ID D4E061_SEROD Unreviewed; 81 AA.
AC D4E061;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Sulfur carrier protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur mediator TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur transfer protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000256|HAMAP-Rule:MF_00413};
GN Name=tusA {ECO:0000256|HAMAP-Rule:MF_00413,
GN ECO:0000313|EMBL:EFE96967.1};
GN ORFNames=HMPREF0758_1561 {ECO:0000313|EMBL:EFE96967.1};
OS Serratia odorifera DSM 4582.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=667129 {ECO:0000313|EMBL:EFE96967.1, ECO:0000313|Proteomes:UP000005723};
RN [1] {ECO:0000313|EMBL:EFE96967.1, ECO:0000313|Proteomes:UP000005723}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 4582 {ECO:0000313|EMBL:EFE96967.1,
RC ECO:0000313|Proteomes:UP000005723};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC cell. Part of a sulfur-relay system required for 2-thiolation during
CC synthesis of 2-thiouridine of the modified wobble base 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC IscS and stimulates its cysteine desulfurase activity. Accepts an
CC activated sulfur from IscS, which is then transferred to TusD, and thus
CC determines the direction of sulfur flow from IscS to 2-thiouridine
CC formation. Also appears to be involved in sulfur transfer for the
CC biosynthesis of molybdopterin. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- PATHWAY: tRNA modification. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SUBUNIT: Interacts with IscS. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC {ECO:0000256|ARBA:ARBA00008984, ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFE96967.1}.
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DR EMBL; ADBY01000025; EFE96967.1; -; Genomic_DNA.
DR RefSeq; WP_004957449.1; NZ_GG753567.1.
DR AlphaFoldDB; D4E061; -.
DR STRING; 667129.HMPREF0758_1561; -.
DR HOGENOM; CLU_165255_5_0_6; -.
DR OrthoDB; 9797352at2; -.
DR Proteomes; UP000005723; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR CDD; cd03423; SirA; 1.
DR Gene3D; 3.30.110.40; TusA-like domain; 1.
DR HAMAP; MF_00413; Thiourid_synth_A; 1.
DR InterPro; IPR022931; Sulphur_carrier_TusA.
DR InterPro; IPR001455; TusA-like.
DR InterPro; IPR036868; TusA-like_sf.
DR PANTHER; PTHR33279:SF6; SULFUR CARRIER PROTEIN TUSA; 1.
DR PANTHER; PTHR33279; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR Pfam; PF01206; TusA; 1.
DR SUPFAM; SSF64307; SirA-like; 1.
DR PROSITE; PS01148; UPF0033; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413};
KW Reference proteome {ECO:0000313|Proteomes:UP000005723};
KW Transferase {ECO:0000313|EMBL:EFE96967.1};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_00413}.
FT DOMAIN 12..36
FT /note="UPF0033"
FT /evidence="ECO:0000259|PROSITE:PS01148"
FT ACT_SITE 19
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00413"
SQ SEQUENCE 81 AA; 9283 MW; 42AB33A06518B788 CRC64;
MTDIFSEADQ TLDALGLRCP EPVMMVRKTV RHMDNGETLL IIADDPATTR DIPGFCRFME
HTLVAQETER APYRYLLRKG L
//
