ID C5H807_DANRE Unreviewed; 2248 AA.
AC C5H807;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 99.
DE RecName: Full=Phosphoinositide phospholipase C {ECO:0000256|RuleBase:RU361133};
DE EC=3.1.4.11 {ECO:0000256|RuleBase:RU361133};
GN Name=plce1 {ECO:0000313|EMBL:ACL12271.1,
GN ECO:0000313|RefSeq:NP_001155125.1,
GN ECO:0000313|ZFIN:ZDB-GENE-061212-4};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:ACL12271.1};
RN [1] {ECO:0000313|RefSeq:NP_001155125.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.D., Sun X.J., Deng M., Zhang G.W., Zhou Y., Wu X.Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.L., Fan H.Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [2] {ECO:0000313|RefSeq:NP_001155125.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=17086182; DOI=10.1038/ng1918;
RA Hinkes B., Wiggins R.C., Gbadegesin R., Vlangos C.N., Seelow D.,
RA Nurnberg G., Garg P., Verma R., Chaib H., Hoskins B.E., Ashraf S.,
RA Becker C., Hennies H.C., Goyal M., Wharram B.L., Schachter A.D.,
RA Mudumana S., Drummond I., Kerjaschki D., Waldherr R., Dietrich A.,
RA Ozaltin F., Bakkaloglu A., Cleper R., Basel-Vanagaite L., Pohl M.,
RA Griebel M., Tsygin A.N., Soylu A., Muller D., Sorli C.S., Bunney T.D.,
RA Katan M., Liu J., Attanasio M., O'toole J.F., Hasselbacher K., Mucha B.,
RA Otto E.A., Airik R., Kispert A., Kelley G.G., Smrcka A.V., Gudermann T.,
RA Holzman L.B., Nurnberg P., Hildebrandt F.;
RT "Positional cloning uncovers mutations in PLCE1 responsible for a nephrotic
RT syndrome variant that may be reversible.";
RL Nat. Genet. 38:1397-1405(2006).
RN [3] {ECO:0000313|EMBL:ACL12271.1, ECO:0000313|RefSeq:NP_001155125.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=19332147;
RA Zhou W., Hildebrandt F.;
RT "Molecular cloning and expression of phospholipase C epsilon 1 in
RT zebrafish.";
RL Gene Expr. Patterns 9:282-288(2009).
RN [4] {ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [5] {ECO:0000313|RefSeq:NP_001155125.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26469318;
RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA Reith W., Hertzano R.;
RT "RFX transcription factors are essential for hearing in mice.";
RL Nat. Commun. 6:8549-8549(2015).
RN [6] {ECO:0000313|RefSeq:NP_001155125.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [7] {ECO:0000313|RefSeq:NP_001155125.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-
CC bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-
CC diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:33179, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:58456,
CC ChEBI:CHEBI:203600; EC=3.1.4.11;
CC Evidence={ECO:0000256|RuleBase:RU361133};
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DR EMBL; EU919685; ACL12271.1; -; mRNA.
DR RefSeq; NP_001155125.1; NM_001161653.1.
DR GeneID; 568288; -.
DR KEGG; dre:568288; -.
DR AGR; ZFIN:ZDB-GENE-061212-4; -.
DR CTD; 51196; -.
DR ZFIN; ZDB-GENE-061212-4; plce1.
DR OrthoDB; 2900494at2759; -.
DR PhylomeDB; C5H807; -.
DR Proteomes; UP000000437; Chromosome 12.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; IBA:GO_Central.
DR GO; GO:0032836; P:glomerular basement membrane development; IMP:ZFIN.
DR GO; GO:0032835; P:glomerulus development; ISS:ZFIN.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR GO; GO:0051209; P:release of sequestered calcium ion into cytosol; IBA:GO_Central.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00275; C2_PLC_like; 1.
DR CDD; cd16203; EFh_PI-PLCepsilon; 1.
DR CDD; cd08596; PI-PLCc_epsilon; 1.
DR CDD; cd01780; RA2_PLC-epsilon; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR001192; PI-PLC_fam.
DR InterPro; IPR046973; PLC-epsilon1_cat.
DR InterPro; IPR028398; PLC-epsilon1_RA2.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR InterPro; IPR015359; PLC_EF-hand-like.
DR InterPro; IPR046974; PLC_epsilon1_EF.
DR InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR InterPro; IPR001711; PLipase_C_Pinositol-sp_Y.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10336:SF6; 1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE EPSILON-1; 1.
DR PANTHER; PTHR10336; PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF09279; EF-hand_like; 1.
DR Pfam; PF00388; PI-PLC-X; 1.
DR Pfam; PF00387; PI-PLC-Y; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR PRINTS; PR00390; PHPHLIPASEC.
DR SMART; SM00239; C2; 1.
DR SMART; SM00148; PLCXc; 1.
DR SMART; SM00149; PLCYc; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
DR PROSITE; PS50008; PIPLC_Y_DOMAIN; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
PE 2: Evidence at transcript level;
KW Guanine-nucleotide releasing factor {ECO:0000256|PROSITE-ProRule:PRU00168};
KW Hydrolase {ECO:0000256|RuleBase:RU361133};
KW Lipid degradation {ECO:0000256|RuleBase:RU361133};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361133};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Transducer {ECO:0000256|ARBA:ARBA00023224}.
