ID   D4KZC7_9FIRM            Unreviewed;       581 AA.
AC   D4KZC7;
DT   18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT   18-MAY-2010, sequence version 1.
DT   24-JAN-2024, entry version 56.
DE   SubName: Full=Beta-galactosidase {ECO:0000313|EMBL:CBL12717.1};
DE            EC=3.2.1.23 {ECO:0000313|EMBL:CBL12717.1};
GN   ORFNames=RO1_22090 {ECO:0000313|EMBL:CBL12717.1};
OS   Roseburia intestinalis XB6B4.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX   NCBI_TaxID=718255 {ECO:0000313|EMBL:CBL12717.1, ECO:0000313|Proteomes:UP000008953};
RN   [1] {ECO:0000313|EMBL:CBL12717.1, ECO:0000313|Proteomes:UP000008953}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=XB6B4 {ECO:0000313|EMBL:CBL12717.1,
RC   ECO:0000313|Proteomes:UP000008953};
RG   metaHIT consortium -- http://www.metahit.eu/;
RA   Pajon A., Turner K., Parkhill J., Bernalier A.;
RT   "The genome sequence of Roseburia intestinalis XB6B4.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBL12717.1, ECO:0000313|Proteomes:UP000008953}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=XB6B4 {ECO:0000313|EMBL:CBL12717.1,
RC   ECO:0000313|Proteomes:UP000008953};
RA   Pajon A.;
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809}.
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DR   EMBL; FP929050; CBL12717.1; -; Genomic_DNA.
DR   RefSeq; WP_015521234.1; NC_021012.1.
DR   AlphaFoldDB; D4KZC7; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   GeneID; 61434360; -.
DR   KEGG; rix:RO1_22090; -.
DR   PATRIC; fig|718255.3.peg.3371; -.
DR   HOGENOM; CLU_007853_7_2_9; -.
DR   Proteomes; UP000008953; Chromosome.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR048912; BetaGal1-like_ABD1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21317; BetaGal_ABD_1; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:CBL12717.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CBL12717.1}.
FT   DOMAIN          10..323
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          370..480
FT                   /note="Beta-galactosidase 1-like first all-beta"
FT                   /evidence="ECO:0000259|Pfam:PF21317"
FT   DOMAIN          499..557
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
FT   ACT_SITE        157
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT   ACT_SITE        233
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ   SEQUENCE   581 AA;  67218 MW;  20D5A06B20346BFF CRC64;
     MGNFEITDNF YLDGKPFQII SGAIHYFRIV PEYWQDRLEK LKAMGCNTVE TYIPWNMHEP
     KKGEFHFEGM LDIERFVKTA QELGLYVILR PSPYICAEWE FGGLPAWLLA EDGMKLRVSY
     PPFLKHVQDY YDVLLKKIVP YQINYGGPVI LMQVENEYGY YANDREYLLA MRDKMQKGGV
     VVPLVTSDGP FEENLNGGHL EGALPTGNFG SKTEERFEVL KKYTDGGPLM CTEFWVGWFD
     HWGNGGHMTG NLEESVKDLD KMLELGHVNI YMFEGGTNFG FMNGSNYYDE LTPDVTSYDY
     DALLTEDGQI TEKYRRYRDV IAKYREIPEV TFTTEIKRKA YGSLPLKEKV SLFSVLDDIS
     APVESSFLQS MEKLGQNYGY ILYHSTLDTE EKLEKLRLWE ANDRANIFVD QKPVTTLYDL
     ELLKEKELDV TFERGADFDI LMENMGRVNF GPRMEHQRKG IGQCVQVNGH MHNHWKQYTL
     PLDNIEKVDF SKEYKEGLPG FYRFTVDIDE TADTFLDFEG WGKGCVFVNG FNIGRFWEIG
     PQKRLYIPAP LLKKGENEII VFETEGKTRD TIVLKDEPDI G
//