ID C3ZIQ3_BRAFL Unreviewed; 3516 AA.
AC C3ZIQ3;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012494};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
GN ORFNames=BRAFLDRAFT_105111 {ECO:0000313|EMBL:EEN47588.1};
OS Branchiostoma floridae (Florida lancelet) (Amphioxus).
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomatidae; Branchiostoma.
OX NCBI_TaxID=7739;
RN [1] {ECO:0000313|EMBL:EEN47588.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 {ECO:0000313|EMBL:EEN47588.1};
RC TISSUE=Testes {ECO:0000313|EMBL:EEN47588.1};
RX PubMed=18563158; DOI=10.1038/nature06967;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Putnam N.H., Butts T., Ferrier D.E.K., Furlong R.F., Hellsten U.,
RA Kawashima T., Robinson-Rechavi M., Shoguchi E., Terry A., Yu J.-K.,
RA Benito-Gutierrez E.L., Dubchak I., Garcia-Fernandez J., Gibson-Brown J.J.,
RA Grigoriev I.V., Horton A.C., de Jong P.J., Jurka J., Kapitonov V.V.,
RA Kohara Y., Kuroki Y., Lindquist E., Lucas S., Osoegawa K., Pennacchio L.A.,
RA Salamov A.A., Satou Y., Sauka-Spengler T., Schmutz J., Shin-I T.,
RA Toyoda A., Bronner-Fraser M., Fujiyama A., Holland L.Z., Holland P.W.H.,
RA Satoh N., Rokhsar D.S.;
RT "The amphioxus genome and the evolution of the chordate karyotype.";
RL Nature 453:1064-1071(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|ARBA:ARBA00024517};
CC -!- SIMILARITY: Belongs to the RdRP family.
CC {ECO:0000256|ARBA:ARBA00005762}.
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DR EMBL; GG666628; EEN47588.1; -; Genomic_DNA.
DR RefSeq; XP_002591577.1; XM_002591531.1.
DR SMR; C3ZIQ3; -.
DR STRING; 7739.C3ZIQ3; -.
DR eggNOG; KOG0988; Eukaryota.
DR eggNOG; KOG1802; Eukaryota.
DR InParanoid; C3ZIQ3; -.
DR GO; GO:0004386; F:helicase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0035082; P:axoneme assembly; IEA:InterPro.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IEA:InterPro.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProt.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR039586; CFAP46.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007855; RNA-dep_RNA_pol_euk-typ.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR15977; CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 46; 1.
DR PANTHER; PTHR15977:SF15; CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 46; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF05183; RdRP; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48452; TPR-like; 1.
PE 3: Inferred from homology;
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022484};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1341..1442
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1455..1525
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT REGION 1259..1305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1697..1731
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2061..2085
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2240..2282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2536..2571
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2685..2745
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2821..2854
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3411..3431
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1263..1279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1711..1726
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2068..2082
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2253..2275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2536..2554
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2557..2571
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2722..2738
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2830..2852
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3516 AA; 394564 MW; 0728659195123DED CRC64;
