ID D1KS62_PANSA Unreviewed; 520 AA.
AC D1KS62;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=Nitrogenase molybdenum-iron protein beta chain {ECO:0000256|ARBA:ARBA00014775, ECO:0000256|RuleBase:RU364127};
DE EC=1.18.6.1 {ECO:0000256|ARBA:ARBA00012773, ECO:0000256|RuleBase:RU364127};
DE AltName: Full=Dinitrogenase {ECO:0000256|RuleBase:RU364127};
GN Name=nifK {ECO:0000313|EMBL:ACU32752.1};
GN OrderedLocusNames=Pat9b_4718 {ECO:0000313|EMBL:ADU72687.1};
OS Pantoea sp. (strain At-9b).
OG Plasmid pPAT9B03 {ECO:0000313|EMBL:ADU72687.1,
OG ECO:0000313|Proteomes:UP000001624}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea.
OX NCBI_TaxID=592316 {ECO:0000313|EMBL:ACU32752.1};
RN [1] {ECO:0000313|EMBL:ACU32752.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=At-9b {ECO:0000313|EMBL:ACU32752.1};
RA Pinto-Thomas A.A., Anderson M.A., Suen G., Stevenson D.M., Chu F.S.T.,
RA Cleland W.W., Weimer P.J., Currie C.R.;
RT "Symbiotic Nitrogen Fixation in the Fungus Gardens of Leaf-Cutter Ants.";
RL Science 326:1120-1123(2009).
RN [2] {ECO:0000313|EMBL:ADU72687.1, ECO:0000313|Proteomes:UP000001624}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=At-9b {ECO:0000313|Proteomes:UP000001624};
RC PLASMID=At-9b {ECO:0000313|EMBL:ADU72687.1}, Plasmid pPAT9B03
RC {ECO:0000313|Proteomes:UP000001624}, and pPAT9B03
RC {ECO:0000313|EMBL:ADU72687.1};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.;
RT "Complete sequence plasmid3 of Pantoea sp. At-9b.";
RL Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation. {ECO:0000256|ARBA:ARBA00002621,
CC ECO:0000256|RuleBase:RU364127}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000805,
CC ECO:0000256|RuleBase:RU364127};
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143;
CC Evidence={ECO:0000256|RuleBase:RU364127};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer.
CC {ECO:0000256|RuleBase:RU364127};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC {ECO:0000256|ARBA:ARBA00011462, ECO:0000256|RuleBase:RU364127}.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC {ECO:0000256|ARBA:ARBA00011002, ECO:0000256|RuleBase:RU004021}.
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DR EMBL; GQ342604; ACU32752.1; -; Genomic_DNA.
DR EMBL; CP002436; ADU72687.1; -; Genomic_DNA.
DR RefSeq; WP_013512512.1; NC_014840.1.
DR AlphaFoldDB; D1KS62; -.
DR KEGG; pao:Pat9b_4718; -.
DR HOGENOM; CLU_025876_2_0_6; -.
DR OrthoDB; 9800746at2; -.
DR Proteomes; UP000001624; Plasmid pPAT9B03.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01974; Nitrogenase_MoFe_beta; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 3.
DR Gene3D; 1.20.89.10; Nitrogenase Molybdenum-iron Protein, subunit B, domain 4; 1.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR NCBIfam; TIGR01286; nifK; 1.
DR PANTHER; PTHR33712; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT B; 1.
DR PANTHER; PTHR33712:SF7; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT B; 1.
DR Pfam; PF11844; DUF3364; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU364127};
KW Iron {ECO:0000256|RuleBase:RU364127};
KW Iron-sulfur {ECO:0000256|RuleBase:RU364127};
KW Metal-binding {ECO:0000256|RuleBase:RU364127};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU004021};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU364127};
KW Oxidoreductase {ECO:0000256|RuleBase:RU364127,
KW ECO:0000313|EMBL:ADU72687.1}; Plasmid {ECO:0000313|EMBL:ADU72687.1}.
FT DOMAIN 1..55
FT /note="Nitrogenase molybdenum-iron protein beta chain N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF11844"
FT DOMAIN 69..498
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
SQ SEQUENCE 520 AA; 58526 MW; 395D8E2B443F609E CRC64;
MSQTVDKIHN CHPLFEQDEY QKLFSGKRSQ EEAWDQARVQ EVFQWTTTAE YEALNFKREA
LTIDPAKACQ PLGAVLCSLG FANTLPYVHG SQGCVAYFRT YFNRHFKEPV ACVSDSMTED
AAVFGGNNNM NTGLQNASIL YKPEIIAVST TCMAEVIGDD LQAFIANAKK DGFVDASLPV
PFAHTPSFIG SHITGWDNMF EGFAKTFTAD HEAQPGRIPR LNMVTGFETY LGNFRVLKRM
MAQMAVPCSL LSDPSEVLDT PADGHYHMYA GGTTQQEMRE APDAIDTLLL QPWQLVKSKK
VVQEMWNQPA TEVQIPMGLA ATDDLLMTVS QLTGTAIPDV LALERGRLVD MMLDSHSWLH
GKKFGLFGDP DFVMGLTRFL LELGCEPTVV LCHNGSKRWQ KAMKKMLETS PYGKESEVFI
NCDLWHFRSL MFTRQPDFMI GNSYAKFIQR DTLAKGEQFE VPLIRLGFPL FDRHHLHRQT
TWGYEGAMSM LTTLVNAVLE KVDKDTIKLG KTDYGFDLIR
//
