ID   D3HX54_9BACT            Unreviewed;       561 AA.
AC   D3HX54;
DT   23-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   23-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 55.
DE   SubName: Full=Glycosyl hydrolase, family 43 {ECO:0000313|EMBL:EFC76214.1};
GN   ORFNames=HMPREF0649_00836 {ECO:0000313|EMBL:EFC76214.1};
OS   Segatella buccae D17.
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC   Segatella.
OX   NCBI_TaxID=575611 {ECO:0000313|EMBL:EFC76214.1, ECO:0000313|Proteomes:UP000005080};
RN   [1] {ECO:0000313|EMBL:EFC76214.1, ECO:0000313|Proteomes:UP000005080}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=D17 {ECO:0000313|EMBL:EFC76214.1,
RC   ECO:0000313|Proteomes:UP000005080};
RG   The Broad Institute Genome Sequencing Platform;
RA   Ward D., Feldgarden M., Earl A., Young S.K., Zeng Q., Koehrsen M.,
RA   Alvarado L., Berlin A., Bochicchio J., Borenstein D., Chapman S.B.,
RA   Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA   Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D., Larson L.,
RA   Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T.,
RA   Shenoy N., Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J.,
RA   Yandava C., Sibley C.D., White A., Allen-Vercoe E., Strauss J., Crowley S.,
RA   Surette M.G., Haas B., Nusbaum C., Birren B.;
RT   "The Genome Sequence of Prevotella buccae Oral Taxon 560 strain D17.";
RL   Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR   EMBL; GG739929; EFC76214.1; -; Genomic_DNA.
DR   RefSeq; WP_004341400.1; NZ_GG739929.1.
DR   AlphaFoldDB; D3HX54; -.
DR   HOGENOM; CLU_016508_2_2_10; -.
DR   OrthoDB; 9801455at2; -.
DR   Proteomes; UP000005080; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd18617; GH43_XynB-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..561
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003046393"
FT   DOMAIN          382..560
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        58
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        231
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            166
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   561 AA;  62511 MW;  384A837844B3EA53 CRC64;
     MNKKTLLGAA LLLVSLAPRA QQATFSYFSY QGDEPRFRKQ IDTGREFFNP ILAGFYPDPS
     ICRVGDTYYM VNSSFSFFPG VPLSTSKDLV HWTPAGHVLD RPSQVPLARQ KVSGGIFAPA
     ISYNPKNKTF YMITTNVGAG NFYVKSKDPA KGWSEPIYLR KIDGIDPSFF FDKDGAGYIV
     HNAPVDGGAD YEGQRAIRIF RFDTEGDSIV GDYKQIVRGG THVEKRPIWI EGPHLYRIGK
     YYYLMCAEGG TGDWHSEVIF RSKSPMGPWE ENPDNPILTQ RTGLDPNRPD IVTSTGHADL
     VQSKEGDWWA VFLGCRPYEA DYYNTGRDTY LLPVAWKNGW PVILDKEKAV PTVLAMKPWQ
     QKMQKAVPVG AVATTGNFAY TDRFVGDRLD GRWMFLRNPS QFYSLGTDGL TINALPADIS
     QREPLSAVFC RQQHTVFSAE TRVAFHPASD KQLAGLALLQ NEEYNFVMGK TRLNGRLAVV
     LTRSERVNAV VASAFLDEAD ADKPLRLKID GHGRYYDFYY AVADGPWQLL ARGVDAVNLS
     THRSGGFIGA CIGLYATAKG S
//