ID D3EMV7_ATETH Unreviewed; 1151 AA.
AC D3EMV7;
DT 23-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 23-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN OrderedLocusNames=UCYN_00510 {ECO:0000313|EMBL:ADB94807.1};
OS Atelocyanobacterium thalassa (isolate ALOHA).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Aphanothecaceae; Atelocyanobacterium;
OC Candidatus Atelocyanobacterium thalassa.
OX NCBI_TaxID=1453429 {ECO:0000313|Proteomes:UP000001405};
RN [1] {ECO:0000313|EMBL:ADB94807.1, ECO:0000313|Proteomes:UP000001405}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ALOHA {ECO:0000313|Proteomes:UP000001405};
RX PubMed=20173737; DOI=10.1038/nature08786;
RA Tripp H.J., Bench S.R., Turk K.A., Foster R.A., Desany B.A., Niazi F.,
RA Affourtit J.P., Zehr J.P.;
RT "Metabolic streamlining in an open-ocean nitrogen-fixing cyanobacterium.";
RL Nature 464:90-94(2010).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; CP001842; ADB94807.1; -; Genomic_DNA.
DR RefSeq; WP_012953472.1; NC_013771.1.
DR AlphaFoldDB; D3EMV7; -.
DR STRING; 1453429.UCYN_00510; -.
DR KEGG; cyu:UCYN_00510; -.
DR PATRIC; fig|713887.8.peg.42; -.
DR HOGENOM; CLU_005122_1_0_3; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000001405; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000001405}.
FT DOMAIN 621..783
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 803..958
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1151 AA; 131192 MW; 0650FC09C083BA25 CRC64;
MVFSSLIHTL QSSSLIKNLS QQLKVKNELR LQGINRLSKG LISSVISQTN YQNLLVICAT
SEEAGRWTKQ LEIMGWKDIN FYFVPDTSPY EELNIENEII WTQMQVLSNL FRAKQSKDTN
FAIVTTERSL QPHLPSPSIF YDYCLNFKVG VTVYSKDFAQ TLSELGYKKV NLVEKEGEWS
RRGDILDIFP VSSEIPIRLE WLGDELEKIR EFDISTQRSL NEIEEALLTP INFNIIAKDI
IQKNPFSSEQ YVVEEKPKTL KNSSPKDQLP LSKSSSLFDY LPSDTLCAFD EIDQCKSHNH
KWLEYVEKSW HETQNKEPKV HRSFEESFSS IEEMPKLYLS ELSDVNETDS IDILSRSIPV
NPHQFSKLAE ILRGKREIFN EINISKYSIW LISAQPSRTV TLLQEHDYSA QFIPNIRDYP
AIKKSHAQGM AVALKHSGLV GLEGFILPIF RIAVITDKEF FGQSTLGSSE FFKKRRRSNA
KKVNLQKLNS GDHVVHKSHG IGKFLRLENL SNREYLVVQY SDGVLRVPAD SLDNLLRYSC
TDSTPPKLHK MTGKDWGKLK QKIRKNIKKL AFNLLHLYAE RAQKKGYSYP ADSLWQQELE
DSFPYQATPD QVQAIREVKI DLESNRPMDR LICGDVGFGK TEVAIRAIFK VVTSGHKQVV
FLAPTTILTQ QHYNTLRTRF APYPISIGLL NRFRTTSEKK DIIERLTTGE LDIVVGTQQL
LGKNIKFKDL GLLVIDEEQR FGVNHKEKIR TIKANVDVLT LTATPIPRTL HMSLSGIREM
SLISTPPPSR RSIKTHLSSY DPNLVKAAVR AELDRGGQVF YVVPRIEHID ELVLQLKRMI
PDAKILVAHG QMDVNNLELT MLSFNNGDAD ILVCTTIIES GLDIPKVNTI IIEDAQKFGL
SQLYQLRGRV GRSGIQAHAW LFYPSRSELT DNALKRLNAL QEFSELGSGY HLATRDLEIR
GAGNLLGAEQ SGQMEAIGFE LYMDMLQEAI KEVQGEKIPE VEDTQIDLKL TAFIPNDYIA
DIEQKIIAYR TVAVASSKKE LDLIVAEWSE RYGSIPDPVK QLLKTIELKQ LSKSLGFSHI
KIEGKNSVVL KTPMEEPAWI ILQNKLPEHL QTRFVYNSKK IIIRGLGSLS PQKQLESLLV
WFKIMQENEK V
//
