ID D2IE14_9PICO Unreviewed; 2179 AA.
AC D2IE14;
DT 09-FEB-2010, integrated into UniProtKB/TrEMBL.
DT 09-FEB-2010, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=Genome polyprotein {ECO:0000256|ARBA:ARBA00020107};
OS Human parechovirus 1.
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Picornaviridae; Paavivirinae; Parechovirus; Parechovirus A.
OX NCBI_TaxID=12063 {ECO:0000313|EMBL:ADA79684.1, ECO:0000313|Proteomes:UP000103687};
RN [1] {ECO:0000313|EMBL:ADA79684.1, ECO:0000313|Proteomes:UP000103687}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K150-93 {ECO:0000313|EMBL:ADA79684.1};
RX PubMed=19759239; DOI=10.1099/vir.0.014670-0;
RA Benschop K.S., de Vries M., Minnaar R.P., Stanway G., van der Hoek L.,
RA Wolthers K.C., Simmonds P.;
RT "Comprehensive full-length sequence analyses of human parechoviruses:
RT diversity and recombination.";
RL J. Gen. Virol. 91:145-154(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004180}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004180}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004180}. Host cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004295}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004295}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004295}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
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DR EMBL; GQ183023; ADA79684.1; -; Genomic_RNA.
DR Proteomes; UP000103687; Genome.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039618; C:T=pseudo3 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0005216; F:monoatomic ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0051259; P:protein complex oligomerization; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0039707; P:virus-mediated pore formation in host cell membrane; IEA:UniProtKB-KW.
DR CDD; cd23217; Parechovirus_RdRp; 1.
DR CDD; cd00205; rhv_like; 2.
DR Gene3D; 2.60.120.20; -; 3.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR004004; Helic/Pol/Pept_Calicivir-typ.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR007053; LRAT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR000199; Peptidase_C3A/C3B_picornavir.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001676; Picornavirus_capsid.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR033703; Rhv-like.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR029053; Viral_coat.
DR InterPro; IPR009419; VPP_parechovir_P3A.
DR InterPro; IPR009407; VPP_parechovir_P3B.
DR Pfam; PF06344; Parecho_VpG; 1.
DR Pfam; PF00548; Peptidase_C3; 1.
DR Pfam; PF06363; Picorna_P3A; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00073; Rhv; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR PRINTS; PR00918; CALICVIRUSNS.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF88633; Positive stranded ssRNA viruses; 3.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS51934; LRAT; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Capsid protein {ECO:0000256|ARBA:ARBA00022561};
KW Covalent protein-RNA linkage {ECO:0000256|ARBA:ARBA00022520};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00022488};
