ID CG121_METJA Reviewed; 145 AA.
AC Q57646;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE RecName: Full=Regulatory protein Cgi121;
DE AltName: Full=Positive regulator of Bud32 kinase activity;
GN Name=cgi121; OrderedLocusNames=MJ0187;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3.60 ANGSTROMS) IN COMPLEX WITH KAE1-BUD32,
RP STRUCTURE BY NMR, FUNCTION, SUBUNIT, AND MUTAGENESIS OF LEU-134; ALA-138
RP AND LEU-139.
RX PubMed=18951093; DOI=10.1016/j.molcel.2008.10.002;
RA Mao D.Y., Neculai D., Downey M., Orlicky S., Haffani Y.Z., Ceccarelli D.F.,
RA Ho J.S., Szilard R.K., Zhang W., Ho C.S., Wan L., Fares C., Rumpel S.,
RA Kurinov I., Arrowsmith C.H., Durocher D., Sicheri F.;
RT "Atomic structure of the KEOPS complex: an ancient protein kinase-
RT containing molecular machine.";
RL Mol. Cell 32:259-275(2008).
CC -!- FUNCTION: Component of the KEOPS complex that is probably involved in
CC the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP
CC (TC-AMP) to the N6 group of A37, a step in the formation of a
CC threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs
CC that read codons beginning with adenine. Cgi121 stimulates Bud32 kinase
CC activity via an activation of Bud32 autophosphorylation.
CC {ECO:0000269|PubMed:18951093}.
CC -!- SUBUNIT: Component of the KEOPS complex that consists of Kae1, Bud32,
CC Cgi121 and Pcc1; the whole complex dimerizes.
CC {ECO:0000269|PubMed:18951093}.
CC -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR EMBL; L77117; AAB98167.1; -; Genomic_DNA.
DR PIR; D64323; D64323.
DR RefSeq; WP_010869682.1; NC_000909.1.
DR PDB; 2K8Y; NMR; -; A=1-145.
DR PDB; 3ENH; X-ray; 3.60 A; C/D=1-145.
DR PDB; 7KJT; X-ray; 3.34 A; H=1-145.
DR PDBsum; 2K8Y; -.
DR PDBsum; 3ENH; -.
DR PDBsum; 7KJT; -.
DR AlphaFoldDB; Q57646; -.
DR BMRB; Q57646; -.
DR SMR; Q57646; -.
DR STRING; 243232.MJ_0187; -.
DR PaxDb; 243232-MJ_0187; -.
DR EnsemblBacteria; AAB98167; AAB98167; MJ_0187.
DR GeneID; 1451034; -.
DR KEGG; mja:MJ_0187; -.
DR eggNOG; arCOG02197; Archaea.
DR HOGENOM; CLU_1801715_0_0_2; -.
DR InParanoid; Q57646; -.
DR OrthoDB; 69587at2157; -.
DR PhylomeDB; Q57646; -.
DR EvolutionaryTrace; Q57646; -.
DR Proteomes; UP000000805; Chromosome.
DR Gene3D; 3.30.2380.10; CGI121/TPRKB; 1.
DR InterPro; IPR013926; CGI121/TPRKB.
DR InterPro; IPR036504; CGI121/TPRKB_sf.
DR Pfam; PF08617; CGI-121; 1.
DR SUPFAM; SSF143870; PF0523-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..145
FT /note="Regulatory protein Cgi121"
FT /id="PRO_0000106735"
FT MUTAGEN 134
FT /note="L->E: No effect on Bud32 binding."
FT /evidence="ECO:0000269|PubMed:18951093"
FT MUTAGEN 134
FT /note="L->E: Severely affects Bud32 binding and does not
FT stimulate Bud32 kinase activity; when associated with R-
FT 138."
FT /evidence="ECO:0000269|PubMed:18951093"
FT MUTAGEN 138
FT /note="A->R: No effect on Bud32 binding."
FT /evidence="ECO:0000269|PubMed:18951093"
FT MUTAGEN 138
FT /note="A->R: Severely affects Bud32 binding and does not
FT stimulate Bud32 kinase activity; when associated with E-
FT 134."
FT /evidence="ECO:0000269|PubMed:18951093"
FT MUTAGEN 139
FT /note="L->R: No effect on Bud32 binding."
FT /evidence="ECO:0000269|PubMed:18951093"
FT STRAND 2..9
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 13..17
FT /evidence="ECO:0007829|PDB:7KJT"
FT STRAND 21..26
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 33..45
FT /evidence="ECO:0007829|PDB:7KJT"
FT STRAND 49..52
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 53..62
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 67..74
FT /evidence="ECO:0007829|PDB:7KJT"
FT STRAND 78..87
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 88..98
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 104..107
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 111..120
FT /evidence="ECO:0007829|PDB:7KJT"
FT STRAND 125..127
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 129..138
FT /evidence="ECO:0007829|PDB:7KJT"
FT HELIX 139..141
FT /evidence="ECO:0007829|PDB:7KJT"
SQ SEQUENCE 145 AA; 16593 MW; E7CB7330D1E1003F CRC64;
MIIRGIRGAR INNEIFNLGL KFQILNADVV ATKKHVLHAI NQAKTKKPIA KSFWMEILVR
ASGQRQIHEA IKIIGAKDGN VCLICEDEET FRKIYELIGG EIDDSVLEIN EDKERLIREI
FKIRGFGNVV ERVLEKIALI ELKKE
//