FT DOMAIN 480..735
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT DOMAIN 1718..1808
FT /note="PI-PLC Y-box"
FT /evidence="ECO:0000259|PROSITE:PS50008"
FT DOMAIN 1814..1939
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 2090..2193
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT REGION 257..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1015..1160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1524..1596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1646..1681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2216..2248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1016..1041
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1047..1085
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1091..1157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1528..1545
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1546..1565
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1566..1596
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2248 AA; 250626 MW; 8E836032359DD414 CRC64;
MRLHVERQAH IGIKSSMASN RASPVLGVKS LGKETSSNGD ARLLMNYSLP GGTPPQTPVH
LEGDSVGTVC RIWKSLDIRS KSLDCLDETF RSKNCSCEAI RHREEGSYNF HMCGFCDSVE
NAEGCPKNTF SPRSWGTVPQ KRKAEAFSSG ITSLDEPRPT LSGSDGELCP KELVISQNME
NLPFCGLDLR RSSVPVSTPQ GPQCANGCGT AENNKMRNRL DFVCAICHKN GFSSLERLNR
NTKPMCSKGK PLKRSKPFRF SVPDSSDGEG TVSDSEFVRN RKDRSTVLVR RYLKNNQKFK
KTVCTGTKAI VKTLPTGCIS DRIWESVCNE RSRHEASGHS KALQAIQLQV SRALLAYPGA
RLAEIDHAAP TLSPEVSLLH RSRKLLAQAV SWPPVSPSQI RFRACHRRIS IAAHLSGALL
VATAALGART SLPSFTVGST GRAMLKERQL CSSMANSSIL PSAVTGISKE LADLRHLIQF
PEEIATILTE QEQQLYRRVF PLDYLSFLTR DLGSPECHKR HPHLKASLSA PIMPTQNDNH
NTVEDLVTRF NEVSSWVTWL ILTAGSMEEK REVFSYLVHV AKCSWNMGNY NAVMEFLAGL
RSRKVLKMWQ FMDQADIETM RGLKDAMAQH ESSSEYKKVV SRALNIPGCK VVPFCGVFLK
ELSEALDGAA SIIGLRPSFD SQEDPVEFVT DYNGQQHFLQ RLGSDGLHSS DKEATVSNIL
QTIRSCNRSL EAEEPEERAR EITVCPKNSF KDKSRNQKGP GTERVLFSIG DLSDSEGDPL
ETVKDVVDLQ TTEDVRGPFS HGTELIPWYV LSLQPDIHQF LLQGATVIHY DPESHLTARC
LLRLQPDNCF LTWCKPHSSC SLYGRARTFM GHPASPDHLH IGQPVHCGLS DGLLDLNVVK
AVFMGHPGVD VNYVCLQHKL CNMNPGGNGA TLLYGLHTTD NRLLHFVAPK HTARMLHEGL
QELLNSIRKI RKFPDQRLQW LRKQYVSLYQ EDGRFEGPTL AHAIELFGGR RWNMGTSGPG
SASRSAEKNS AQKNSPLGIN SNVKKKKKAL VRGDSGDGTD DEMTARKTRG CKETLGRRES
DPLENVEQGE AEDCSTFSQP GPSSLSNNKG QSPGSSSSSS TSSSVASFCT NSSTPTRPHS
SPILPTNLKS QPGAWSSRSW HGRGKGCFRG FQDLMISDSI MSFVEFVELF KSFSIRSRKD
LKELFDTYAV PCSRSGPESV PLYTTLRIDD KLTGLQPDLD LLTRNGSDLG LFIRTRQQMS
ENQKQISDAI AAASIVTNGT GVENSSLGVL GLAISQLNDF LVNCQREHLS YDEILSIIQK
FEPSSNMRQM GWMSFEGFAR FLMDKDNFAS KNEESQVNLD ELQHPLSYYY IESSHNTYLT
GHQLKGESSV ELYSQVLLQG CRSVELDCWD GDDGMPVIYH GHTLTTKIPF KDVVEAINRA
AFVNSEMPVI LSIENHCSLP QQRKMAEIFK MVFGEKLVTK FLFESDFADE PLLPSPLQLR
GKILLKNKKL KAHQAPVDIL KQKAHQLAHM QAQANNGTVS TTPLGNNDEE EEEEDEYDYD
YESLSDDNIL DDKPEGKSST EKLQYESNDE MPKRFKKAGS KGKMFDMELG EEFYLPQNEK
ESRQIAQELS DLVIYCQAIK FPGLSTLSPS GSGRGKDRKS RKSIFGSAPA RGSPGEPVTL
VRAPGKASLE GIRMNSEEQM CLSPSTSLSS IIRTPKCYHI SSVNENAAKR LCRRYSQKLI
QHTSCQLLRT YPAATRIDSA NPNPLIFWLH GVQLVALNYQ TDDLPMQLNA ALFEANGHCG
FVLKPPVLWD RSCPLYQHFY PLDRDLENMT PTLYTLTIVS GQNVCPGNSN GSPCVEVEVL
GMPADSCHFR TKPIHRNTLN PMWNEHFQFH VHFEDLAFLR IAVVENNSSQ VTAQRILPLK
TLRAGYRHLQ LRNLHNEPLE VSSLFMFSRR TEESPTGGPP SASVLFSTEE RRSVQQHKVT
VHGVPGPEPF TVICVDEFTT AKQLLDSLFP TASFKYMLME ERVSLSKEKK APQQRPLAND
ERLLKLIHSW QPEDGYVGRI YLKTREQQNC SEKTSVPLES LEELGSSEDD TFFVQVHDVS
PEQPHTVIKA PRYSTAQDII QQTLSKAKYS LSILSNPNPC DYVLMEELCK DAGGKKSSSA
KPCQRVLQDH ECVFQAQSRW KGAGKFILKL KEQLAREDKR KGVSFASELR KLTGRSRSVT
VNTNSNDTHS KEEKSACPSM SCVPETSQ
//