KW Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW T=pseudo3 icosahedral capsid protein {ECO:0000256|ARBA:ARBA00022706};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transport {ECO:0000256|ARBA:ARBA00022448};
KW Viral attachment to host cell {ECO:0000256|ARBA:ARBA00022804};
KW Viral ion channel {ECO:0000256|ARBA:ARBA00023039};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Virion {ECO:0000256|ARBA:ARBA00022706};
KW Virus entry into host cell {ECO:0000256|ARBA:ARBA00023296}.
FT DOMAIN 786..880
FT /note="LRAT"
FT /evidence="ECO:0000259|PROSITE:PS51934"
FT DOMAIN 1156..1317
FT /note="SF3 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51218"
FT DOMAIN 1517..1707
FT /note="Peptidase C3"
FT /evidence="ECO:0000259|PROSITE:PS51874"
FT DOMAIN 1944..2058
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 2179 AA; 245588 MW; 110E4C90C65E5316 CRC64;
METIKSIADM ATGVVSSVDS TINAVNEKVE NVGNEIGGNL LTKVADDASN VLGPNCYATT
AEPENKDVVQ ATTTVNTTNL TQHPSAPTMP FTPDFSNVDT FHSMAYDITT GEKNPSKLVR
LETHEWTPTW ARGHQITHVE LPKVFWDKQS KPAYGQSRYF AAVRCGFHFQ VQVNVNQGTA
GSALVVYEPK PVVTYDTKLE FGAFTNLPHV LMNLAETTQA DLCIPYVADT NYVKTDSSDL
GQLKVYVWTP LSIPSGSATQ VDVTILGSLL QLDFQNPRVF GQDVGIYDNA PTRKQNLKKI
LTMSTKYKWT RQKIDIAEGP GSMNMANVLS TTAAQSIALV GERAFYDPRT AGSKSRFDDL
VKISQLFSVM SDSTTPSANH GIDAKGYFKW SSTTAPQSVV HRNIVYLKLF PNLNVFVNSY
SYFRGSIVLR LSVYASTFNR GRLRMGFFPN ATEDSTSEID NAIYTICDLG SDNSFEITIP
YSFSTWMRKT DGHPIGLFQI EVLNRLTYNS SSPNEVYCIV QGKMGQDARF FCPTGSVVTF
QNSWGSQMDL TDPLCLEESA EECKQTISPN ELGLTSAQDD GPLGDNKPNY FLNFKSMNVD
IFTVSHTKVD NLFGRAWFYQ EHTFTNEGQW RVNLEFPKQG HGSLSLLFAY FTGELNIHVL
FLAEKGFLRV AHTYDTSENR VAFLSSNGVI TIPAGEQMTL SAPYYSNKPL RTVRDSNSLG
YLMCKPFLTG TTTGKIEVYL SLRCPNFFFP LPAPKVTTGR ALRGDLANFS DQSPYDQQLQ
SQVMKLAYLD RGFYKHYGII VGESVYQLDS DDIFKTALTG KAKFTKTKLT PDWVIEEECE
LDYFRVKYLE SSVNSEHIFS VDHNCETIAK DIFGTHTISQ HQAVGLIGTI LLTAGLMSTI
KTPVNATTIK EFFNHAVEGD EQGLSLLVQK CTTFFSSAAT EILDNDLVKF IVKILVRILC
YMVLYCHKPN ILTTACLSTL LIMDVTSSSV LSPSCKALMQ CLMDGDVKKL AEVVAESMSN
TDDEEIKEQI CDTVKYTISI ISNQGPFKGF NKVSTAFRHI DWWIHTLLKI KDMVMSVFKP
SMENKAIQWL ERNKEHVCAI LDYASDIIVE SKDQTKMKSQ EFYQKYTDCL SKFKPIMAIC
FRSCHNSISN TVYRLFQELA RIPSRISTQN DLIRVEPIGV WIQGEPGQGK SFLTHTLSRQ
LQKSCKLNGV YTNPTASEFM DGYDNQDIHL IDDLGQTRKE KDIEMLCNCI SSVPFIVPMA
HLEEKGKFYT SKLVIATTNK SDFSSTVLQD SGALKRRFPY IMHIRAAKAY SKAGKLNVSQ
AMSIMATGEC WEVSKNGRDW ETLKLKDLVN KITEDYQERQ KNYDCWKQQL ENQTLDDLDD
AVSFIKHNFP DAIPYIDEYL NIEMSTLIEQ MEAFIEPRPS VFKCFATKVA NQTRKAAKEV
VEWFSSKIKS MLSFVERNKA WLTVVSAVTS AISILLLVTK IFKKEGSKDE RAYNPTLPVT
KPKGTFPVAQ REFKNEAPYD GQLEHIISQS AYITGSTTGH LTHCAGYQHD EIILHGHSIK
YLEQEEELTL HYKNKVFPID CPSVTQVTLG GKPMDLAILK CKLPFRFKKN SKYYTNKIGT
ESMLIWMTEQ GIITKEVQRV HHSGGIKTRE GTESTKTISY TVKSCKGMCG GLLISKVEGN
FKILGMHIAG NGEMGVAIPF NFLKNDMSDQ GIVTEVTPIQ PMYINTKTQI HKSPVYGAVE
VKMGPAVLSK SDPRLEEPVE CLIKKSAAKY RVNKFQVNNE LWQGVKACVK SKFREIFGIN
GIVDMKTAIL GTSHVNSMDL STSAGYSFVK SGYKKKDLIC LEPFYIHPML EKLVQEKFHN
LLKGNQITTI FNTCLKDELR KLDKISAGKT RCIEACEVDY CIVYRMIMME IYDKIYQTPC
YYSGLAVGIN PYKDWHFMIN ALNDYNYEMD YSQYDGSLSS MLLWEAVEVL AYCHDSPDLV
MQLHKPVIDS DHVVFNERWM IHGGMPSGSP CTTVLNSLCN LMMCIYTTNL ISPGIDCLPI
VYGDDVILSL DKEIDPEKLQ SIMADSFGAE VTGSRKDEPP SLKPRLEVEF LKRKPGYFPE
STFIVGKLDT ENMIQHLMWM KNFSTFKQQL QSYLMELCLH GKDTYLHYIK ILDPYLKEWN
ITVDDYDVVV AKLMPMVFD
//
