ID B8NXY5_ASPFN Unreviewed; 515 AA.
AC B8NXY5;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 24-JAN-2024, entry version 59.
DE SubName: Full=Xylosidase/arabinosidase, putative {ECO:0000313|EMBL:EED45071.1};
GN ORFNames=AFLA_009550 {ECO:0000313|EMBL:EED45071.1}, G4B84_011439
GN {ECO:0000313|EMBL:QMW35910.1};
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952 {ECO:0000313|EMBL:EED45071.1, ECO:0000313|Proteomes:UP000001875};
RN [1] {ECO:0000313|EMBL:EED45071.1, ECO:0000313|Proteomes:UP000001875}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 /
RC SRRC 167 {ECO:0000313|Proteomes:UP000001875}, and NRRL3357
RC {ECO:0000313|EMBL:EED45071.1};
RX PubMed=25883274; DOI=10.1128/genomeA.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
RN [2] {ECO:0000313|EMBL:QMW35910.1, ECO:0000313|Proteomes:UP000515286}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL3357 {ECO:0000313|EMBL:QMW35910.1,
RC ECO:0000313|Proteomes:UP000515286};
RA Fountain J.C., Clevenger J.P., Nadon B., Youngblood R.C., Korani W.,
RA Chang P.-K., Starr D., Wang H., Isett B., Johnston H.R., Wiggins R.,
RA Chu Y., Agarwal G., Kemerait R.C., Pandey M.K., Bhatnagar D.,
RA Ozias-Akins P., Varshney R.K., Scheffler B.E., Vaughn J.N., Guo B.;
RT "Two New Chromosome-Level Aspergillus flavus Reference Genomes Reveal a
RT Large Insertion Potentially Contributing to Isolate Stress Tolerance and
RT Aflatoxin Production.";
RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR EMBL; EQ963486; EED45071.1; -; Genomic_DNA.
DR EMBL; CP059873; QMW35910.1; -; Genomic_DNA.
DR RefSeq; XP_002385200.1; XM_002385159.1.
DR AlphaFoldDB; B8NXY5; -.
DR EnsemblFungi; EED45071; EED45071; AFLA_009550.
DR VEuPathDB; FungiDB:AFLA_013257; -.
DR eggNOG; ENOG502SHCU; Eukaryota.
DR HOGENOM; CLU_016508_2_0_1; -.
DR OMA; GTHEGHM; -.
DR OrthoDB; 1891044at2759; -.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR Proteomes; UP000515286; Chromosome 8.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187}.
FT DOMAIN 328..513
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 197
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 143
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 515 AA; 57348 MW; DE874EDE6A47F278 CRC64;
MSDTVNPIIP GFAPDPSVVL VDGWFYLVNS SFHAFPGLPI YASQDLVSWK QIGNAIHRQS
QLSLAKSQAN LYPVDDGKFL VGSGGLYAPT IRHHQGTFYV VCTNVIRAEG KNRDVTENFI
VSTNDIWSGT WSNPVYFGFE GIDPSLLFDD DQKVYMQGSG GIGPGTTINL FEINLKTGAR
LSEEKIIWRG TGDIYPEGPH LYKHKGWYYL LIAEGGTHGG HMVTMARSQN VFGPYDSCPN
NPVLTARDTQ EYIQYTGHCE LFKDEKAQWW GVCLGGRLDT QGRCPMGRET FLTRVDWDGE
WPVFDQVELN PRGLLATRSS PRLTAEGGLD YLYIRDAIMS NYRLENDHSS LTLTASSVDL
SHPADSPTFI GKRQRKLAGT STVVLKGIED SWSSAKIKAG LACYKEEHRF SRIYYDADQQ
AVVLELVNAA KKIVRTEKHT LGEAPCSLLF RIEYTEKQYS LLYSFEPHVG NDWTCLGTVD
TLDITDPDFT GPIIGVYAVG QMEGVQVQFD GLSVE
//
