ID CHS5_MEDSA Reviewed; 389 AA.
AC P51078;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 22-FEB-2023, entry version 89.
DE RecName: Full=Chalcone synthase 4-2;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase 4-2;
GN Name=CHS4-2; Synonyms=CHSI;
OS Medicago sativa (Alfalfa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3879;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nagyszenasi; TISSUE=Leaf;
RX PubMed=8274775; DOI=10.1094/mpmi-6-655;
RA Esnault R., Buffard D., Breda C., Sallaud C., El-Turk J., Kondorosi A.;
RT "Pathological and molecular characterizations of alfalfa interactions with
RT compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae
RT and Pseudomonas syringae pv. pisi.";
RL Mol. Plant Microbe Interact. 6:655-664(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 19-389.
RC STRAIN=cv. Iroquois; TISSUE=Root nodule;
RX PubMed=8193301; DOI=10.1007/bf00029858;
RA McKhann H.I., Hirsch A.M.;
RT "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa
RT (Medicago sativa L.): highest transcript levels occur in young roots and
RT root tips.";
RL Plant Mol. Biol. 24:767-777(1994).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- DEVELOPMENTAL STAGE: Highest expression in young root tips.
CC -!- INDUCTION: By infection.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; X68106; CAA48226.1; -; mRNA.
DR EMBL; U01019; AAB41560.1; -; mRNA.
DR PIR; S26414; S26414.
DR AlphaFoldDB; P51078; -.
DR SMR; P51078; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF62; CHALCONE SYNTHASE 7; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..389
FT /note="Chalcone synthase 4-2"
FT /id="PRO_0000216010"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT CONFLICT 86
FT /note="I -> Y (in Ref. 2; AAB41560)"
FT /evidence="ECO:0000305"
FT CONFLICT 94
FT /note="W -> R (in Ref. 2; AAB41560)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 389 AA; 42661 MW; B33674EC7709F170 CRC64;
MVSVSEIRNA QRAEGPATTL AIGTANPTNC VEQSTYPDFY FKITNSEHKT ELKEKFQRMC
DKSMIKRRYM YLTEEILKEN PSVCEIMAPS LDAWQDMVVV EVPRLGKEAA VKAIKEWGQP
KSKITHLIVC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN
NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV
WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNIN KALVEAFEPL GISDYNSIFW
IAHPGGPAIL DQVEQKLALK PEKMKATREV LSEYGNMSSA CVLFILDEMR KKSAQDGLKT
TGEGLEWGVL FGFGPGLTIE TVVLRSVAI
//
