ID B8BHF7_ORYSI Unreviewed; 974 AA.
AC B8BHF7;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 22-FEB-2023, entry version 67.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN ORFNames=OsI_34005 {ECO:0000313|EMBL:EEC67156.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946 {ECO:0000313|EMBL:EEC67156.1, ECO:0000313|Proteomes:UP000007015};
RN [1] {ECO:0000313|EMBL:EEC67156.1, ECO:0000313|Proteomes:UP000007015}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11 {ECO:0000313|Proteomes:UP000007015};
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C., Chen H., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA Zheng W., Hao B., Liu S., Wang W., Yuan L., Cao M., McDermott J.,
RA Samudrala R., Wang J., Wong G.K., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; CM000135; EEC67156.1; -; Genomic_DNA.
DR AlphaFoldDB; B8BHF7; -.
DR STRING; 39946.B8BHF7; -.
DR CAZy; GH116; Glycoside Hydrolase Family 116.
DR EnsemblPlants; BGIOSGA031703-TA; BGIOSGA031703-PA; BGIOSGA031703.
DR Gramene; BGIOSGA031703-TA; BGIOSGA031703-PA; BGIOSGA031703.
DR HOGENOM; CLU_006322_1_0_1; -.
DR OMA; TSPVMEN; -.
DR Proteomes; UP000007015; Chromosome 10.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000007015}.
FT DOMAIN 102..421
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 557..918
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
FT REGION 85..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 464..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..497
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 974 AA; 109268 MW; EC0204156BF1485F CRC64;
MVSGNLFHCR RNSWPAEEYV GRTALQLLDF DGGSPPEQAW RRRLNSHANL LKEFSVTFME
AMRMMSLGLR LWSYVREEAS HGRKAPIDPF TKEKCKPSAS QGVPLGGMGS GSISRGFRGE
FKNWHIIPGL CETSPVMENQ FSIFVSRDGG NKKYSSVLSP GHHEGLKKCN DSGISSWDWN
LSGQHSTYHA LFPRAWTVYD GEPDPDLKIS CRQISPFIPH DYKDSSLPTS VFVYTLVNTG
KDRAKVSLLM TWANSIGGFS HHSGGHFNEP FIAEDGVSGV LLHHKTAKDN PPVTFAIAAC
ETQNVNVTVL PVFGLSGEGH DSAKQMWDRM KQNGHFDREN FEAGTSMPSS SGETLCAAVS
ASTWVEPHGR CTVVFGLAWS SPKIKFQKGC TYNRRYTEFY GTSERSAVNL VHDALTKYRI
WEEEIEKWQN PILKNERLPE WYKFTLFNEL YFLVAGGTVW TDGQPPVIDE KPSPGSNQQK
SSKRGTRDTK QESVKDNHVK LTAEQVTNGG DLANGEEQSV SKYAAVHGPQ MAKATNGLGS
QEPIPYLLSK NGPENVGKFL YLEGVEYIMW CTYDVHFYAS FALLDLFPKI ELSIQRDFAN
AVLYEDRRRM KFLADGTSGI RKVKGAVPHD LGTHDPWHEM NAYNIHDTSK WKDLNPKFVL
QVYRDFAATG DMSFGRDVWP AVCAAMDYMN QFDRDGDGLI ENDGFPDQTY DAWTVHGISA
YCGGLWLAAL QAAATMAHRL GDRPFAEKYK LKFIQAKAVY EAKLWNGSYF NYDSGTSSNS
RSIQADQLAG QWYAASSGLP PLFDENKIRS ALQKIFEFNV MKVKGGRLGA VNGMTPNGKV
DETCMQSREI WTGVTYGVAA NMLLHGMEHQ GFTTAEGIFI AGWSEEGYGY WFQTPEGWTI
DGHYRSLIYM RPLAIWAMQW ARSPPKAILD APKVNLMDRI HLSPQMIRAM NEINVRKIAP
DNRCFPSSAF RCEC
//
