ID C0EFI6_9FIRM Unreviewed; 784 AA.
AC C0EFI6;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=CLOSTMETH_02628 {ECO:0000313|EMBL:EEG29795.1};
OS [Clostridium] methylpentosum DSM 5476.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Oscillospiraceae incertae sedis.
OX NCBI_TaxID=537013 {ECO:0000313|EMBL:EEG29795.1, ECO:0000313|Proteomes:UP000003340};
RN [1] {ECO:0000313|EMBL:EEG29795.1, ECO:0000313|Proteomes:UP000003340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5476 {ECO:0000313|EMBL:EEG29795.1,
RC ECO:0000313|Proteomes:UP000003340};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEG29795.1, ECO:0000313|Proteomes:UP000003340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5476 {ECO:0000313|EMBL:EEG29795.1,
RC ECO:0000313|Proteomes:UP000003340};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Clostridium methylpentosum (DSM 5476).";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEG29795.1}.
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DR EMBL; ACEC01000091; EEG29795.1; -; Genomic_DNA.
DR AlphaFoldDB; C0EFI6; -.
DR STRING; 537013.CLOSTMETH_02628; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_9; -.
DR Proteomes; UP000003340; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 2.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000003340}.
FT DOMAIN 20..341
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 342..614
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 41..48
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 784 AA; 88453 MW; 364110F6FC2F2D9E CRC64;
MSIEQQFHQL KKQILQNKFS RMNEMQRKAV FTVNGPLLIL AGAGSGKTTV IVNRIAYLVN
YGNAYFSDQL PDGLCAEDIV FLENMASGKF EDEGMFRRLV ADHAPRPWNV LAITFTNKAA
GELKERLEAM LGDAALDINA GTFHSQCMRI LRRNIDRLGY SSSFTVYDTD DSVRVIKDSL
SALGLSDKVY PPKAVLGEIS RAKDQMLTPE EYERQAGSDF RRIELAKIYR SYQHQLKSSN
ALDFDDIICL TVVLLEQNPD LLERYRDRFR YILVDEYQDT NHAQYRLVSL LSGGHQNLCV
VGDDDQSIYK FRGATIENIL SFEKQFQNAT VIRLEQNYRS TQNILSAANA VIENNTGRKG
KNLWTDKGDG EPVSVCRVNN ENAEADFIAS TISQDIESGM KFRDHAILYR MNAQSATIER
YFVRAGIPYR IVGGTKFYDR KEIKDILAYM SVVNNTGDNL RLKRIINEPK RGIGPGTVGK
VQEIADGLGL AMFDVLRNAD TYAALVKKAA SLRDFSNMIE QLREDAVELP LDEFLDSLIA
RTGYVDELKK QGKEGQTRIE NIQELKTNLI KFRQENDDAT LGGFLEEVAL YTDLDSFNAS
DDSVTMMTMH SAKGLEFPVV FIAGMEEGIF PGVRSIGEPD ELEEERRLAY VGITRAKKKL
YITNAAQRML FGNTKYARPS RFIGEIPEHL VDSEDKTVSR VINFEEESRR VVQPQINVDG
VGLRHKAEAI DIDYTTGDRV RHSVFGEGTI LSMQPMGNDV LVEVAFDKKG NKKIMANFAK
LKKL
//
