ID C7A7X9_MALDO Unreviewed; 443 AA.
AC C7A7X9;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN Name=MAN3 {ECO:0000313|EMBL:ACN78665.1};
GN ORFNames=DVH24_031023 {ECO:0000313|EMBL:RXH68690.1};
OS Malus domestica (Apple) (Pyrus malus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX NCBI_TaxID=3750 {ECO:0000313|EMBL:ACN78665.1};
RN [1] {ECO:0000313|EMBL:ACN78665.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=19454593; DOI=10.1093/aob/mcp120;
RA Schroder R., Atkinson R.G., Redgwell R.J.;
RT "Re-interpreting the role of endo-beta-mannanases as mannan
RT endotransglycosylase/hydrolases in the plant cell wall.";
RL Ann. Bot. 104:197-204(2009).
RN [2] {ECO:0000313|EMBL:RXH68690.1, ECO:0000313|Proteomes:UP000290289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. HFTH1 {ECO:0000313|Proteomes:UP000290289};
RC TISSUE=Young leaf {ECO:0000313|EMBL:RXH68690.1};
RA Hu J.;
RT "A high-quality apple genome assembly.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; FJ194536; ACN78665.1; -; mRNA.
DR EMBL; RDQH01000343; RXH68690.1; -; Genomic_DNA.
DR RefSeq; NP_001280862.1; NM_001293933.1.
DR AlphaFoldDB; C7A7X9; -.
DR STRING; 3750.C7A7X9; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR GeneID; 103405661; -.
DR KEGG; mdm:103405661; -.
DR OrthoDB; 2717493at2759; -.
DR Proteomes; UP000290289; Chromosome 17.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451:SF45; MANNAN ENDO-1,4-BETA-MANNOSIDASE 2; 1.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 2: Evidence at transcript level;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000290289};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..37
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 121..361
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
SQ SEQUENCE 443 AA; 50925 MW; 84CD0101C1EBB7C3 CRC64;
MILGSSAEKK KMVVAGNGLF YPIIGFASCV AFIYMSFGNL GSNFGNQTKL SFVGRNGTQF
MLDGKAFYIN GWNSYWLMDN SVDEHRKPRV RQILQAGAKM GLTVCRTWAF NDGDYNALQI
SPGHFNEQVF KALDHVIVEA RQQGIRLLLC LVNNLQAYGG KTQYVKWAWE EGVGLSASND
SFFFDPSIRI YFKNYVKTLL TRKNTLNGIQ YRNDPTIFAW ELINEPRCIT DASGDTLQDW
IEEMSAFVKA IDKNHLLTVG LEGFYGPKSP KRLTVNPEMW ASRLGSDFIR NSKIPNIDFA
SVHIYPDHWF HDEEFHNNLK FVYKWMRSHI EDGDNELNKP VMFTEYGLSN QNKDFEPSQR
VRFYKTILDV IYKSAKKNQS GAGALAWQFF VGGMDEYNDE FGIIPWESPS EYKMMIEQSC
RLARLKGPTQ QQRNLKDLCS QRQ
//
