ID C7GR27_YEAS2 Unreviewed; 827 AA.
AC C7GR27;
DT 13-OCT-2009, integrated into UniProtKB/TrEMBL.
DT 13-OCT-2009, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=tRNA ligase {ECO:0000256|PIRNR:PIRNR019634};
DE EC=6.5.1.3 {ECO:0000256|PIRNR:PIRNR019634};
GN Name=TRL1 {ECO:0000313|EMBL:EEU06717.1};
GN ORFNames=C1Q_02778 {ECO:0000313|EMBL:EEU06717.1};
OS Saccharomyces cerevisiae (strain JAY291) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=574961 {ECO:0000313|EMBL:EEU06717.1, ECO:0000313|Proteomes:UP000008073};
RN [1] {ECO:0000313|EMBL:EEU06717.1, ECO:0000313|Proteomes:UP000008073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAY291 {ECO:0000313|EMBL:EEU06717.1,
RC ECO:0000313|Proteomes:UP000008073};
RX PubMed=19812109; DOI=10.1101/gr.091777.109;
RA Argueso J.L., Carazzolle M.F., Mieczkowski P.A., Duarte F.M., Netto O.V.,
RA Missawa S.K., Galzerani F., Costa G.G., Vidal R.O., Noronha M.F.,
RA Dominska M., Andrietta M.G., Andrietta S.R., Cunha A.F., Gomes L.H.,
RA Tavares F.C., Alcarde A.R., Dietrich F.S., McCusker J.H., Petes T.D.,
RA Pereira G.A.;
RT "Genome structure of a Saccharomyces cerevisiae strain widely used in
RT bioethanol production.";
RL Genome Res. 19:2258-2270(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.;
CC EC=6.5.1.3; Evidence={ECO:0000256|PIRNR:PIRNR019634};
CC -!- SIMILARITY: Belongs to the TRL1 family.
CC {ECO:0000256|PIRNR:PIRNR019634}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEU06717.1}.
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DR EMBL; ACFL01000138; EEU06717.1; -; Genomic_DNA.
DR AlphaFoldDB; C7GR27; -.
DR Proteomes; UP000008073; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051730; F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; IEA:InterPro.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR GO; GO:0003972; F:RNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR019039; T4-Rnl1-like_N.
DR InterPro; IPR012387; Trl1_fun.
DR InterPro; IPR015966; tRNA_lig_kin_fungi.
DR InterPro; IPR015965; tRNA_lig_PDEase.
DR PANTHER; PTHR32004; TRNA LIGASE; 1.
DR PANTHER; PTHR32004:SF1; TRNA LIGASE; 1.
DR Pfam; PF09511; RNA_lig_T4_1; 1.
DR Pfam; PF08302; tRNA_lig_CPD; 1.
DR Pfam; PF08303; tRNA_lig_kinase; 1.
DR PIRSF; PIRSF019634; tRNA_lig_yeast; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR019634};
KW tRNA processing {ECO:0000256|PIRNR:PIRNR019634}.
FT DOMAIN 64..292
FT /note="T4 RNA ligase 1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF09511"
FT DOMAIN 393..560
FT /note="tRNA ligase kinase"
FT /evidence="ECO:0000259|Pfam:PF08303"
FT DOMAIN 565..826
FT /note="tRNA ligase phosphodiesterase"
FT /evidence="ECO:0000259|Pfam:PF08302"
FT ACT_SITE 114
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR019634-50"
SQ SEQUENCE 827 AA; 95338 MW; 8792A3E15F1281DC CRC64;
MPSPYDGKRT VTQLVNELEK AEKLSGRGRA YRRVCDLSHS NKKVISWKFN EWDYGKNTIT
LPCNARGLFI SDDTTNPVIV ARGYDKFFNV GEVNFTKWNW IEENCTGPYD VTIKANGCII
FISGLEDGTL VVCSKHSTGP RADVDRNHAE AGEKQLLRQL AAMNINRSDF ARMLYTHNVT
AVAEYCDDSF EEHILEYPLE KAGLYLHGVN VNKAEFETWD MKDVSQMASK YGFRCVQCIT
SNTLEDLKKF LDNCSATGSF EGQEIEGFVI RCHLKSTEKP FFFKYKFEEP YLMYRQWREV
TKDYISNKSR VFKFRKHKFI TNKYLDFAIP ILESSPEICE NYLKGFGVIE LRNKFLQSYG
MSGLEILNHE KVAELELKNA IDYDKVDERT KFLIFPISVI GCGKTTTSQT LVNLFPDSWG
HIQNDDITGK DKSQLMKKSL ELLSKKEIKC VIVDRNNHQF RERKQLFEWL NELKEDYLVY
DTNIKVIGVS FAPYDKLSEI RDITLQRVIK RGNNHQSIKW DELGEKKVVG IMNGFLKRYQ
PVNLDKSPDN MFDLMIELDF GQADSSLTNA KQILNEIHKA YPILVPEIPK DDEIETAFRR
SLDYKPTVRK IVGKGNNNQQ KTPKLIKPTY ISAKIENYDE IIELVKRCIA SDAELTEKFK
HLLASGKVQK ELHITLGHVM SSREKEAKKL WKSYCNRYTD QITEYNNNRI ENAQGSGNNQ
NTQVKTTDKL NFRLEKLCWD EKIIAIVVEL SKDKDGCIID ENNEKIKGLC CQNKIPHITL
CKLESGVKAV YSNVLCEKVE SAEVDENIKV VKLDNSKEFV GSVYLNF
//
