ID D2TM05_CITRI Unreviewed; 578 AA.
AC D2TM05;
DT 02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 02-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE RecName: Full=K(+)/H(+) antiporter NhaP2 {ECO:0000256|HAMAP-Rule:MF_01075};
DE AltName: Full=Potassium/proton antiporter NhaP2 {ECO:0000256|HAMAP-Rule:MF_01075};
GN Name=nhaP2 {ECO:0000256|HAMAP-Rule:MF_01075};
GN Synonyms=cvrA {ECO:0000313|EMBL:CBG88582.1};
GN OrderedLocusNames=ROD_18271 {ECO:0000313|EMBL:CBG88582.1};
OS Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Citrobacter.
OX NCBI_TaxID=637910 {ECO:0000313|EMBL:CBG88582.1, ECO:0000313|Proteomes:UP000001889};
RN [1] {ECO:0000313|EMBL:CBG88582.1, ECO:0000313|Proteomes:UP000001889}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ICC168 {ECO:0000313|EMBL:CBG88582.1,
RC ECO:0000313|Proteomes:UP000001889};
RX PubMed=19897651; DOI=10.1128/JB.01144-09;
RA Petty N.K., Bulgin R., Crepin V.F., Cerdeno-Tarraga A.M., Schroeder G.N.,
RA Quail M.A., Lennard N., Corton C., Barron A., Clark L., Toribio A.L.,
RA Parkhill J., Dougan G., Frankel G., Thomson N.R.;
RT "The Citrobacter rodentium genome sequence reveals convergent evolution
RT with human pathogenic Escherichia coli.";
RL J. Bacteriol. 192:525-538(2010).
CC -!- FUNCTION: K(+)/H(+) antiporter that extrudes potassium in exchange for
CC external protons and maintains the internal concentration of potassium
CC under toxic levels. {ECO:0000256|HAMAP-Rule:MF_01075}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01075};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01075}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01075}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. NhaP2 subfamily. {ECO:0000256|HAMAP-
CC Rule:MF_01075}.
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DR EMBL; FN543502; CBG88582.1; -; Genomic_DNA.
DR RefSeq; WP_012906064.1; NC_013716.1.
DR AlphaFoldDB; D2TM05; -.
DR STRING; 637910.ROD_18271; -.
DR KEGG; cro:ROD_18271; -.
DR eggNOG; COG3263; Bacteria.
DR HOGENOM; CLU_005912_9_2_6; -.
DR OrthoDB; 9810759at2; -.
DR Proteomes; UP000001889; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006884; P:cell volume homeostasis; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR Gene3D; 3.30.465.10; -; 1.
DR Gene3D; 3.30.70.1450; Regulator of K+ conductance, C-terminal domain; 1.
DR HAMAP; MF_01075; NhaP2; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR023729; NhaP2.
DR InterPro; IPR006037; RCK_C.
DR InterPro; IPR036721; RCK_C_sf.
DR InterPro; IPR005170; Transptr-assoc_dom.
DR PANTHER; PTHR32507:SF7; K(+)_H(+) ANTIPORTER NHAP2; 1.
DR PANTHER; PTHR32507; NA(+)/H(+) ANTIPORTER 1; 1.
DR Pfam; PF03471; CorC_HlyC; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR Pfam; PF02080; TrkA_C; 1.
DR SMART; SM01091; CorC_HlyC; 1.
DR SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR SUPFAM; SSF116726; TrkA C-terminal domain-like; 1.
DR PROSITE; PS51202; RCK_C; 1.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|ARBA:ARBA00022449, ECO:0000256|HAMAP-Rule:MF_01075};
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01075};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_01075};
KW Potassium {ECO:0000256|HAMAP-Rule:MF_01075};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_01075};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|HAMAP-Rule:MF_01075}.
FT TRANSMEM 6..23
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 30..47
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 59..76
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 88..115
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 185..209
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 221..238
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 244..261
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 273..292
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 298..323
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 335..357
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT TRANSMEM 363..381
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01075"
FT DOMAIN 403..485
FT /note="RCK C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51202"
SQ SEQUENCE 578 AA; 62208 MW; 8DE6B6C94093B872 CRC64;
MDATAIISLF ILGSVLVTSS ILLSSFSSRL GIPILVIFLA IGMLAGVDGV GGIPFDNYPF
AWMVSNLALA IILLDGGMRT QASSFRVALG PALSLATVGV LITSGLTGMM AAWLFNLDLM
EGLLIGAIVG STDAAAVFSL LGGKGLNERV GSTLEIESGS NDPMAVFLTI TLIEMIQKHQ
TGLDWMFAVH IVQQFGLGIV LGLGGGYLLQ QMINRISLPG GLYPLLALSG GILIFALTTA
LDGSGILAVY LCGFLLGNRP IRNRYAILQN FDGLAWLAQI AMFLVLGLLV TPSDLLPIVV
PALILSAWMI FFARPVSVFA GLLPFRGFNL RERVFISWVG LRGAVPIILA VFPMMAGLEN
ARLFFNVAFF VVLISLLFQG TSLSWAAKKA KVVVPPVGWP VSRVGLDIHP DNPWEQFVYQ
LSADKWCVGA QLRDLHMPEE TRIAALFRDN VLCHPTGSTR LREGDVLCVI GRERDLPALG
KLFSQSPPVA LDQRFFGDFI LEASAKFADV ALIYGLEEGA DYRDKQQTLG EIVQQLLGAA
PVVGDQVEFA GMIWTVAEKE DNAVRKVGVR VAGEDDEE
//
