ID B8EPL8_METSB Unreviewed; 965 AA.
AC B8EPL8;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE SubName: Full=Diguanylate cyclase/phosphodiesterase {ECO:0000313|EMBL:ACK50223.1};
GN OrderedLocusNames=Msil_1254 {ECO:0000313|EMBL:ACK50223.1};
OS Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB
OS 13906 / BL2).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Beijerinckiaceae; Methylocella.
OX NCBI_TaxID=395965 {ECO:0000313|EMBL:ACK50223.1, ECO:0000313|Proteomes:UP000002257};
RN [1] {ECO:0000313|EMBL:ACK50223.1, ECO:0000313|Proteomes:UP000002257}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2
RC {ECO:0000313|Proteomes:UP000002257};
RX PubMed=20472789; DOI=10.1128/JB.00506-10;
RA Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT "Complete genome sequence of the aerobic facultative methanotroph
RT Methylocella silvestris BL2.";
RL J. Bacteriol. 192:3840-3841(2010).
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DR EMBL; CP001280; ACK50223.1; -; Genomic_DNA.
DR RefSeq; WP_012590293.1; NC_011666.1.
DR AlphaFoldDB; B8EPL8; -.
DR STRING; 395965.Msil_1254; -.
DR KEGG; msl:Msil_1254; -.
DR eggNOG; COG5001; Bacteria.
DR HOGENOM; CLU_000445_70_49_5; -.
DR OrthoDB; 9814202at2; -.
DR Proteomes; UP000002257; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR CDD; cd01948; EAL; 1.
DR CDD; cd01949; GGDEF; 1.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.20.20.450; EAL domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR InterPro; IPR011622; 7TMR_DISM_rcpt_extracell_dom2.
DR InterPro; IPR001633; EAL_dom.
DR InterPro; IPR035919; EAL_sf.
DR InterPro; IPR000160; GGDEF_dom.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013655; PAS_fold_3.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR NCBIfam; TIGR00254; GGDEF; 1.
DR NCBIfam; TIGR00229; sensory_box; 1.
DR PANTHER; PTHR44757:SF2; BIOFILM ARCHITECTURE MAINTENANCE PROTEIN MBAA; 1.
DR PANTHER; PTHR44757; DIGUANYLATE CYCLASE DGCP; 1.
DR Pfam; PF07696; 7TMR-DISMED2; 1.
DR Pfam; PF00563; EAL; 1.
DR Pfam; PF00990; GGDEF; 1.
DR Pfam; PF08447; PAS_3; 1.
DR SMART; SM00052; EAL; 1.
DR SMART; SM00267; GGDEF; 1.
DR SMART; SM00086; PAC; 1.
DR SUPFAM; SSF141868; EAL domain-like; 1.
DR SUPFAM; SSF55073; Nucleotide cyclase; 1.
DR SUPFAM; SSF55785; PYP-like sensor domain (PAS domain); 1.
DR PROSITE; PS50883; EAL; 1.
DR PROSITE; PS50887; GGDEF; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002257};
KW Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..31
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 32..965
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002868658"
FT TRANSMEM 189..211
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 218..240
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 252..273
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 285..304
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 310..331
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 338..360
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 418..475
FT /note="PAS"
FT /evidence="ECO:0000259|PROSITE:PS50112"
FT DOMAIN 479..531
FT /note="PAC"
FT /evidence="ECO:0000259|PROSITE:PS50113"
FT DOMAIN 564..698
FT /note="GGDEF"
FT /evidence="ECO:0000259|PROSITE:PS50887"
FT DOMAIN 707..962
FT /note="EAL"
FT /evidence="ECO:0000259|PROSITE:PS50883"
SQ SEQUENCE 965 AA; 105371 MW; EED69A82C3EA5A50 CRC64;
MAQGFAALGR CLFLLCALAL TLAGALGPAN AIESVRVPLD SQVIDLTKAV ESYNSDGDRL
LVSTAPGSDG IVRRIEVRAR EEGTRPNWIV FALTNDTSEQ IERLIVAPHF RLVGSGVIWP
DLGATRISAI TASQGFPPER EDSEDADVFR LTLDPGTTIT YVAELRTPNL PQLHLWEPDA
YKDRMTSLAL YKGIIIGVAG LLALFLTIVF VVRGAVIFPA AAALAWTVLA YVCIDFGFWG
RIIGSEGQSD RIWRAGTESI LAATLLVFLF AYLNLNRWHV RASHVAAIWL IFLAALVGLS
VYDAPVAAGV ARISIATIGA VGFVLVLYLA IHGYDRAVML IPTWFLLLAW IVGAAFTVTG
ALTNDLVSPA LIGGLVLIVM LIGFTIMQNA FVGAGLAHGA VPDAERKALA FTGSGDIIFD
WDVSADRVYV SPEVEAQLGL QRGALEGPAS NWFDVLHQSE RDRYRACLDA MLEQRRGRIN
QDFRLRAADG QHFWFRLKAR PVIGADGEVV RIVGVLSDIT EIKTAEERLL HDAVHDNLTG
LPNRELFFDR LDAALSLAQS DPKIRPAVMS IDIDRFKQIN EQVGLSAGDS ILLTVARRLG
RILKPQDTLA RLSGDQFILL IVSESEADQI TALANTARRV ASTPVSFSDK EIPLTASIGL
AFYDPQLHGK REDMLKDAEI AMRHGKRLGG NRIEVFRATM RSLRSDRFGL AADLKRALDR
GEMKVVFQPI VRLEDRTVAG FEALLRWDHP RLGRLNPGEF ISVAEETGVI IDLGIFAMDR
AARELAVWQR ALDVDPPIFV SVNVSSRQLL RHDLLQDVKS VLSRVEIAPG SLKLELTESL
VMENPEYAAQ ILTRIRDLGA GLSLDDFGTG YSSLAYLQRF PFDTIKIDRS FVRQNGNGAR
PVILRSIVAL AHDLGMEVVA EGAEAESDAI ELYQLGCEFA QGFAFGHPIS AAEARKLVGA
ATIAA
//
