ID D3R020_MAGIU Unreviewed; 517 AA.
AC D3R020;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=Alpha-amylase {ECO:0000256|RuleBase:RU361134};
DE EC=3.2.1.1 {ECO:0000256|RuleBase:RU361134};
GN OrderedLocusNames=HMPREF0868_0185 {ECO:0000313|EMBL:ADC91848.1};
OS Mageeibacillus indolicus (strain UPII9-5) (Clostridiales genomosp. BVAB3
OS (strain UPII9-5)).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Mageeibacillus.
OX NCBI_TaxID=699246 {ECO:0000313|EMBL:ADC91848.1, ECO:0000313|Proteomes:UP000008234};
RN [1] {ECO:0000313|Proteomes:UP000008234}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UPII9-5 {ECO:0000313|Proteomes:UP000008234};
RA Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA Strausberg R.L., Nelson K.E.;
RT "Sequence of Clostridiales genomosp. BVAB3 str. UPII9-5.";
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC polysaccharides containing three or more (1->4)-alpha-linked D-
CC glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|RuleBase:RU361134};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
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DR EMBL; CP001850; ADC91848.1; -; Genomic_DNA.
DR AlphaFoldDB; D3R020; -.
DR STRING; 699246.HMPREF0868_0185; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR KEGG; clo:HMPREF0868_0185; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_006462_2_4_9; -.
DR Proteomes; UP000008234; Chromosome.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd11316; AmyAc_bac2_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR006046; Alpha_amylase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR PANTHER; PTHR10357:SF179; NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PRINTS; PR00110; ALPHAAMYLASE.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361134};
KW Glycosidase {ECO:0000256|RuleBase:RU361134};
KW Hydrolase {ECO:0000256|RuleBase:RU361134};
KW Reference proteome {ECO:0000313|Proteomes:UP000008234};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..517
FT /note="Alpha-amylase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039398103"
FT DOMAIN 47..427
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 517 AA; 57685 MW; B4DADEFD11AA813A CRC64;
MKMKKIVSFL LCLCLVLAGC VGCRWSKGGD LPHDSSNIKT RSGAVYEIYV ASFNDSDGDQ
VGDLNGIKEK LEYLHTLGVE YIWLMPIHPS HSYHKYDIDD YMAIDPAYGT MDDFRGLIKA
AEAYGIKIIM DMVINHTSSH HPWFQQALAE AMKGKMDGKA EFYNFSNKPI RKSTKVKDGL
YYESAFVSEM PDLNLGNPQV KAELEKIAAF YLKMGVAGFR LDAALHYFGT DNKANIAFLK
WYGDMCRKYR PDVYLTAEVW SDSATVESYY ASGVDSFFNF ALSEGKGLFA RAINAGRGAD
LAKYLADYDA SIHKLNPQAL NAVYLSNHDQ GRSAGFLPEL AKNKLAEAMY IWLPGRPYLY
YGEEVRLKGS GKDENKRTYM PWNASTKVKN PVGTDYDQAK QVTETVEAAV ANPDSLLNYI
EELLAVRNSY PWLERGVPTA VPADERLLVL EVNGRNGEGR AVIVHNLSAD MVEFSPSGEH
LRPAKLMNKA DKFNHGCLQL APYSSAFLVE VSGEGKK
//
