ID CHS1_CICAR Reviewed; 389 AA.
AC Q9SML4;
DT 08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 83.
DE RecName: Full=Chalcone synthase 1;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase 1;
GN Name=CHS1;
OS Cicer arietinum (Chickpea) (Garbanzo).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX NCBI_TaxID=3827;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. ILC 3279; TISSUE=Leaf;
RA Hanselle T., Schwenger-Erger C., Barz W.;
RT "Isolation of a full length chalcone synthase cDNA from infected chickpea
RT plants (Cicer arietinum L.).";
RL (er) Plant Gene Register PGR99-100(1999).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ012822; CAA10190.1; -; mRNA.
DR AlphaFoldDB; Q9SML4; -.
DR SMR; Q9SML4; -.
DR STRING; 3827.Q9SML4; -.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000087171; Genome assembly.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF62; CHALCONE SYNTHASE 7; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase 1"
FT /id="PRO_0000215967"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 389 AA; 42874 MW; 04CB0D50086168AC CRC64;
MVSVSEIRKA QRAEGPATIL AIGTANPSNR VEQSTYPDFY FKITNSEHKV ELKQKFQRMC
DKSMIKSRYM YLTEEILKEN PSVCEYMAPS LDVRQDMVVV EVPRLGKEAA VKAIKEWGQP
KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPIP EIEKPIFEMV
WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNID KALIEAFQPL NISDYNSIFW
IAHPGGPAIL DQVEEKLALK PEKMRATREV LSEYGNMSSA CVLFILDEMR RKSAKDGLKT
TGEGLEWGVL FGFGPGLTIE TVVLHSVAI
//
