ID   D2V4D2_NAEGR            Unreviewed;       915 AA.
AC   D2V4D2;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 56.
DE   SubName: Full=RasGEF domain-containing protein {ECO:0000313|EMBL:EFC48495.1};
GN   ORFNames=NAEGRDRAFT_78524 {ECO:0000313|EMBL:EFC48495.1};
OS   Naegleria gruberi (Amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5762 {ECO:0000313|Proteomes:UP000006671};
RN   [1] {ECO:0000313|EMBL:EFC48495.1, ECO:0000313|Proteomes:UP000006671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEG-M {ECO:0000313|EMBL:EFC48495.1,
RC   ECO:0000313|Proteomes:UP000006671};
RX   PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA   Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA   Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA   Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA   Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT   "The genome of Naegleria gruberi illuminates early eukaryotic
RT   versatility.";
RL   Cell 140:631-642(2010).
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DR   EMBL; GG738851; EFC48495.1; -; Genomic_DNA.
DR   RefSeq; XP_002681239.1; XM_002681193.1.
DR   AlphaFoldDB; D2V4D2; -.
DR   STRING; 5762.D2V4D2; -.
DR   EnsemblProtists; EFC48495; EFC48495; NAEGRDRAFT_78524.
DR   GeneID; 8862122; -.
DR   VEuPathDB; AmoebaDB:NAEGRDRAFT_78524; -.
DR   eggNOG; KOG3629; Eukaryota.
DR   InParanoid; D2V4D2; -.
DR   OrthoDB; 7992at2759; -.
DR   Proteomes; UP000006671; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR   PANTHER; PTHR23113:SF312; RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE, ISOFORM E; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SUPFAM; SSF48366; Ras GEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
PE   4: Predicted;
KW   Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW   ECO:0000256|PROSITE-ProRule:PRU00168}; Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006671};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        57..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          481..734
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000259|PROSITE:PS50009"
FT   REGION          171..207
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          739..794
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        178..207
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        741..788
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   915 AA;  103463 MW;  E4F5497AF876DA18 CRC64;
     MTICFGDDSF ISSNSLSSME ETNSPNSNNI KASSAAIVHT TTSYNNNSTT GNNLEQILII
     SLSSGLGGFF LVLLLIGAVF VFIKIYCKRK SNNKKKIHSH RDGIAIRVGD DEDGISSVMD
     GQSNDGSIAG SFSRNEDDSG RLLKNRVSSQ QVMSIDNDDQ YRIDTTRFME GGGHDNYQFP
     TPQHQQQQEN NGKYHRKFNS SPSSSPLINR EDIGGLVNYQ TVPSTSLLLS MIQQDSKKPT
     IMGCDDYLGE QNVANSELLI HIRNHHIPKC VSSIRNTNVE SANCVNWSCH ESGDISKELI
     LKDSVSVENS NFELEHLKSI TLTGLFKLSE TNDNIRRVLF DLHLYIFPNT HLFVNSLLSL
     EDCELRKSFI EYFLRYYSRD LDSFDEYCTL SELVGVKINQ ENFKNSCIHY ANEFRSWSYP
     NTFTSWSEFL KSFEGSFEAS STPNHPSNKK IELDKSIIKP IASYLDEFSL IKSPLVLSIA
     NPEIIAQHLT AMDALLLRNV NIHKELTLER YANVNCQTTP LYKTDLQYST SQCPSLSLYI
     DYFNKLGIFV KFCIFYKNLS LQERASCIEK FLDICQHLEK LGNYNSLMAI YGSLTSQCVS
     RLKNVWSKVE QKYQDYLTTL DSLFMIRKKF ENLRVAQFTR YTPIIPYFGL FMTEMTQIES
     IHSTTIQNTE HRDKSFFNMV KLGQIYRIHQ QIETCQSYAN QYLSLPIDTV LFNRLQEEII
     GFDLLKLELL FEKKEKSPSR SISMDSGCTS TSSGTDTSLT PRLSVHNDEA TLVIPSESVT
     SPPTTPRGKK KNISELADKE FATLSVLICP KRSNSQTGGT PNRLTADSFI GGLWEKLSPK
     RLSFSNFTLP VPQITDGGPT SPRRSSVTTV SPRLLSVEDA HHHLKKFSDS DSKLLQSKQI
     SNLNVITSPR LLETK
//