ID C6B7E8_RHILS Unreviewed; 205 AA.
AC C6B7E8;
DT 01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 01-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=nitrile hydratase {ECO:0000256|ARBA:ARBA00013079};
DE EC=4.2.1.84 {ECO:0000256|ARBA:ARBA00013079};
GN OrderedLocusNames=Rleg_6981 {ECO:0000313|EMBL:ACS60006.1};
OS Rhizobium leguminosarum bv. trifolii (strain WSM1325).
OG Plasmid pR132502 {ECO:0000313|EMBL:ACS60006.1,
OG ECO:0000313|Proteomes:UP000002256}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=395491 {ECO:0000313|EMBL:ACS60006.1, ECO:0000313|Proteomes:UP000002256};
RN [1] {ECO:0000313|EMBL:ACS60006.1, ECO:0000313|Proteomes:UP000002256}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSM1325 {ECO:0000313|Proteomes:UP000002256};
RC PLASMID=Plasmid pR132502 {ECO:0000313|Proteomes:UP000002256};
RX PubMed=21304718;
RA Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K., Tiwari R.,
RA Copeland A., Nolan M., Han C., Brettin T., Land M., Ovchinikova G.,
RA Ivanova N., Mavromatis K., Markowitz V., Kyrpides N., Melino V., Denton M.,
RA Yates R., Howieson J.;
RT "Complete genome sequence of Rhizobium leguminosarum bv. trifolii strain
RT WSM1325, an effective microsymbiont of annual Mediterranean clovers.";
RL Stand. Genomic Sci. 2:347-356(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84;
CC Evidence={ECO:0000256|ARBA:ARBA00001410};
CC -!- SIMILARITY: Belongs to the nitrile hydratase subunit alpha family.
CC {ECO:0000256|ARBA:ARBA00009363}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001624; ACS60006.1; -; Genomic_DNA.
DR RefSeq; WP_003554333.1; NC_012858.1.
DR AlphaFoldDB; C6B7E8; -.
DR KEGG; rlg:Rleg_6981; -.
DR HOGENOM; CLU_092054_0_0_5; -.
DR OrthoDB; 528553at2; -.
DR Proteomes; UP000002256; Plasmid pR132502.
DR GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.90.330.10; Nitrile hydratase alpha /Thiocyanate hydrolase gamma; 1.
DR InterPro; IPR036648; CN_Hdrase_a/SCN_Hdrase_g_sf.
DR InterPro; IPR004232; CN_Hdrtase_a/SCN_Hdrlase_g.
DR InterPro; IPR023900; CN_Hdrtase_asu/SCN_Hdrlase_gsu.
DR InterPro; IPR018141; Nitrile_hydratase_asu.
DR NCBIfam; TIGR01323; nitrile_alph; 1.
DR Pfam; PF02979; NHase_alpha; 1.
DR PIRSF; PIRSF001426; NHase_alpha; 1.
DR SUPFAM; SSF56209; Nitrile hydratase alpha chain; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|PIRSR:PIRSR001426-1};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:ACS60006.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR001426-1}; Plasmid {ECO:0000313|EMBL:ACS60006.1}.
FT DOMAIN 15..200
FT /note="Nitrile hydratase alpha /Thiocyanate hydrolase
FT gamma"
FT /evidence="ECO:0000259|Pfam:PF02979"
FT BINDING 110
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
FT BINDING 113
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
FT BINDING 114
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
FT BINDING 115
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
SQ SEQUENCE 205 AA; 22746 MW; C070B55F96C391BA CRC64;
MIDRFKYRED REAYSAARVK ALEALLIKKG IITDKTVDTI LDFFETKMGP FNGAKIVARA
WLDPAFKDRL VANTPAAIAE LELPEGMAGA EGEHMRAVAN SADVHNLIIC TLCSCYPWPV
LGLPPYWYKD PTFRSRAARE PRAVLNEFGL PVPETIEIKV WDSSAQIRWF VIPERPPGTD
GLTETELEAL VTPEAMMGVA IAKAA
//
