.1; -; Genomic_DNA.
DR AlphaFoldDB; B7U9E0; -.
DR SMR; B7U9E0; -.
DR Proteomes; UP000501901; Chromosome.
DR GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR GO; GO:0009012; F:aminoglycoside 3''-adenylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd05403; NT_KNTase_like; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR024172; AadA/Aad9.
DR InterPro; IPR025184; AadA_C.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR Pfam; PF13427; AadA_C; 1.
DR Pfam; PF01909; NTP_transf_2; 1.
DR PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
PE 4: Predicted;
KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW ECO:0000256|PIRNR:PIRNR000819};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotidyltransferase {ECO:0000256|PIRNR:PIRNR000819,
KW ECO:0000313|EMBL:AUW54057.1};
KW Transferase {ECO:0000256|PIRNR:PIRNR000819, ECO:0000313|EMBL:ACK43799.1}.
FT DOMAIN 28..76
FT /note="Polymerase nucleotidyl transferase"
FT /evidence="ECO:0000259|Pfam:PF01909"
FT DOMAIN 152..254
FT /note="Adenylyltransferase AadA C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13427"
SQ SEQUENCE 263 AA; 29332 MW; D2CEAC8B7D403FD2 CRC64;
MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE
TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR YPAKRELQFG EWQRNDILAG
IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA
GDERNVVLTL SRIWYSAVTG KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA
SRADQLEEFV HYVKGEITKV VGK
//
