ID D3TSB7_GLOMM Unreviewed; 567 AA.
AC D3TSB7;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=E1 ubiquitin-activating enzyme {ECO:0000256|ARBA:ARBA00012990};
DE EC=6.2.1.45 {ECO:0000256|ARBA:ARBA00012990};
OS Glossina morsitans morsitans (Savannah tsetse fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Hippoboscoidea;
OC Glossinidae; Glossina.
OX NCBI_TaxID=37546 {ECO:0000313|EMBL:ADD20595.1};
RN [1] {ECO:0000313|EMBL:ADD20595.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Salivary gland {ECO:0000313|EMBL:ADD20595.1};
RX PubMed=20353571; DOI=10.1186/1471-2164-11-213;
RA Alves-Silva J., Ribeiro J.M., Van Den Abbeele J., Attardo G., Hao Z.,
RA Haines L.R., Soares M.B., Berriman M., Aksoy S., Lehane M.J.;
RT "An insight into the sialome of Glossina morsitans morsitans.";
RL BMC Genomics 11:213-213(2010).
RN [2] {ECO:0000313|EMBL:ADD20595.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Salivary gland {ECO:0000313|EMBL:ADD20595.1};
RG International Glossina Genome Initiative;
RA da Silva J., Ribeiro J.M.C., Abbeele J.V., Attardo G., Hao Z., Haines L.R.,
RA Soares M.B., Berriman M., Aksoy S., Lehane M.J.;
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
CC = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-
CC L-cysteine.; EC=6.2.1.45; Evidence={ECO:0000256|ARBA:ARBA00000488};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family.
CC {ECO:0000256|ARBA:ARBA00005673}.
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DR EMBL; EZ424319; ADD20595.1; -; mRNA.
DR AlphaFoldDB; D3TSB7; -.
DR UniPathway; UPA00143; -.
DR GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd01490; Ube1_repeat2; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 1.10.10.2660; Ubiquitin-activating enzyme E1, SCCH domain; 1.
DR Gene3D; 3.10.290.60; Ubiquitin-activating enzyme E1, UFD domain; 1.
DR InterPro; IPR045886; ThiF/MoeB/HesA.
DR InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR InterPro; IPR018965; Ub-activating_enz_E1_C.
DR InterPro; IPR038252; UBA_E1_C_sf.
DR InterPro; IPR019572; UBA_E1_SCCH.
DR InterPro; IPR042063; Ubi_acti_E1_SCCH.
DR InterPro; IPR035985; Ubiquitin-activating_enz.
DR InterPro; IPR033127; UBQ-activ_enz_E1_Cys_AS.
DR InterPro; IPR000011; UBQ/SUMO-activ_enz_E1-like.
DR PANTHER; PTHR10953:SF4; UBA_E1_C DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR10953; UBIQUITIN-ACTIVATING ENZYME E1; 1.
DR Pfam; PF09358; E1_UFD; 1.
DR Pfam; PF00899; ThiF; 1.
DR Pfam; PF10585; UBA_E1_SCCH; 1.
DR PRINTS; PR01849; UBIQUITINACT.
DR SMART; SM00985; UBA_e1_C; 1.
DR SUPFAM; SSF69572; Activating enzymes of the ubiquitin-like proteins; 1.
DR PROSITE; PS00865; UBIQUITIN_ACTIVAT_2; 1.
PE 2: Evidence at transcript level;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 437..562
FT /note="Ubiquitin-activating enzyme E1 C-terminal"
FT /evidence="ECO:0000259|SMART:SM00985"
FT ACT_SITE 143
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10132"
SQ SEQUENCE 567 AA; 64425 MW; C2E4E7B748BD1D96 CRC64;
MIGAGTRNGQ LFITDMDLIE KSNLNRQFLF RPKDVQKPKA QTAAAAIRMM NPEAKVTAYE
LRVGSETERV FSEEFFGKLD GVTNALDNVD ARIYMDRKCV FNRIPLVESG TLGTMGNVQV
IVPFLTESYS SSQDPPEKSI PICTLKNFPN AIEHTLQWAR DCFEGVFSQT AENAALYITD
PNFIERTLKL TGIQPLEILE SVKKALIDNK PKDFADCIEW ARLYWEEQYA NQIKQLLYNF
PPDQVTSSGQ PFWSGPKRCP EPLLFDVTNS LHLDFVYAAS NLMAQVYGIP QMRDREYIAC
KLQGVQVPEF QPRSGVKIET NEAAAAAEAN NHDGSEVDQD RVNKILNELQ ALGKLNFSIT
PLQFEKDDDT NLHMDFIVAA SNLRAANYKI PPADRHKSKL IAGKIIPAIA TTTSLVSGWV
VLEVIKLIMG HKDLSKFKNG FANLALPFIT FSEPKRAESN TYYDKNWTLW DRFEVPGEMT
LQEFLDYFEK KEKLKITMLS QGISMLYSFF MPKAKCAERL PLPMSEVVRR VSKKRIEPHE
RSLVFEICCN DEEGEDVEVP YVRYTLP
//
