ID D2CNA7_NODSP Unreviewed; 511 AA.
AC D2CNA7;
DT 09-FEB-2010, integrated into UniProtKB/TrEMBL.
DT 09-FEB-2010, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Nitrogenase molybdenum-iron protein beta chain {ECO:0000256|ARBA:ARBA00014775, ECO:0000256|RuleBase:RU364127};
DE EC=1.18.6.1 {ECO:0000256|ARBA:ARBA00012773, ECO:0000256|RuleBase:RU364127};
DE AltName: Full=Dinitrogenase {ECO:0000256|RuleBase:RU364127};
GN Name=nifK {ECO:0000313|EMBL:ACA61798.1};
OS Nodularia spumigena PCC 73104.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Aphanizomenonaceae;
OC Nodularia.
OX NCBI_TaxID=128405 {ECO:0000313|EMBL:ACA61798.1};
RN [1] {ECO:0000313|EMBL:ACA61798.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PCC 73104 {ECO:0000313|EMBL:ACA61798.1};
RX PubMed=20640414; DOI=10.1007/s00239-010-9365-8;
RA Hartmann L.S., Barnum S.R.;
RT "Inferring the evolutionary history of Mo-dependent nitrogen fixation from
RT phylogenetic studies of nifK and nifDK.";
RL J. Mol. Evol. 71:70-85(2010).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation. {ECO:0000256|ARBA:ARBA00002621,
CC ECO:0000256|RuleBase:RU364127}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000805,
CC ECO:0000256|RuleBase:RU364127};
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143;
CC Evidence={ECO:0000256|RuleBase:RU364127};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer.
CC {ECO:0000256|RuleBase:RU364127};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC {ECO:0000256|ARBA:ARBA00011462, ECO:0000256|RuleBase:RU364127}.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC {ECO:0000256|ARBA:ARBA00011002, ECO:0000256|RuleBase:RU364127}.
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DR EMBL; EU358084; ACA61798.1; -; Genomic_DNA.
DR AlphaFoldDB; D2CNA7; -.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01974; Nitrogenase_MoFe_beta; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 3.
DR Gene3D; 1.20.89.10; Nitrogenase Molybdenum-iron Protein, subunit B, domain 4; 1.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR NCBIfam; TIGR01286; nifK; 1.
DR PANTHER; PTHR33712; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT B; 1.
DR PANTHER; PTHR33712:SF7; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT B; 1.
DR Pfam; PF11844; DUF3364; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU364127};
KW Iron {ECO:0000256|RuleBase:RU364127};
KW Iron-sulfur {ECO:0000256|RuleBase:RU364127};
KW Metal-binding {ECO:0000256|RuleBase:RU364127};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU364127};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU364127};
KW Oxidoreductase {ECO:0000256|RuleBase:RU364127}.
FT DOMAIN 1..55
FT /note="Nitrogenase molybdenum-iron protein beta chain N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF11844"
FT DOMAIN 69..489
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
SQ SEQUENCE 511 AA; 57445 MW; 1CA6D3F354086C53 CRC64;
MPQNPENIQD HVELFRQPEY KQLFQNKKEF ENGHDPAEVE RVAEWTKGWE YREKNFAREA
LTVNPAKGCQ PLGAIFAAVG FEATMPFVQG SQGCVAYFRT HLTRHYKEPF SGVSSSMTED
AAVFGGLQNM IDGLANSYQL YKPKMIAVCT TCMAEVIGDD LQSFINNAKE AGSVPQTFPV
PYAHTPSFVG SHITGYDNMM KGILSNLTAG QKKATSNGKI NFIPGFDTYV GNNREIKRIS
SLFGFDYTIL ADNSDYLDSP NTGEFDMYPG GTKLEDAADS INGKVTIALQ AYSTTKTREY
IEKEWKQPTV VSRPWGIKGT DEFLMKLSEL SGTAIPEELE IERGRAVDAM TDSHSWIHGK
RFAIFGEPDL VISVVGFMLE MGAEPVHILV NNSNEVFEKE LQELLDSSPF GQGATIWGGK
DLWHLRSLLF TEPVDLLIGN SYGKYLWRDC KVPFVRIGYP IMDRHHLHRY STIGYQGIIN
LLNWIVNTIF EDIDRNTNIP SKTDISFDLI R
//
