ID CHSY_ARAAL Reviewed; 391 AA.
AC Q9SEP4;
DT 25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 84.
DE RecName: Full=Chalcone synthase;
DE EC=2.3.1.74;
DE AltName: Full=Naringenin-chalcone synthase;
GN Name=CHS;
OS Arabis alpina (Alpine rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis.
OX NCBI_TaxID=50452;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AlpAF;
RX PubMed=11018155; DOI=10.1093/oxfordjournals.molbev.a026248;
RA Koch M.A., Haubold B., Mitchell-Olds T.;
RT "Comparative evolutionary analysis of chalcone synthase and alcohol
RT dehydrogenase loci in Arabidopsis, Arabis, and related genera
RT (Brassicaceae).";
RL Mol. Biol. Evol. 17:1483-1498(2000).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AF112083; AAF23558.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9SEP4; -.
DR SMR; Q9SEP4; -.
DR eggNOG; ENOG502S4A6; Eukaryota.
DR UniPathway; UPA00154; -.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:EnsemblPlants.
DR GO; GO:0005634; C:nucleus; IEA:EnsemblPlants.
DR GO; GO:0009705; C:plant-type vacuole membrane; IEA:EnsemblPlants.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009926; P:auxin polar transport; IEA:EnsemblPlants.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0031540; P:regulation of anthocyanin biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0009733; P:response to auxin; IEA:EnsemblPlants.
DR GO; GO:0009629; P:response to gravity; IEA:EnsemblPlants.
DR GO; GO:0009753; P:response to jasmonic acid; IEA:EnsemblPlants.
DR GO; GO:0006979; P:response to oxidative stress; IEA:EnsemblPlants.
DR GO; GO:0010224; P:response to UV-B; IEA:EnsemblPlants.
DR GO; GO:0009611; P:response to wounding; IEA:EnsemblPlants.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Flavonoid biosynthesis; Transferase.
FT CHAIN 1..391
FT /note="Chalcone synthase"
FT /id="PRO_0000215953"
FT ACT_SITE 165
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ SEQUENCE 391 AA; 42658 MW; 103B92D7BDB20001 CRC64;
MAPSLEEIRK AQRADGPAGI LGIGTANPPN HVLQAEYPDY YFRITNSDHM TDLKEKFKRM
CDKSMIRKRH MHLTEEFLKE NPKMCAYMAP SLDTRQDIVV VEVPKLGKEA AVKAIKEWGQ
PKSKITHVVF CTTSGVDMPG ADYQLTKLLG LRPSVKRLMM YQQGCFAGGT VLRLAKDLAE
NNRGARVLVV CSEITAVTFR GPSDTHLDSL VGQALFSDGA AALIVGSDPD TSVGEKPIFE
MVSAAQTILP DSDGAIDGHL REVGLTFHLL KDVPGLISKN IEKSLDEAFK PLGISDWNSL
FWIAHPGGPA ILDQVEIKLG LKAEKMRATR HVLSEYGNMS SACVLFILDE MRKKSAKDGA
ATTGEGLEWG VLFGFGPGLT VETVVLHSVP L
//