MASTDSRTLT GFLENPDTEE GVRVEQKELV YKQKRSMPQE RHQKVSIKVV YIRRKKDRED
VRGKKLTTDD FFVETDYSDA KMCSLFREHD VGHFVVLHFD LSTPPHVVSA VLRSGFKVTV
GKGQRRHYVF FGHSASQLRE RTCILYDQGV LGPWERLVNG FGEFEAIKNP SKRAARIGLL
LSTATKCLEL DEKEIDHTSD IERDGFTFTD GCGFISVACA RKIMQDKDIS ERYRHQDPPV
PSVIQFRMKG CKGVVMTDPS LPNGVCIRPS QEKFKWKLPP PHVFGVCDDG ISQPYAFGQL
NKQYIMMLSA LGIKDEVFLS KQETYFKELD CLETDKEVAF RHLCARGYIG LAERLFSREG
GPDNETLEIL RGFRSRVRRP PKANKLHKLL NTKPEPARAL KIPIEKSRNV FGVCDPSGTL
EPGQCFFQAT VRGKMQVFAD TKVAVVKCPC YHPGDFRVLQ CVNVEDCRHL VDCVVFPVQG
ERPHADEIAG SDLDGDKFFV CWDPDLVPEC EEAPLSYPGG EAAKKERVSI GDLISVFANY
NSRTVSRLNE LFNRWADVKG VTSSECRKIA KLFSTAVDTA KTGKGVDIPP RLLKPPDAQE
KRYVWQKMLE RANLYLADSV AADSDLTVRV FEEADITDLL NNKDINVSEY QLFRFLYKWC
ESNGRLQDLQ RYVGQIDFTR FTPSQKVRVA VDCPELPMEY VSNAFYRSKI LTENDIERMD
IMSPNQWSLF YRESDEDISW PTVRSVLTSE TPKLLIFEFC LAGTVLVMGL CLPGPLVMED
VITTDQSSGR PTVVFFSDHS GGGMEMVDLQ DGHRNDDYLL ELDSEHLQIY NVRRPRTFVW
LRWSDEAVRM SVALDRFPGE RRDRSVKLQK EEVQSVEVFY QVTRMQCGPS IYTERRQREG
LSEEPPQAAP EGTDVAGTAF NLNIGSPREF PELGVELDED ISEEAERLRK FESMCEAICE
DGEAGTLSSD SLNQIIHRFG QLPEAALRLE SLMWKYRDQV NHRPEAEERY FHLLINIVFM
TSYMCVQPAA STDTESTLMR IRRFCKTLQR DGVVFGLDRL RQLFIHLQYA GVHCANSLLD
MIRDGVLPIN PADFVPGHVD YYLHWSALCT MEVREEVCSS RSRALRARED EISVIRVEED
DGRYFIADWE KNELPLSVGD QLQITALPMS QASPEVIVTE VKIDRTEVEL KVLWNNGEAQ
HQCITPGQFA AVHIGNCISH RRVMAALDCL CVKADSDTPS NLMCERVISS FGGNVDGLAA
TFEQGGDEDR MRDDGIQSDA EAPPKAPGTT DATSTGPTND EDLHTDAPLE HQRTEAAFGG
SDFDAATGMR PKKRWFTKSM MNSSQEKAVQ AASSSEITLI QGPPGTGKTK AAAEVIIRWQ
GQSDNKILAV AETNEGVDNI IQKLLDRNVP QEQILRVGQP EKVRYGLRHL TLDKRYDDKY
GSGESRGGRK KPQGIKAILS KANIICTTCI GAGSQVLQQM TFRCVLVDEA AQATEPAVLC
ALSHGCQQVC LIGDDQQLPP LVQSEDAQEL KISLFERYHF LVYNASVLYW QMCRPFLKPN
HRQHLAKSLH QVVRALDEID DKDYEWRAQL MIALIECLMD SNKPKDAENI SNLAATFTRQ
NVPSLFKDVF RLQIKHKLGE PGKALRDLLK VTASGDLAVY YKICKLKTGL ESGEPHPNLH
RELFKILRQI LQQEDDMQSH ASSYSGGRKS QTLSDKEREK EISANPPSVE ERPPLLLELG
RLCLDHSFPD LATQCVDGIM ACPVKDEATM LQTEFMNCDL MVKNLKNKME SYTKSAVEVR
VLAIKRLMQA LQNAVRYGDP NVLQAGCVTQ WNMCLPLLQL NLRQHVRKPL TMVAEALEDI
DSLLILLRCQ VHTELAKCEE DQEQIEVAMD HLRKAILLDD SGQYRERLET MLHRLQLRIQ
LYKTPERQED QAAMIIEQAR KSSESGTVRM KRSLLVRAGQ ALAPDAFLLV LDSESETKAA
GGGKGVETVI TQLAAKARQF EKSVKKAAGH LKRLGDENDR ERAQLWADLA KTARKQEVWD
VCRVACRFCL LYDDGRWKPS PGEVTINESP SKEEKKGPDD APEGPVVVES TTVLYDRDLV
RMLAEVHFIN SEGVQLNNQP IPPVDKSKHP KGYVPKKPEE DPDWLYYCDW IKSLSAAATS
GFLRAAELGV ELQESWVVCS SAAYLWNYNN HLLTENRHRE LVEPFASVVE ALKKVGHAGE
AVLLTQVCNA LAYGLIQPWI PPPPPKEPTP PTRLTTDKAS SPSKKASQKS AKTPAKTPSK
AVAVDQEGMT DIKKALEVCD FALDVTSGSI ADDVVPISIR HMLIITWVRV KQMMSQQIKN
LGVEDEGNDG QRPMTRCLVA LEMTSLNGNG LMDFNVIKLD ELSGMVDECV WTEGLVELQV
WTRLAEHAHG VHNHQLVMKC AGKALEFDGK RKMEKKLGSH GTMVESEMLS YSSDILGMSL
VENMAGNNAI RRSALEAFLN AARYAGKAGN YTLVIIAARH YWNTSLKLIP SPMERELLRD
PLLVILKALA DTYREKAKPD KEEKVEEKEP DESKTAPAPT PVSTTPSFGD PSEDLTLRAS
MYGVVFQSYA DRGQWEEGLK DESEAYVALM WHRVAKNSKE PMEQLAAYQQ AIVALQTPSS
EWQKIDYLIE FAEWLFTNGF PKQDAQDMLD WAADILLNMQ FGPQFKPEET AGRSSAKRRG
KGKGKQSPPA TAKPKPPPQP QPPAKKKEED EEDGPDRYIP VSRPSQIGVE VTNSSLLFAD
LWQTRQLEAL LRIHVMLAKV AGKTSPNFKD FAVMAYRYCM RIWEASLQSV PHVIKEMARL
ATTSQSERPA SRGKGDKKET PAKTDKPKKK GPVEAIPATL EDWASYDVPE EVREAFKHDN
TGAGVNKTTI TKPTLTLHYM ETLADQLREL GYNHLALPIL TLAEVIGADV LGSKAMSSLW
HTRLLETCLE MNLLPAAGFH EQQAGPAIAE EDQAVSREEI AKWHEQQEQV RREELRLQQS
QEALGKFVPK TTTSKEDSQE TAPPWTVSGV SLREVWTDKA EVLLRLGYYQ GARTLLGEAH
KAAKTFGDQC TEVRTLKNLA RLSILEANLG QAVTLLQEAQ ALGGDEEFWF QTVLDMADAV
MREVDSDTKA KKAKSILLHA ITVYKEVLES RSTKADQFGY FVASFEAKLA TIQLEEALDT
DTNAMQDEPV DMKEVLRLFQ GCAERLLTAG YPRRAAEVMQ QHAGALRQLA RAATIPEQLH
SCLLEGRRIL GHAVSLMEEL LQDVQTLAPL HETQNMSLPL QREVAGLKVK YAELLGEIFE
AVAREDRNRT IIEARKGSLQ RMVEDYIRST PDPHGVEKEW GAVIATLPQS AIVQLTAAHS
LARGYTPLRA KSLYLTGRVL SVLSAHLNPD HPSQQWELQY LDLNAVLPDS TGRIPEEEQG
EGEVGTEPPM SDRQQAKYTQ QARQLKAGWQ QHQSVLAQAS EVLVQCLKLS LQHKYTDIAG
AAALELLECL GQYDPASASQ YLALFQVQSS LTVIGS
//
