ID C5NZE6_COCP7 Unreviewed; 1403 AA.
AC C5NZE6;
DT 01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 01-SEP-2009, sequence version 1.
DT 24-JAN-2024, entry version 64.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=CPC735_011570 {ECO:0000313|EMBL:EER29839.1};
OS Coccidioides posadasii (strain C735) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX NCBI_TaxID=222929 {ECO:0000313|EMBL:EER29839.1, ECO:0000313|Proteomes:UP000009084};
RN [1] {ECO:0000313|EMBL:EER29839.1, ECO:0000313|Proteomes:UP000009084}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C735 {ECO:0000313|Proteomes:UP000009084};
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EER29839.1}.
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DR EMBL; ACFW01000001; EER29839.1; -; Genomic_DNA.
DR RefSeq; XP_003071984.1; XM_003071938.1.
DR GeneID; 9697479; -.
DR KEGG; cpw:CPC735_011570; -.
DR VEuPathDB; FungiDB:CPC735_011570; -.
DR HOGENOM; CLU_001517_2_0_1; -.
DR OrthoDB; 1427975at2759; -.
DR Proteomes; UP000009084; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Transferase {ECO:0000313|EMBL:EER29839.1}.
FT DOMAIN 1..442
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 597..840
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 923..1384
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1403 AA; 159123 MW; 0F13BA2F4D8736C8 CRC64;
MPVESLSIFS IVSKFMGKFP ADWNKHLQGI GQRGYNVVHF TPLMTRGASN SPYSIYNQLE
FDRQCFPNGE SDVIDMVTRM EKEYGLLALT DVVWNHTAHN SKWLQEHPEA GYNVETAPWL
EAALELDDAL LSYGNALQSL DLPTEFKTVE DLVSVMQQMR PHVIEKIKLW EFYSVNVQRD
TEAIIKAWKS GNLDYPKGGF GEVGVGGLSE IRQWPFEKKA AFLREKALEN DNQILGRYSR
KVNPRIGAAL ITALYGRYDE TTADIPVVEE NVFKTLDSLN LPLFREFDDD VSEIFDQLFH
RIKYLRLDDH GPKLGPVTPK NPLVETYFTR LPLNDITKAH SPRSLALVNN GWVWNADALR
DNAGPGCRAY LKREVIVWGD CVKLRYGNCP EDNPFLWDYI TRYTKLMAKH FAGFRIDNCH
STPLPVAEYL LHEARRIRPN LMVYAELFTG SEKVDYLFTK RLGLTALIRE AMQAWSAAEL
SRLVHRHGGL PIGSFDPSLL SHWCQMETKV VLHHIRPTTV HALFMDCSHD NETPAQKRDP
RDTLPNAALV AMCASAIGSV MGYDEIYPEH VNLVTETRSY HSVFSENGVQ DATGEGGIGG
IKKLLNSLHT EMAIDEYDET HIHHDAEYIT VHRVHPHTRK GTLLISRTAF SASNVKHILA
PIHLTGTKAS LLGAWKLEVE NDTEAKRIVA ADEKYLRGLP SRVSEISGFR LEENGDDTII
YTPDDFPPGS IALLKTHIHE RDLPVDLSKF ITADADATFR SVDLTGLNFT LYRCDSEERD
SSGGKDGVYK IPNFGPLVYA GLQGWWSVLE EIIKTNDLGH PLCDNLRAGQ WALDFVVGRM
RRAITQQGYS QLEGPTKWLE DRFNAVRRVP NFLLPRYFTI IIKAAYDAAC RHAIHCLGVI
IEHGQHFIHE LALVSVQHLG EVKSASLYPT NQVPCLAAGL PHFATDWARC WGRDVFISLR
GLLLATGRFD DAKEHILAFA STLKHGMIPN LLSSGKFPRY NSRDSVWFFL QAIQDYTHIV
PNGIRILEEN VPRRFLPYDD TWFPFDDKRA YSRSSTIAEI IQEVFQRHAS GISYREHNAG
PELDLQMKPE GFQVDVHVDW ETGIIFGGNQ WNCGTWMDKM GESVKAKNQG YPGTPRDGAP
IEISGLLYSA LRWVSDLRRK GHYPYSGVDI ESGLTITFDD WATRVKTHFE KCYYVPVDSE
EDRDFNVDSK LVNRRGIYKD IYKSSQPYED YQLRPNFTIA MTVAPDLFDA ERGFHALTIA
DVVLRGPLGM ATLDPTDLNY RPYYNNSEDS TDFATSKGRN YHQGPEWLWP TGYFLQALLK
FSLLRNSSHQ SLMDISQQIT MRLERCCKSL RESTWKGLTE LTNKNGEFCV DSSPTQAWSA
ACLLALYYDA SEIQKARSAS LSV
//
