ID C4KDM7_THASP Unreviewed; 530 AA.
AC C4KDM7; A0A5C7S7F3;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Tmz1t_3600 {ECO:0000313|EMBL:ACR02193.1};
GN ORFNames=E6Q80_19720 {ECO:0000313|EMBL:TXH79794.1};
OS Thauera aminoaromatica.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Thauera.
OX NCBI_TaxID=164330 {ECO:0000313|EMBL:ACR02193.1, ECO:0000313|Proteomes:UP000002186};
RN [1] {ECO:0000313|Proteomes:UP000002186}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MZ1T {ECO:0000313|Proteomes:UP000002186};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sayler G.S.;
RT "Complete sequence of chromosome of Thauera sp. MZ1T.";
RL Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACR02193.1, ECO:0000313|Proteomes:UP000002186}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MZ1T {ECO:0000313|EMBL:ACR02193.1,
RC ECO:0000313|Proteomes:UP000002186};
RX PubMed=23407619; DOI=10.4056/sigs.2696029;
RA Jiang K., Sanseverino J., Chauhan A., Lucas S., Copeland A., Lapidus A.,
RA Del Rio T.G., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D.,
RA Brettin T., Detter J.C., Han C., Chang Y.J., Larimer F., Land M.,
RA Hauser L., Kyrpides N.C., Mikhailova N., Moser S., Jegier P., Close D.,
RA Debruyn J.M., Wang Y., Layton A.C., Allen M.S., Sayler G.S.;
RT "Complete genome sequence of Thauera aminoaromatica strain MZ1T.";
RL Stand. Genomic Sci. 6:325-335(2012).
RN [3] {ECO:0000313|EMBL:TXH79794.1, ECO:0000313|Proteomes:UP000321192}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bin_27_1 {ECO:0000313|EMBL:TXH79794.1};
RA Stamps B.W., Spear J.R.;
RT "Metagenome Assembled Genomes from an Advanced Water Purification
RT Facility.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; CP001281; ACR02193.1; -; Genomic_DNA.
DR EMBL; SSFD01000333; TXH79794.1; -; Genomic_DNA.
DR RefSeq; WP_004299236.1; NZ_SSFD01000333.1.
DR AlphaFoldDB; C4KDM7; -.
DR STRING; 85643.Tmz1t_3600; -.
DR REBASE; 20882; M.TspMZORF3600P.
DR KEGG; tmz:Tmz1t_3600; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_018284_2_0_4; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000002186; Chromosome.
DR Proteomes; UP000321192; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF4; TYPE I RESTRICTION ENZYME ECOKI METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ACR02193.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002186};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ACR02193.1}.
FT DOMAIN 11..128
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 138..402
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 530 AA; 58857 MW; D6EA711D4DFAAF55 CRC64;
MSNPTAALDI GAKLWSLCHV LRDDGVTYHQ YLSELTYLLF LKMMKETGQE ERLRIWKAKD
KKQGSPKEEQ AGTRWDDLLG ASAPDRLDLY KEMLLDYGLH GRGAVQEIYA NANTFITKPA
TLSKLVTDID RLDWYSVDRD DLGDLYEDLL ERNAGEKKSG AGQYFTPRHL IDSIVSVMKP
QLGDVIQDPA AGTCGFLIAA NNYLRQHNDF DSLSDEAQRK YRHQTFHGME LVQDTHRLAL
MNMLLHGIEG GVTYGDTLSD DHKGLPPATL ILSNPPFGTK KGGGLPTRGD LTFETSNKQF
AFLQHIYRAL KPGGRAAVVL PDNVLFESNI GADIRRDLMD KCNLHTILRL PTGIFYAQGV
KTNVLFFTRG DTDKGNTKEV WVYDMRANMP AFGKRTPFTR DYFRTPPDVP ASQPRDKFED
VFGSDPRGGP AALAARQDTG EAGRWRRFTR EQIAQRGDSL DISWLKDSGA ASTDQSRDDP
SLLAGLALKE LNAAVSELRS LLEELGEDPD AVLEAVVADD SAEDGEGAIA
//
